Chloroplast morphology and pyrenoid ultrastructural analyses reappraise the diversity of the lichen phycobiont genus Trebouxia (Chlorophyta)

Bordenave, César Daniel; Muggia, Lucía; Chiva, Salvador; Leavitt, Steven D.; Carrasco, Pedro; Barreno, Eva

doi:10.1016/j.algal.2021.102561

Cited by 24 publications

(31 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TEM was applied to study ultrastructural traits of pyrenoid and chloroplast both on the algae observed within the original thallus and on the corresponding axenically cultured strains. For morphological characterization of both pyrenoid and chloroplast types, we referred to the original classifications of Friedl (1989a , b) for pyrenoid types, that of Škaloud et al (2015) for the chloroplast types, and the recently compiled revision of pyrenoid and chloroplast types done by Bordenave et al (2022) . The following samples and strains were selected: Trebouxia “A52” thalli L2385, 2388, and 2389 and cultured strains L2906, L2908, L2912, and L2918; Trebouxia “A02” thalli L2421, L2732, and L2796 and cultured strains L2796, L3202, and L3015; T. vagua “A04” cultured strain L2943; and T. vagua “A10” cultured strain L2957.…”

Section: Methodsmentioning

confidence: 99%

“…Its species associate with mycobionts which are phylogenetically distantly related among Ascomycota and come from very diverse ecological conditions ( Nelsen et al, 2021 ). While Trebouxia has received considerable attention, only recently has species diversity been more fully recognized and characterized according to their genetic, and to a less extent morphological, diversity and ecological preferences ( Muggia et al, 2020 ; Nelsen et al, 2021 ; Bordenave et al, 2022 ). While 29 Trebouxia species have been formally described to date based on the combination of morphological traits and genetic diversity ( Friedl, 1989a , b ; Muggia et al, 2018 , 2020 ; Bordenave et al, 2022 ), the majority of species-level lineages in this important algal genus lack formal description.…”

Section: Introductionmentioning

confidence: 99%

“…Better characterization of diagnostic traits among distinct Trebouxia species-level lineages will be key to creating a robust, integrative taxonomy for this genus. Typical taxonomically diagnostic traits are based on the structure and the extension of the chloroplast lobes, and the distinct arrangement of thylakoid and osmiophilic pyrenoglobules in the pyrenoid ( Friedl, 1989a , b ; Muggia et al, 2014b , 2018 ; Molins et al, 2018a ; Bordenave et al, 2022 ). To perform reliable analyses of these traits, axenic cultures of Trebouxia phycobionts are essential.…”

Section: Introductionmentioning

confidence: 99%

“…Culturing algal cells is also important to correlate the in vitro traits with those exhibited in the symbiotic state inside the lichen thalli. Bordenave et al (2022) recently compiled a new morphological and ultrastructural characterization of 20 Trebouxia species-level lineages, reappraising and implementing the classification of Trebouxia in accordance with the phylogenetic delimitation provided by Muggia et al (2020) .…”

Section: Introductionmentioning

confidence: 99%

“…We hypothesize that R. melanophthalma and T. atra collected at high elevation and in very dry conditions share Trebouxia photobionts unique to this type of habitats, while we expect to recover already-identified species-level lineages from samples at lower altitude and less selective environmental conditions. The works of Muggia et al (2020) and Bordenave et al (2022) are here used as references for naming the species-level lineages and characterizing their phenotypic traits, respectively.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Photobiont Diversity in Lichen Symbioses From Extreme Environments

et al. 2022

Self Cite

View full text Add to dashboard Cite

Fungal–algal relationships—both across evolutionary and ecological scales—are finely modulated by the presence of the symbionts in the environments and by the degree of selectivity and specificity that either symbiont develop reciprocally. In lichens, the green algal genus Trebouxia Puymaly is one of the most frequently recovered chlorobionts. Trebouxia species-level lineages have been recognized on the basis of their morphological and phylogenetic diversity, while their ecological preferences and distribution are still only partially unknown. We selected two cosmopolitan species complexes of lichen-forming fungi as reference models, i.e., Rhizoplaca melanophthalma and Tephromela atra, to investigate the diversity of their associated Trebouxia spp. in montane habitats across their distributional range worldwide. The greatest diversity of Trebouxia species-level lineages was recovered in the altitudinal range 1,000–2,500 m a.s.l. A total of 10 distinct Trebouxia species-level lineages were found to associate with either mycobiont, for which new photobionts are reported. One previously unrecognized Trebouxia species-level lineage was identified and is here provisionally named Trebouxia “A52.” Analyses of cell morphology and ultrastructure were performed on axenically isolated strains to fully characterize the new Trebouxia “A52” and three other previously recognized lineages, i.e., Trebouxia “A02,” T. vagua “A04,” and T. vagua “A10,” which were successfully isolated in culture during this study. The species-level diversity of Trebouxia associating with the two lichen-forming fungi in extreme habitats helps elucidate the evolutionary pathways that this lichen photobiont genus traversed to occupy varied climatic and vegetative regimes.

show abstract

Section: Methodsmentioning

confidence: 99%