Chloroplast-generated ROS dominate NaCl<sup>-</sup>induced K<sup>+</sup>efflux in wheat leaf mesophyll

Wu, Honghong; Shabala, Lana; Zhou, Meixue; Shabala, Sergey

doi:10.1080/15592324.2015.1013793

Cited by 27 publications

(15 citation statements)

References 38 publications

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“…The enzyme has been functionally characterized as involved in the biosynthesis of the heme of photosynthetic cytochromes and stress response proteins [ 37 ]. Our data are in accordance with previous studies that showed that stress induced by salinity and water stress result in upregulated genes of ferrochelatase [ 38 , 39 , 40 ].…”

Section: Discussionsupporting

confidence: 93%

Response of Downy Oak (Quercus pubescens Willd.) to Climate Change: Transcriptome Assembly, Differential Gene Analysis and Targeted Metabolomics

Mévy

Loriod

Liu

et al. 2020

Plants

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Global change scenarios in the Mediterranean basin predict a precipitation reduction within the coming hundred years. Therefore, increased drought will affect forests both in terms of adaptive ecology and ecosystemic services. However, how vegetation might adapt to drought is poorly understood. In this report, four years of climate change was simulated by excluding 35% of precipitation above a downy oak forest. RNASeq data allowed us to assemble a genome-guided transcriptome. This led to the identification of differentially expressed features, which was supported by the characterization of target metabolites using a metabolomics approach. We provided 2.5 Tb of RNASeq data and the assembly of the first genome guided transcriptome of Quercus pubescens. Up to 5724 differentially expressed transcripts were obtained; 42 involved in plant response to drought. Transcript set enrichment analysis showed that drought induces an increase in oxidative pressure that is mitigated by the upregulation of ubiquitin-like protein protease, ferrochelatase, oxaloacetate decarboxylase and oxo-acid-lyase activities. Furthermore, the downregulation of auxin biosynthesis and transport, carbohydrate storage metabolism were observed as well as the concomitant accumulation of metabolites, such as oxalic acid, malate and isocitrate. Our data suggest that early metabolic changes in the resistance of Q. pubescens to drought involve a tricarboxylic acid (TCA) cycle shunt through the glyoxylate pathway, galactose metabolism by reducing carbohydrate storage and increased proteolytic activity.

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Section: Discussionsupporting

confidence: 93%

Response of Downy Oak (Quercus pubescens Willd.) to Climate Change: Transcriptome Assembly, Differential Gene Analysis and Targeted Metabolomics

Mévy

Loriod

Liu

et al. 2020

Plants

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“…Influx of Na + worsens the K/Na ratio, and this is further exacerbated by salt stress-induced K + loss, a phenomenon that is often more pronounced in salt-sensitive species (Chen et al., 2007; Wu et al., 2018). GORK (guard cell outward rectifying K + channel) type and ROS-activated NSCC-type channels are likely to mediate the main fraction of K + efflux (Jayakannan et al., 2013; Wu et al., 2015b). In addition, salt stress-induced K + leakage can cause PCD (Figure 2; Demidchik et al., 2014).…”

Section: What Is the Role Of Potassium In Salinity Stress?mentioning

confidence: 99%

Plant Salinity Stress: Many Unanswered Questions Remain

2019

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Salinity is a major threat to modern agriculture causing inhibition and impairment of crop growth and development. Here, we not only review recent advances in salinity stress research in plants but also revisit some basic perennial questions that still remain unanswered. In this review, we analyze the physiological, biochemical, and molecular aspects of Na + and Cl − uptake, sequestration, and transport associated with salinity. We discuss the role and importance of symplastic versus apoplastic pathways for ion uptake and critically evaluate the role of different types of membrane transporters in Na + and Cl − uptake and intercellular and intracellular ion distribution. Our incomplete knowledge regarding possible mechanisms of salinity sensing by plants is evaluated. Furthermore, a critical evaluation of the mechanisms of ion toxicity leads us to believe that, in contrast to currently held ideas, toxicity only plays a minor role in the cytosol and may be more prevalent in the vacuole. Lastly, the multiple roles of K + in plant salinity stress are discussed.

