Chlorophyll Supplementation in Early Life Prevents Diet‐Induced Obesity and Modulates Gut Microbiota in Mice

Abstract

Scope: The high rates of overweight and obesity, currently occurring in children, underline the urgent need for preventive strategies in early life before excess weight is gained. Methods and results: In this study, the alleviating obesity property of chlorophyll supplementation in early life is investigated using a 4-week-old C57BL/6J male mice model with obesity induced by high-fat diet (HFD). The present study finds that chlorophyll supplementation in early life can effectively retard body weight gain, improve glucose tolerance, as well as reduce low-grade inflammation in HFD-fed mice. Consequently, high-throughput sequencing of the 16S rRNA demonstrates that chlorophyll supplementation significantly reversed the HFD-induced gut dysbiosis, as evidenced by the decreased Firmicutes-to-Bacteroidetes ratios as chlorophyll is introduced. Furthermore, in chlorophyll-fed mice, the increased abundance of Blautia and norank f Bacteroidales S24-7 group, and the decreased abundance of Lactococcus and Lactobacillus, bring about the possibility that chlorophyll's alterations to the gut microbiota composition would be the main factors for dietary chlorophyll alleviating obesity-related indexes. Conclusion: Taken together, these results reveal that the intake of chlorophyll in early life may propel healthy weight management and produce beneficial effects against potential obesity in later life.

Protective effects of selenium-enriched peptides from Cardamine violifolia against high-fat diet induced obesity and its associated metabolic disorders in mice

“…It could be improved by the intervention of prebiotic and some plant compounds, which contributed to the alleviation of inammation, insulin resistance, and obesity. 34,36 Consistent with the previous studies, the protective effect of CSP on diet-induced obesity might be via the promotion of this benecial gut bacteria. Moreover, a distinct increase in Lactobacillus, which is also positively correlated with obesity, was independently discovered in the CSPH groups.…”

Metabolomic signatures for liver tissue and cecum contents in high-fat diet-induced obese mice based on UHPLC-Q-TOF/MS

“…Most studies used male mice to construct high-fat diet-induced obesity model [ 12 , 65 , 66 ]. In the study, we used female mice in order to discover different outcomes in males based on their different hormonal profile.…”

The Marine Microalga, Tisochrysis lutea, Protects against Metabolic Disorders Associated with Metabolic Syndrome and Obesity

“…A previous animal study demonstrated that chlorophyll supplementation (0.18 mg/10 g body weight/day) could also exert beneficial effects against obesity by the decrease of body weight gain, the improvement of glucose tolerance and the reduction of low-grade inflammation in HFD-fed C57BL/6 J male mice [ 50 ]. These effects could be due to the preventive action of chlorophyll supplementation on gut dysbiosis, characterized by the decreased Firmicutes/Bacteroidetes ratios, the increased abundance of Blautia bacteria, and the significant decrease of Lactococcus and Lactobacillus bacteria [ 50 ].…”