Chloroplast DNA polymorphism in different types of cytoplasmic male sterile rice

Abstract

The lengths of open reading frame (ORF)100 and ORF29-TrnC GCA , the intronic sequence of rps16 and the transcribed spacer of TrnT UGU -TrnL UAA in chloroplast from different lines of cytoplasmic male sterility (CMS) rice were studied using indica types, japonica types and common wild rice as controls. The results show that the lengths of ORF100 and ORF29-TrnC GCA in CMS lines are similar to those of typical indica. The sequences of the rps16 intron and the TrnT UGU -TrnL UAA spacer in sporophyte sterile types (wild-abortive type, Yinshui type and K type) are almost the same, and they also share a molecular marker of GTTGAG at nucleotide positions 220 -225 in the rps16 intron. Therefore, it is speculated that the source of these three types is the same. In contrast, a gametophyte sterile type, Yuetai A does not contain such a GTTGAG sequence in the rps16 intron and has a unique G at position 595, which may works as a molecular marker distinguishing the sporophyte sterile type from the gametophyte sterile type. Based on the observation that CMS rice has much lower cytoplasmic polymorphism than indica, japonica and wild rice, it is concluded that CMS rice lack cytoplasm diversity. Therefore, it is important to introduce new sources of cytoplasm into hybrid rice.

Chloroplast Genes Are Involved in The Male-Sterility of K-Type CMS in Wheat

“…Additionally, it was found that the matK gene appeared three times in different primers of PCR amplification, and the amplification bands were enhanced in abortive buds, which showed upregulated expression, indicating that the abortion of radish buds had a great relationship with matK gene. Ou et al [ 77 ] studied the intron sequences of ORFs and rps16 genes in chloroplasts of different CMS lines in rice. Additionally, they found that in the gametophyte sterility line, the rps16 gene intron contains a GMTGAG sequence and a unique G at position 595, which can be used as a molecular marker to distinguish sporophytic sterility and gametophyte sterility.…”

Assembly and comparative analysis of chloroplast genome of wheat K-CMS line and maintainer line

“…And it was found that the matK gene appeared three times in different primers of PCR amplification, and the amplification bands were enhanced in abortive buds, which showed upregulated expression, indicating that the abortion of radish buds had a great relationship with matK gene. Ou et al [71] studied the intron sequences of ORFs and rps16 genes in chloroplasts of different CMS lines in rice. And they found that in the gametophyte sterility line, the rps16 gene intron contains a GMTGAG sequence and a unique G at position 595, which can be used as a molecular marker to distinguish sporophytic sterility and gametophyte sterility.…”

Chloroplast-specific universal primers and their uses in plant studies

“…Prentice et al (2003) implied chloroplast universal primers to study variation in cultivated and wild Prunus avium. Other examples for the use of universal primers to assess intraspecific variation are those carried out on Argania spinosa (Sapotaceae) of Morocco (El Mousadik and Petit 1996), Primula cuneifolia (Primulaceae) (Fujii et al 1999), Cannabis sativa (Kohjyouma et al 2000), the rare Spanish endemic, Silene hifacensis (Prentice et al 2003), Sagittaria latifolia (Dorken and Barrett 2004), and Oryza sativa (Ou et al 2009). …”