Chloroplast membranes of the green alga Acetabularia mediterranea. II. Topography of the chloroplast membrane.

Apel, Klaus; Miller, Kenneth R.; Bogorad, Lawrence; Miller, G. J.

doi:10.1083/jcb.71.3.876

Cited by 19 publications

(7 citation statements)

References 31 publications

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“…Moreover, many polypeptides are extracted specifically, which produces membranes with a polypeptide composition simpler than that of untreated thylakoids. This confirms previous results obtained with Vicia faba and Acetabularia (1,10,16). Because membrane structure is preserved, as judged by thin-sectioning and freeze-fracturing, it is possible to draw certain conclusions with respect to the location of polypeptides within the thylakoids, and to make correlations between polypeptide composition and freeze-fracture particles.…”

Section: Discussionsupporting

confidence: 82%

Correlation between the freeze fracture appearance and polypeptide composition of thylakoid membranes in barley

Machold

Simpson

Høyer‐Hansen

1977

Carlsberg Res. Commun.

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Thylakoids isolated and purified from wild-type barley chloroplasts were treated to remove polypeptides without destroying the membrane structure. The polypeptide compositions of such basic membranes were determined by SDS-polyacrylamide gel electrophoresis and the ultrastructure of the membranes determined in thin sections of fixed material and by freeze-fracturing. Treatments with guanidine hydrochloride, trypsin and subtilisin removed most of the polypeptides except for the chlorophyll-protein complexes. Freeze-fracture particles were observed in the basic membranes, although the size distribution and number per unit area were altered. It is concluded that the chlorophyll-protein complexes are the major components of the freeze-fracture particles present after treatment, and that most of the thylakoid polypeptides are susceptible to proteolytic attack at the membrane surface. The only extraction procedure that removed both chlorophyll-protein complexes (6 M-guanidine thiocyanate) also disintegrated the membranes, resulting in lipid artifact structures. Thylakoids isolated from the chlorina-f2 mutant were more susceptible to disintegration when treated in the same way as wild-type, possibly due to the absence of chlorophyll-protein complex II.

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Section: Discussionsupporting

confidence: 82%

Correlation between the freeze fracture appearance and polypeptide composition of thylakoid membranes in barley

Machold

Simpson

Høyer‐Hansen

1977

Carlsberg Res. Commun.

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“…Groups of proteins also have been identified as LHC subunits in Acetabularia (1), Chlamydomonas (2), lettuce (17), and pea seedlings (9). Some proteins which should perhaps be included in this second group are those which are only barely detectable in etioplasts and which seem to increase in amount during greening, relative to other proteins also present in etioplasts.…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, improved techniques for electrophoretic separations of membrane proteins have been employed both to 1 This work was supported in part by the National Institute of General Medical Sciences Grant GM-20470 (8,12,14,25) on the basis of their sizes. We have employed a discontinuous buffer system, which provides stacking of sample proteins, and a polyacrylamide gradient in the running gel to improve resolution of proteins over a wide range of mol wt further.…”

mentioning

confidence: 99%

Comparison of the Molecular Weights of Proteins Synthesized by Isolated Chloroplasts with Those Which Appear during Greening in Zea mays

Grebanier

Steinback²,

Bogorad³

1979

Plant Physiol.

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(8,12,14,25) on the basis of their sizes. We have employed a discontinuous buffer system, which provides stacking of sample proteins, and a polyacrylamide gradient in the running gel to improve resolution of proteins over a wide range of mol wt further.Fluorography of the SDS-polyacrylamide gels was used to determine the number and mol wt of the membrane-associated products synthesized during incubation ofchloroplasts with radioactive amino acids. It is more difficult to quantitate amounts of radioactivity with fluorography than by slicing the gels and counting the radioactivity in each slice. However, fluorography offers the advantages of higher resolution of minor bands and greater precision in aligning peaks of radioactivity with stained proteins on the same gel, making it the most useful technique available for the kinds of comparisons we wished to make. MATERIALS AND METHODSPlant Material. Seeds of Zea mays (FR9CMS x FR37, Illinois Foundation Seeds) were soaked for 20 h, then grown in Vermiculite, either in a dark room or in a greenhouse. Plants kept in the dark room were exposed only to brief periods of green light during watering and handling. They were used 7 to 9 days after planting. For greening of plants, the trays were removed to a room with full lighting, i.e. about 90 to 120 ft-c and room temperature.Chloroplast

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“…PS II vesicles of varying purity have been isolated from algae, cyanobacteria and higher plants but these have all been inactive with respect to H20-splitting activity (10,30,39). Their photochemical characterization was therefore based on measurements using the artificial electron donor diphenylcarbazide.…”

Section: Discussionmentioning

confidence: 99%

Polypeptide composition of an oxygen evolving photosystem II vesicle from spinach chloroplasts

Henry

Møller

1981

Carlsberg Res. Commun.

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Chloroplast membranes of the green alga Acetabularia mediterranea. II. Topography of the chloroplast membrane.

Abstract: The localization of the chlorophyll-protein complexes inside the thylakoid membrane of Acetabularia rnediterranea was determined by fractionating the chloroplast membrane with EDTA and Triton X-100, by using pronase treatment, and by labeling the surface-exposed proteins with 1251.

Cited by 19 publications

References 31 publications

Correlation between the freeze fracture appearance and polypeptide composition of thylakoid membranes in barley

Correlation between the freeze fracture appearance and polypeptide composition of thylakoid membranes in barley

Comparison of the Molecular Weights of Proteins Synthesized by Isolated Chloroplasts with Those Which Appear during Greening in Zea mays

Polypeptide composition of an oxygen evolving photosystem II vesicle from spinach chloroplasts

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