Chloroplast Genomics for Sustainable Cotton Production

Ahmad, Niaz; Wei, Zhengyi; Khan, Muhammad Sarwar; Nielsen, Brent L.

doi:10.1007/978-3-030-64504-5_3

Cited by 1 publication

(1 citation statement)

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“…In short, the promoter, UTRs or expression techniques are not the only factors affecting rate of expression. Random integration in nucleus as well as in chloroplast integration site overall affect the expression of transgene (Ahmad et al 2021).…”

Section: Promoter Selection and Codon Optimizationmentioning

confidence: 99%

Transformation and Expression of Gene in Microalgae: From Tools to Applications

Usmaan¹,

Rao²

2021

Agrobiol. Records

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Microalgae provides an excellent platform for foreign genes expression. Chloroplast and Nucleus based transformation of transgenes in algae is possible after construct designing (including different markers and promoters) and codon optimization. For better recombination, different and effective promoters and reporter genes can be selected. Chloroplast mediated transformation is much more effective than nuclear based transformation for transgene analysis owing to higher copy number of chloroplast in unit area. Microalgae based products are excellent target for production of wide variety of bio products such as vaccines, antibiotics and drugs against different diseases. These alternative approaches facilitate enhanced biodiesel production through fast growth of microalgae in heterotrophic environment. Also, low risks are attributed owing to their nonhuman consumption property. Biosafety and risk assessment analysis should be carried out before releasing of genetically modified microalgae in the environment considering research ethical code. Microalgae provide cheap, efficient and high yielding alternatives to plants. The review emphasizes microalgae based expression approach with key steps involved in detail.

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Section: Promoter Selection and Codon Optimizationmentioning

confidence: 99%