Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers

Kwak, Seon‐Yeong; Lew, Tedrick Thomas Salim; Sweeney, Connor J.; Koman, Volodymyr B.; Wong, Min Hao; Bohmert-Tatarev, Karen; Snell, Kristi D.; Seo, Jun Sung; Chua, Nam‐Hai; Strano, Michael S.

doi:10.1038/s41565-019-0375-4

Cited by 387 publications

(319 citation statements)

References 59 publications

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“…As discussed in the introduction, transfection agents that are used in animal systems may have limited efficacy in plants due to the cell wall size limitations. Recently, there have been several reports describing the use of nanostructures for delivery of nucleic acids into plant cells , Kwak et al, 2019. Another class of small nanoparticles called carbon dots have been used in animal systems for bio-imaging, delivery of various drugs, and the delivery of nucleic acids.…”

Section: Discussionmentioning

confidence: 99%

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Carbon dots for efficient siRNA delivery and gene silencing in plants

Schwartz

Hendrix

Hoffer

et al. 2019

Preprint

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The Initiation of RNA interference (RNAi) by topically applied double stranded RNA (dsRNA) has potential applications for plant functional genomics, crop improvement and crop protection. The primary obstacle for the development of this technology is efficient delivery of RNAi effectors. The plant cell wall is a particularly challenging barrier to the delivery of macromolecules. Many of the transfection agents that are commonly used with animal cells produce nanocomplexes that are significantly larger than the size exclusion limit of the plant cell wall. Utilizing a class of very small nanoparticles called carbon dots, a method of delivering siRNA into the model plant Nicotiana benthamiana and tomato is described. Low-pressure spray application of these formulations with a spreading surfactant resulted in strong silencing of GFP transgenes in both species. The delivery efficacy of carbon dot formulations was also demonstrated by silencing endogenous genes that encode two sub-units of magnesium chelatase, an enzyme necessary for chlorophyll synthesis. The strong visible phenotypes observed with the carbon dot facilitated delivery were validated by measuring significant reductions in the target gene transcript and/or protein levels. Methods for the delivery of RNAi effectors into plants, such as the carbon dot formulations described here, could become valuable tools for gene silencing in plants with practical applications in plant functional genomics and agriculture.

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Section: Discussionmentioning

confidence: 99%

“…This is 10 to 20-fold larger than the size exclusion of the plant cell wall. Recently, there have been several reports of using smaller nanostructures such as single-walled carbon nanotubes , Kwak et al, 2019 and DNA nanoparticles for delivery of nucleic acids into plant cells.…”

Section: Introductionmentioning

confidence: 99%

Carbon dots for efficient siRNA delivery and gene silencing in plants

Schwartz

Hendrix

Hoffer

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…An in vitro study showed a three‐fold increase in photosynthetic activity and a greatly accelerated rate of electron transport in an isolated chloroplast embedded with single walled carbon nanotubes abbreviated as SWCNTs . SWCNTs also allowed the transmission of signal molecules such as nitric oxide by plants . Noji et al reported that photosynthetic oxygen‐evolving reaction was achieved with a stable activity when a nanomesoporous silica compound was bound to photosystem II (PSII) .…”

Section: Np Effects On Plant Growth and Mechanism Of Actionmentioning

confidence: 99%

Nanoparticle–Plant Interactions: Two‐Way Traffic

Khan

Adam

Rizvi

et al. 2019

Small

147

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In this Review, an effort is made to discuss the most recent progress and future trend in the two‐way traffic of the interactions between plants and nanoparticles (NPs). One way is the use of plants to synthesize NPs in an environmentally benign manner with a focus on the mechanism and optimization of the synthesis. Another way is the effects of synthetic NPs on plant fate with a focus on the transport mechanisms of NPs within plants as well as NP‐mediated seed germination and plant development. When NPs are in soil, they can be adsorbed at the root surface, followed by their uptake and inter/intracellular movement in the plant tissues. NPs may also be taken up by foliage under aerial deposition, largely through stomata, trichomes, and cuticles, but the exact mode of NP entry into plants is not well documented. The NP–plant interactions may lead to inhibitory or stimulatory effects on seed germination and plant development, depending on NP compositions, concentrations, and plant species. In numerous cases, radiation‐absorbing efficiency, CO2 assimilation capacity, and delay of chloroplast aging have been reported in the plant response to NP treatments, although the mechanisms involved in these processes remain to be studied.

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“…This review will focus on the emerging applications of plant nanobionics and bioelectronic interface to provide functionalities typically obtained from conventional electronic devices. We specifically highlight the use of species‐independent technologies that can be applied to any wild‐type plants without the need of genetic engineering method which is only applicable to a narrow range of plant species . This reflects the increasing use of wild‐type plants for field‐based solutions in areas where genetic engineering approaches are often ineffective due to regulatory oversight, public scrutiny, and the time‐consuming processes associated with plant breeding .…”

Section: Introductionmentioning

confidence: 99%

The Emergence of Plant Nanobionics and Living Plants as Technology

Lew

Koman

Gordiichuk

et al. 2019

Adv Materials Technologies

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Plants are naturally abundant and display high sensitivity to ecological factors to thrive in diverse environmental conditions. As sessile organisms, they have evolved complex, internal, and interplant signaling pathways with distinct structures to promptly adjust to the constantly changing environment. In the past five years, the unique ways in which they exchange information with and function in the environment have inspired an emerging field of plant nanobionics, which describes the interface between living plants and nanotechnology to impart the former with novel and useful functions. The structural merits of plant organs and organelles have also inspired the creation of plant‐derived structures through biointerfacing with nanoparticles containing electronic and optical properties. Here, the emerging applications and vision of plant nanobionics are highlighted together with related plant‐inspired materials in potentially replacing the myriad devices in the everyday lives stamped out of plastic, containing circuit boards and consuming power from the electrical grid. Applications in environmental sensing, communication devices, and energy harvesting and conversion are comprehensively discussed.

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Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers

Cited by 387 publications

References 59 publications

Carbon dots for efficient siRNA delivery and gene silencing in plants

Carbon dots for efficient siRNA delivery and gene silencing in plants

Nanoparticle–Plant Interactions: Two‐Way Traffic

The Emergence of Plant Nanobionics and Living Plants as Technology

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