Chlorophyll retention caused by STAY-GREEN (SGR) gene mutation enhances photosynthetic efficiency and yield in soybean hybrid Z1

Wang, P.; Hou, Sen; Wen, Hu; Wang, Q.Z.; Li, G.Q.

doi:10.32615/ps.2020.076

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…The dry weight of 100 seeds of ZX12 was higher than that obtained for ZX4, indicating that the late-senescent phenotype of ZX12 is not due to interruption of the Chl degradation process ( Figure S1C ). The soybean delayed senescence mutant z1 had a lower leaf Chl degradation rate and higher photosynthetic efficiency during maturation than the control varieties, consistent with the phenotype of ZX12 in this study [ 62 ]. For yield-related characteristics, although z1 had a higher seed mass per plant, the 100-seed weight of z1 was lower than that of control varieties, which was inconsistent with the result that ZX12 had a higher 100-grain dry weight than ZX4.…”

Section: Discussionsupporting

confidence: 84%

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Wang

Chen

et al. 2022

Antioxidants

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(1) Background: Senescence represents the final stage of plant growth and development, which transfers nutrients to growing seeds and directly affects the yield and quality of crops. However, little is known about chlorophyll degradation in developing and maturing seeds, in contrast to leaf senescence; (2) Methods: RNA-Seq was used to analyze the differentially expressed genes of different late-senescent germplasms. A widely untargeted metabolic analysis was used to analyze differential metabolites. In addition, qRT-PCR was conducted to detect gene expression levels; (3) Results: Transcriptome analysis revealed that ZX12 seeds have a higher expression level of the chlorophyll synthesis genes in the early stage of maturity, compared with ZX4, and have a lower expression level of chlorophyll degradation genes in the late stage of maturity. Flavonoids were the primary differential metabolites, and ZX12 contains the unique and highest expression of three types of metabolites, including farrerol-7-O-glucoside, cyanidin-3-o-(6′-o-feruloyl) glucoside, and kaempferide-3-o-(6′-malonyl) glucoside. Among them, farrerol-7-O-glucoside and cyanidin-3-o-(6′-o-feruloyl) glucoside are flavonoid derivatives containing mono and dihydroxy-B-ring chemical structures, respectively; and (4) Conclusions: It is speculated that the two metabolites can slow down the degradation process of chlorophyll by scavenging oxygen-free radicals in the chloroplast.

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Section: Discussionsupporting

confidence: 84%

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Wang

Chen

et al. 2022

Antioxidants

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“…However, functional stay-green mutants might be helpful to further increase crop yields because they can maintain photosynthesis during the later stages of growth, thus leading to the accumulation of increased amounts of photosynthetic products (Luo et al, 2006;Wang et al, 2021). Various studies have suggested that the cosmetic stay-green phenotype usually results from mutations in genes involved in leaf senescence, photosynthesis, chlorophyll (Chl) metabolism and antioxidative enzyme activity (Kura-Hotta et al, 1987;Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars

Yang

Guo

et al. 2022

Front. Plant Sci.

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Breeders agree that leaf senescence is a favorable process for wheat seed yield improvement due to the remobilization of leaf nutrients. However, several studies have suggested that staying green may be an important strategy for further increasing wheat yields. In this study, we performed a comparative transcriptome analysis between wheat cultivars CN17 and CN19 after heading and also measured photosynthetic parameters, chlorophyll (Chl) contents, and antioxidant enzyme activities at various time points after heading. The physiological and biochemical indexes revealed that CN17 exhibited a functionally stay-green phenotype while CN19 did not. We identified a total of 24,585 and 34,410 differential expression genes between genotypes at two time-points and between time-points in two genotypes, respectively, and we also found that 3 (37.5%) genes for leaf senescence, 46 (100%) for photosynthesis – antenna protein, 33 (70.21%) for Chl metabolism and 34 (68%) for antioxidative enzyme activity were upregulated in CN17 compared with CN19 during leaf senescence, which could be regulated by the differential expression of SAG39 (senescence-associated gene 39), while 22 (100%) genes for photosynthesis – antenna proteins, 6 (46.15%) for Chl metabolism and 12 (80%) for antioxidative enzyme activity were upregulated in CN17 compared with CN19 before the onset of leaf senescence. Here, we further clarified the expression profiles of genes associated with a functional stay-green phenotype. This information provides new insight into the mechanism underlying delayed leaf senescence and a new strategy for breeders to improve wheat yields.

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“…For example, the ‘stay-green’ genotype GGd1d1d2d2 increased seed yield in growth chamber conditions but reduced yield outdoors compared to its near-isogenic line wild type ‘Clark’ 58 . In contrast, a double mutant of SGR1 and SGR2 , which is a typical ‘stay-green’ variety (Z1), showed a higher yield performance compared to its parents JD74 59 . Here, we showed that knocking out GmWRKY100 can increase yield per plant by more than 10%, potentially by increasing the rate of photosynthesis and extending the photosynthetically functional phase without obvious developmental defects.…”

Section: Discussionmentioning

confidence: 93%

The mechanism of low blue light-induced leaf senescence mediated by GmCRY1s in soybean

Li,

Lyu,

et al. 2024

Nat Commun

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Leaf senescence is a crucial trait that has a significant impact on crop quality and yield. Previous studies have demonstrated that light is a key factor in modulating the senescence process. However, the precise mechanism by which plants sense light and control senescence remains largely unknown, particularly in crop species. In this study, we reveal that the reduction in blue light under shading conditions can efficiently induce leaf senescence in soybean. The blue light receptors GmCRY1s rather than GmCRY2s, primarily regulate leaf senescence in response to blue light signals. Our results show that GmCRY1s interact with DELLA proteins under light-activated conditions, stabilizing them and consequently suppressing the transcription of GmWRKY100 to delay senescence. Conversely, LBL reduces the interaction between GmCRY1s and the DELLA proteins, leading to their degradation and premature senescence of leaves. Our findings suggest a GmCRY1s-GmDELLAs-GmWRKY100 regulatory cascade that is involved in mediating LBL-induced leaf senescence in soybean, providing insight into the mechanism of how light signals regulate leaf senescence. Additionally, we generate GmWRKY100 knockout soybeans that show delayed leaf senescence and improved yield under natural field conditions, indicating potential applications in enhancing soybean production by manipulating the leaf senescence trait.

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Chlorophyll retention caused by STAY-GREEN (SGR) gene mutation enhances photosynthetic efficiency and yield in soybean hybrid Z1

Cited by 5 publications

References 41 publications

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars

The mechanism of low blue light-induced leaf senescence mediated by GmCRY1s in soybean

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