ABSTRACI Pigment-protein complexes were isolated from chloroplasts of normal green and several types of chlorophyll-deficient soybeans. The complexes were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and comparisons were made between normal and chlorophylldeficient genotypes of the relative amounts of chlorophyll associated with Photosystem I (PSI), Photosystem II (PSII1 light-harvesfing, and free pigment complexes.Chlorophyll-deficient genotypes, compared to normal green genotypes, have fewer light-harvesting complexes and a higher ratio of PSII to PSI complexes. Chlorophyll associated with PSII in yellow genotypes is in relatively higher amounts in spite of the fact that these genotypes have much less grana stacking than normal green genotypes. Although PSII activity has been associated with appressed regions of grana in normal plants, our work shows that the association does not always hold true.Our research in recent years has been concerned with elucidation of pigment changes in the chloroplasts of various soybean genotypes which exhibit Chl deficiency during growth and development (5, 8). These mutants are backcrossed-developed isolines with single gene differences for pigment deficiency. That is, spontaneous mutants have been backcrossed to a parent line (Clark) through at least five generations of progeny (4). We have chosen to work with the parent, Clark L,, and three isoline mutants, Clark y3y3, Clark y9yg, and Clark Y IyI 1. Compared to the parent line, CLI, the mutant Cy3y3 is only slightly pigmentdeficient in early development but exhibits premature senescence which is strongly activated by seed set and pod filling. Cygyg shows early and pronounced pigment-deficiency that lasts until near the end of the growing season. CY, ly, 1, which is studied as the heterozygote because the doubly recessive y1I gene is lethal, is very similar in pigment deficiency to Cygyg, but its maximum deficiency is expressed later in plant development and does not recover greenness in late season as much as Cy9y9 does. Because of the broad range of pigment-deficiencies represented by these genotypes, they are quite useful in studying the chloroplast in its various stages ofdevelopment (5). In the past (9), we have shown that pigment analysis of these various genotypes may be used to suggest the course ofpigment-protein development during greening. Now, using gel electrophoresis (SDS-PAGE2) we have exam- 'The mention of firm names or trade products does not imply that they are endorsed or recommended by the United States Department of Agriculture over other firms or similar products not mentioned.2 Abbreviations: PAGE, polyacrylamide gel electrophoresis; LHPP/ LHCP, light-harvesting pigment-protein(s). ined these same soybean genotypes for pigment-proteins during various stages of pigment deficiency. Although the number and type of bands that are obtained by electrophoretic methods may vary with the technique, it is generally accepted that the major bands are: CPla and CP1 (the reaction centers of P...