Chloroplast Genetic Engineering: Recent Advances and Future Perspectives

Grevich, Justin James; Daniell, Henry

doi:10.1080/07352680590935387

Cited by 102 publications

(72 citation statements)

References 143 publications

(162 reference statements)

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“…Since only plant-derived sequences are used for gene integration and transgenic ptDNA selection, an additional advantage derives from the fact that the removal of undesired heterologous sequences (e.g. antibiotic resistance genes) is not required following the isolation of homoplasmic transformants (Grevich and Daniell 2005;Lutz and Maliga 2007). On the other hand, to achieve transformation efficiencies comparable to those attainable by biolistic delivery of ''inactivatingtype'' vectors, the ''binding-type'' approach requires an efficient protoplast-to-plant regeneration system, thus restricting the range of species to which it can be applied.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, to demonstrate the feasibility of using the ''binding-type'' vector strategy for manipulating the expression of agronomically relevant genes, the two D 9 desaturase genes were cloned in vectors containing only plant-derived sequences and inserted in transgenic plastids by PEG transformation of plant protoplasts. In terms of biosafety, besides the drastic reduction in the possibility of transgene flow into the wider environment presented by transplastomic plants, the latter vectors reliance on antibiotic insensitivity means that further manipulation for the removal of marker gene(s) is obviated (Grevich and Daniell 2005;Lutz and Maliga 2007).…”

Section: Introductionmentioning

confidence: 99%

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Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Craig¹,

Lenzi²,

Scotti³

et al. 2008

Transgenic Res

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The possibility of altering the unsaturation level of fatty acids in plant lipids by genetic transformation has implications for the stress tolerance of higher plants as well as for their nutritional value and industrial utilisation. While the integration and expression of transgenes in the plastome has several potential advantages over nuclear transformation, very few attempts have been made to manipulate fatty acid biosynthesis using plastid transformation. We produced transplastomic tobacco plants that express a D 9 desaturase gene from either the wild potato species Solanum commersonii or the cyanobacterium Anacystis nidulans, using PEG-mediated DNA uptake by protoplasts. Incorporation of chloroplast antibioticinsensitive point mutations in the transforming DNA was used to select transformants. The presence of the transcript and the D 9 desaturase protein in transplastomic plants was confirmed by northern and western blot analyses. In comparison with control plants, transplastomic plants showed altered fatty acid profiles and an increase in their unsaturation level both in leaves and seeds. The two transgenes produced comparable results. The results obtained demonstrate the feasibility of using plastid transformation to engineer lipid metabolic pathways in both vegetative and reproductive tissues and suggest an increase of cold tolerance in transplastomic plants showing altered leaf fatty acid profiles. This is the first example of transplastomic plants expressing an agronomically relevant gene produced with the ''binding-type'' vectors, which do not contain a heterologous marker gene. In fact, the transplastomic plants expressing the S. commersonii gene contain only plant-derived sequences, a clear attraction from a public acceptability perspective.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Craig¹,

Lenzi²,

Scotti³

et al. 2008

Transgenic Res

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show abstract

“…La production de protéines recombinantes peut également avoir lieu dans les chloroplastes. Ces organites, comme les bactéries, sont parfaitement adaptées à la production en très grande quantité de molécules thérapeutiques simples [7].…”

Section: Diversité Des Systèmes De Production De Médicaments Recombinunclassified

Plantes, médicaments et génétique

Faye

Champey

2008

Med Sci (Paris)

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“…It was not only the highest accumulation of protein in transgenic plants but a complete bacterial operon was successfully expressed, for the first time, resulting in the formation of stable cry2Aa2 crystals. Transgenes express large amounts of foreign protein due to the high copy number of the chloroplast genome in each plant cell-up to 10,000 copies of transgene per cell [2][3][4][5][6][7][8]. Transgenes are integrated into the spacer regions of the chloroplast genome by homologous recombination of flanking sequences.…”

mentioning

confidence: 99%