Chloroquine mediates specific proteome oxidative damage across the erythrocytic cycle of resistant Plasmodium falciparum

Differential carbonylation of cytoskeletal proteins in blood group O erythrocytes: Potential role in protection against severe malaria

“…The altered redox status in the parasite and host red cell (Akide-Ndunge et al, 2009;Mendez et al, 2011;Radfar et al, 2008) during invasion seems to contribute to the severe manifestations of disease and, contrastingly, exacerbated oxidative stress induces a potentially beneficial immunoregulation mechanism in the host (Becker et al, 2004;Cappadoro et al, 1998;Ferreira et al, 2008Ferreira et al, , 2011.…”

Modification of oxidative status in Plasmodium berghei-infected erythrocytes by E-2-chloro-8-methyl-3-[(4'-methoxy-1'-indanoyl)-2'-methyliden]-quinoline compared to chloroquine

“…Indeed, part of the biological activity of CQ results from the augmentation of oxidative stress within the parasites (Toler et al 2006). This drug leads to increases in the number of oxidised proteins as the intraerythrocytic cycle progresses to mature stages (Radfar et al 2008). Additionally, CQ treatment mediated oxidative stress in the host; this effect was exacerbated in Plasmodium falciparum infected patients administered with the drug (Farombi et al 2003).…”

Caracterización parcial del proteoma del trofozoito de Plasmodium falciparum bajo tratamiento con quinina, mefloquina y el antiplasmodial natural diosgenona

“…Parasites were cultured in vitro under standard conditions (13), synchronized as described previously (14), with modifications (8) to render 500 µg of protein per 100 ml of culture. Parasites were maintained in human red blood cells type A+ in RPMI 1640 (Sigma, USA), supplemented with 10% human serum at 1% v/v hematocrit, in an atmosphere of 90% N 2 , 5% O 2 and 5% CO 2 , at 37°C.…”

“…Proteomic approaches have allowed the understanding of the molecular events occurring in the parasite in response to different antimalarial drugs and environments. However, some are based on different proteomic techniques with contrasting results (4)(5)(6)(7)(8)(9)(10)(11).…”