Chloroplast pigments, proteins, lipid peroxidation and activities of antioxidative enzymes during maturation and senescence of leaves and reproductive organs of Cajanus cajan L.

Jakhar, Somveer; Mukherjee, D.

doi:10.1007/s12298-013-0219-x

Cited by 28 publications

(19 citation statements)

References 40 publications

(37 reference statements)

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“…In Zea mays 11 , Sorghum bicolor 12 , and Arabidopsis thaliana 13 , chlorophyll contents were closely related to leaf senescence. Besides, during leaf senescence, some genes involved in photosynthesis would be down regulated expressed, resulting in the decrease of the photosynthetic ability of leaves 14,15 . Chlorophyll binding protein genes were in a down regulated trend during the senescence process of Oryza Sativa 16 and Gossypium 17 .…”

Section: Discussionmentioning

confidence: 99%

Study on the Anti-Aging Physiological Characteristics and Molecular Mechanism of Camellia Oleifera

Chen¹,

Chen

Zhang³

et al. 2021

Preprint

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To study the physiological and molecular regulating mechanism of ancient Camellia oleifera which kept a exuberant vitality for more than one hundred years, leaves of 30a year old and > 100 year old Camellia oleifera were selected as targets. On the basis of the study of the generation and the clearance of reactive oxygen species, sequencing analysis of the transcriptome and expression profiling by high throughput sequencing analysis technique was conducted to study differentially expressed functional genes related to the tree age. It showed that the chlorophyll content and enzyme activities increased in ancient Camellia oleifera leaves. Expression of chlorophyll a/b binding protein gene, auxin related gene, the signal transduction factor and the transcription factor gene in ancient trees were all higher than mature tree. The down regulated gene expression of inductive genes related to protein degradation in ancient tree. Under the comprehensive function of those factors, ancient Camellia oleifera leaves still kept an exuberant vitality which was very useful for studies of stress resistance molecular biology and genetic improvement of Camellia oleifera.

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Section: Discussionmentioning

confidence: 99%

Study on the Anti-Aging Physiological Characteristics and Molecular Mechanism of Camellia Oleifera

Chen¹,

Chen

Zhang³

et al. 2021

Preprint

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“…Previous studies have investigated the relationships between cell proliferation and the accumulation of oxidative damage in photosynthetic organisms and have revealed that an increase in intracellular lipid peroxide (LPO) decreases survival rates and potentials for growth during certain phases of growth and under various stressful conditions including postflooding, exposure to herbicides, and exposure to ultraviolet radiation [ 66 , 67 , 68 , 69 ]. Hurng and Kao [ 67 ] reported an increase in LPO activity and decrease in SOD and CAT activities in tobacco leaves after flooding.…”

Section: Effect Of 2-cysprx Protein Levels On Survival and Productmentioning

confidence: 99%

“…The large amounts of ROS, such as H 2 O 2 , •OH, and 1 O 2 , produced under excessive irradiance should increase the risk of LPO production. Accumulation of LPO increases with increased oxidative stress [ 66 , 67 , 68 , 69 ]. Because the H 2 O 2 concentration was higher in the high-irradiance treatment than in the low-irradiance treatment, the increased LPO in C. mairna might have been caused by excessive irradiance.…”

Section: Effect Of 2-cysprx Protein Levels On Survival and Productmentioning

confidence: 99%

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

et al. 2021

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Peroxiredoxin (Prx) is a relatively recently discovered antioxidant enzyme family that scavenges peroxides and is known to be present in organisms from biological taxa ranging from bacteria to multicellular eukaryotes, including photosynthetic organisms. Although there have been many studies of the Prx family in higher plants, green algae, and cyanobacteria, few studies have concerned raphidophytes and dinoflagellates, which are among the eukaryotic algae that cause harmful algal blooms (HABs). In our proteomic study using 2-D electrophoresis, we found a highly expressed 2-Cys peroxiredoxin (2-CysPrx) in the raphidophyte Chattonella marina var. antiqua, a species that induces mass mortality of aquacultured fish. The abundance of the C. marina 2-CysPrx enzyme was highest in the exponential growth phase, during which photosynthetic activity was high, and it then decreased by about a factor of two during the late stationary growth phase. This pattern suggested that 2-CysPrx is a key enzyme involved in the maintenance of high photosynthesis activity. In addition, the fact that the depression of photosynthesis by excessively high irradiance was more severe in the 2-CysPrx low-expression strain (wild type) than in the normal-expression strain (wild type) of C. marina suggested that 2-CysPrx played a critical role in protecting the cell from oxidative stress caused by exposure to excessively high irradiance. In the field of HAB research, estimates of growth potential have been desired to predict the population dynamics of HABs for mitigating damage to fisheries. Therefore, omics approaches have recently begun to be applied to elucidate the physiology of the growth of HAB species. In this review, we describe the progress we have made using a molecular physiological approach to identify the roles of 2-CysPrx and other antioxidant enzymes in mitigating environmental stress associated with strong light and high temperatures and resultant oxidative stress. We also describe results of a survey of expressed Prx genes and their growth-phase-dependent behavior in C. marina using RNA-seq analysis. Finally, we speculate about the function of these genes and the ecological significance of 2-CysPrx, such as its involvement in circadian rhythms and the toxicity of C. marina to fish.

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“…LOX was one of the few proteins in the mutant whose expression decreased. Lipid peroxidation is initiated by LOX and is associated with the formation of hydroperoxy fatty acids, reactive carbonyl species (RCS), and 1 O2 (Jakhar et al 2014). A highly upregulated protein is alkenal/one oxidoreductase (AOR), which catalyzes RCS using NADPH as the reductant in the PSII (Pospíšil and Yamamoto 2017), suggesting that the differentially expressed LOX and AOR in the mutant help decrease lipid peroxidation products in PSII.…”

Section: Catalase and Lipoxygenase And Cinnamate-4-hydro-mentioning

confidence: 99%

Proteomic analysis of chloroplasts from chlorophyll-deficient melon mutant

Zhao¹,

Cui²,

Li³

et al. 2019

Photosynt.

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We aimed to understand the molecular-level changes occurring in the photosynthetic metabolic network in mutant chloroplasts. We performed comparative liquid chromatography-mass spectrometry protein profiling of wild-type (WT) and chlorophyll-deficient melon (Cucumis melo L.) mutants. We identified 390 differentially expressed proteins and 81 shared proteins varied significantly in abundance, of which 76 were upregulated and 5 were downregulated. Differentially expressed proteins were involved in the following biological processes: binding, catalytic, structural, transporter, and antioxidant activities. The mutant had 6.08-fold higher expression of glutamate-1-semialdehyde 2,1-aminomutase (GSAM), an enzyme that synthesizes the chlorophyll precursor, 5-aminolevulinic acid, and a 5.02-fold higher expression of pyridoxal biosynthesis protein, a GSAM coenzyme. An RNA recognition motif-containing protein (RRM) decreased in expression by 5.22-fold. This suggests that GSAM and RRM are particularly relevant to chlorophyll deficiency.

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Chloroplast pigments, proteins, lipid peroxidation and activities of antioxidative enzymes during maturation and senescence of leaves and reproductive organs of Cajanus cajan L.

Cited by 28 publications

References 40 publications

Study on the Anti-Aging Physiological Characteristics and Molecular Mechanism of Camellia Oleifera

Study on the Anti-Aging Physiological Characteristics and Molecular Mechanism of Camellia Oleifera

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

Proteomic analysis of chloroplasts from chlorophyll-deficient melon mutant

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