Chloroplast Genes for Photosynthetic Membrane Components

Gray, John C.; Blyden, E. Richard; Eccles, Christopher J.; Dunn, P. P. J.; Hird, Sean M.; Höglund, Anders; Kaethner, T. M.; Smith, Alison G.; Willey, David L.; Dyer, Tristan A.

doi:10.1007/978-94-017-0519-6_128

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…If this view is correct it suggests that the chloroplast genome encodes all [29] and barley [17] proteins are compared with amino acid sequences deduced from the liverwortfrxA gene [30] and the psaC gene of tobacco [15], wheat and pea. [4,14].…”

Section: Characteristics Of the 8 Kda Polypeptidementioning

confidence: 99%

Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA

Dunn

Gray

1988

Plant Mol Biol

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The gene for the 8 kDa subunit of photosystem I has been located in the small single copy region of wheat chloroplast DNA by coupled transcription-translation of cloned fragments of DNA and by DNA sequence analysis. The pea gene for this subunit was located in pea chloroplast DNA by using the wheat gene as a probe. The location was confirmed by immunoprecipitation of the products of coupled transcription-translation of cloned DNA with antiserum raised against the small subunits of pea photosystem I and by DNA sequence analysis. The deduced amino acid sequences of the pea and wheat proteins are identical in seventy-six out of the eighty-one amino acid residues. There are nine conserved cysteine residues, eight of which are arranged in the primary structure in a similar way to those in bacterial ferredoxins containing two 4Fe-4S centres, suggesting that the polypeptide binds iron sulphur centres A and B of photosystem I.

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Section: Characteristics Of the 8 Kda Polypeptidementioning

confidence: 99%

Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA

Dunn

Gray

1988

Plant Mol Biol

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Nucleotide sequence of the genes encoding cytochrome b-559 from the cyanelle genome of Cyanophora paradoxa

Cantrell

Bryant

1988

Molecular Biology of Photosynthesis

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Nucleotide sequence of the genes encoding cytochrome b-559 from the cyanelle genome of Cyanophora paradoxa

Cantrell

Bryant

1988

Photosynth Res

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Cyanophora paradoxa is a flagellated protozoan which possesses unusual, chloroplast-like organelles referred to as cyanelles. The psbE and psbF genes, which encode the two apoprotein subunits of cytochrome b-559, have been cloned from the cyanelle genome of C. paradoxa. The complete nucleotide sequences of these genes and their flanking sequences were determined by the chain-termination, dideoxy method. The psbE gene is composed of 75 codons and predicts a polypeptide of 8462 Da that is seven to nine residues smaller than most other psbE gene products. The psbF gene consists of 43 codons and predicts a polypeptide of 4761 Da. Two open reading frames, whose sequences are highly conserved among cyanobacteria and numerous higher plants, were located in the nucleotide sequence downstream from the psbF gene. The first open reading frame, denoted psbI, is composed of 39 codons, while the second open reading frame, denoted psbJ, is composed of 41 codons. The predicted amino acid sequences of the psbI and psbJ gene products predict proteins of 5473 and 3973 Da respectively. These proteins are probably integral membrane proteins anchored in the membrane by a single, transmembrane alpha helix. The psbEFIJ genes are probably co-transcribed and constitute an operon as found for other organisms. Each of the four genes is preceded by a polypurine sequence which resembles the consensus ribsosome binding sequences for Escherichia coli.

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Chloroplast Genes for Photosynthetic Membrane Components

Cited by 4 publications

References 29 publications

Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA

Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA

Nucleotide sequence of the genes encoding cytochrome b-559 from the cyanelle genome of Cyanophora paradoxa

Nucleotide sequence of the genes encoding cytochrome b-559 from the cyanelle genome of Cyanophora paradoxa

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