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“…Moreover, salicylic acid can also participate in this reaction but via peroxidase-dependent ROS production, resulting in very similar Ca 2+ influx-mediated phenomena (reviewed by Sierla et al [ 56 ]). Intriguingly, Wu et al [ 57 ] have recently shown that leaf mesophyll ROS production for activation of K + efflux can be catalyzed by disruption of electron flow in the chloroplast electron transport chains. This suggests a different pattern of regulation comparing to the guard cells.…”

Section: Ca 2+ Influx Activated By H mentioning

confidence: 99%

“…Similar to root hairs, a steep Ca 2+ activity gradient in the tip of elongating pollen tubes is regulated via ROS produced by the NADPH oxidase [ 87 ]. Wu et al [ 57 ] demonstrated that exogenous H 2 O 2 rapidly activated plasma membrane inwardly-rectifying Ca 2+ -permeable conductance in pear pollen tube protoplasts. Breygina et al [ 88 ] found very similar H 2 O 2 -activated conductances in lily pollen grain protoplasts.…”

Section: Pollen Tube Ros-activated Channelsmentioning

confidence: 99%

ROS-Activated Ion Channels in Plants: Biophysical Characteristics, Physiological Functions and Molecular Nature

Demidchik

2018

IJMS

119

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Ion channels activated by reactive oxygen species (ROS) have been found in the plasma membrane of charophyte Nitella flixilis, dicotyledon Arabidopsis thaliana, Pyrus pyrifolia and Pisum sativum, and the monocotyledon Lilium longiflorum. Their activities have been reported in charophyte giant internodes, root trichoblasts and atrichoblasts, pollen tubes, and guard cells. Hydrogen peroxide and hydroxyl radicals are major activating species for these channels. Plant ROS-activated ion channels include inwardly-rectifying, outwardly-rectifying, and voltage-independent groups. The inwardly-rectifying ROS-activated ion channels mediate Ca2+-influx for growth and development in roots and pollen tubes. The outwardly-rectifying group facilitates K+ efflux for the regulation of osmotic pressure in guard cells, induction of programmed cell death, and autophagy in roots. The voltage-independent group mediates both Ca2+ influx and K+ efflux. Most studies suggest that ROS-activated channels are non-selective cation channels. Single-channel studies revealed activation of 14.5-pS Ca2+ influx and 16-pS K+ efflux unitary conductances in response to ROS. The molecular nature of ROS-activated Ca2+ influx channels remains poorly understood, although annexins and cyclic nucleotide-gated channels have been proposed for this role. The ROS-activated K+ channels have recently been identified as products of Stellar K+ Outward Rectifier (SKOR) and Guard cell Outwardly Rectifying K+ channel (GORK) genes.

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Chloroplast-generated ROS dominate NaCl^-induced K⁺efflux in wheat leaf mesophyll

Cited by 27 publications

References 38 publications

Response of Downy Oak (Quercus pubescens Willd.) to Climate Change: Transcriptome Assembly, Differential Gene Analysis and Targeted Metabolomics

Response of Downy Oak (Quercus pubescens Willd.) to Climate Change: Transcriptome Assembly, Differential Gene Analysis and Targeted Metabolomics

Plant Salinity Stress: Many Unanswered Questions Remain

ROS-Activated Ion Channels in Plants: Biophysical Characteristics, Physiological Functions and Molecular Nature

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Chloroplast-generated ROS dominate NaCl-induced K+efflux in wheat leaf mesophyll

Cited by 27 publications

References 38 publications

Response of Downy Oak (Quercus pubescens Willd.) to Climate Change: Transcriptome Assembly, Differential Gene Analysis and Targeted Metabolomics

Response of Downy Oak (Quercus pubescens Willd.) to Climate Change: Transcriptome Assembly, Differential Gene Analysis and Targeted Metabolomics

Plant Salinity Stress: Many Unanswered Questions Remain

ROS-Activated Ion Channels in Plants: Biophysical Characteristics, Physiological Functions and Molecular Nature

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Chloroplast-generated ROS dominate NaCl^-induced K⁺efflux in wheat leaf mesophyll