Chlorophyll-protein and polypeptide composition of Mn-deficient sugar beet thylakoids

Abadı́a, Javier; Nishio, John N.; Terry, Norman

doi:10.1007/bf00014677

Cited by 14 publications

(7 citation statements)

References 33 publications

(58 reference statements)

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Section: Introductionmentioning

confidence: 99%

“…The data obtained were compared with those of a standard Fl hybrid of the same species. The mutant was depleted in chlorophyll b relative to the hybrid and it had a higher photosystem II/photosystem I reaction center (Q/P7") (1,12,17,26).Investigation of the effects of mutations of genes coding for specific components of the thylakoid membrane permit greater precision in the interpretation of the structural/functional role of that component than would be possible for many environmental perturbations of thylakoid structure. In our laboratory (N. T.) we are studying currently a low Chl sugar beet mutant which exhibits high rates ofphotosynthesis and electron transport per unit Chl similar to those described by Miles (23): the mutation apparently affects some specific polypeptides of the LHCP.3 Here, we characterize the structural organization of thylakoid membranes of the low Chl mutant by combining spectroscopic analysis with gel electrophoresis.…”

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confidence: 99%

“…In turn, such measurements enabled the estimation of the Chl antenna size of each PS in situ ( 18). Other (1,12,17,26).…”

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confidence: 99%

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Photochemical Apparatus Organization in the Chloroplasts of Two Beta vulgaris Genotypes

Abadı́a¹,

Glick²,

Taylor³

et al. 1985

Plant Physiol.

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The composition and structural orpnization of thylakoid membranes of a low chlorophyll mutant of Beta rulgaris was investigated using spectroscopic, kinetic and electrophoretic techniques. The data obtained were compared with those of a standard Fl hybrid of the same species. The mutant was depleted in chlorophyll b relative to the hybrid and it had a higher photosystem II/photosystem I reaction center (Q/P7") (1,12,17,26).Investigation of the effects of mutations of genes coding for specific components of the thylakoid membrane permit greater precision in the interpretation of the structural/functional role of that component than would be possible for many environmental perturbations of thylakoid structure. In our laboratory (N. T.) we are studying currently a low Chl sugar beet mutant which exhibits high rates ofphotosynthesis and electron transport per unit Chl similar to those described by Miles (23): the mutation apparently affects some specific polypeptides of the LHCP.3 Here, we characterize the structural organization of thylakoid membranes of the low Chl mutant by combining spectroscopic analysis with gel electrophoresis. The mutant genotype is compared with the standard sugar beet genotype (an Fl hybrid) used in this laboratory, for which a large amount of biochemical and physiological data is available. Spectroscopic estimates of the amounts of Chl associated with PSII and PSI, and of the stoichiometries of the reaction centers of the two photosystems were compared with those obtained using three different gel electrophoresis systems. MATERIALS AND METHODS

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Photochemical Apparatus Organization in the Chloroplasts of Two Beta vulgaris Genotypes

Abadı́a¹,

Glick²,

Taylor³

et al. 1985

Plant Physiol.

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“…For gel electrophoresis, thylakoids were extracted as previously described (12) and stored in 50 mm Tricine, pH 8.0 (1). For electron transport, thylakoids were extracted by the method of Ball et al (4) except that chloroplasts were not osmotically shocked.…”

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confidence: 99%

Chlorophyll-Proteins and Electron Transport during Iron Nutrition-Mediated Chloroplast Development

Nishio

Abadı́a²,

Terry³

1985

Plant Physiol.

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Chlorophyll-protein complexes and electron transport activities were measured during iron nutrition-mediated chloroplast development in sugar beet (Beta vwlgaris L. cv F58-554H1). Results showed that the chlorophyll-protein complexes associated with the reaction centers of photosystem I (CP1) and photosystem II (CPa) and the electron transport activities of these two photosystems per leaf area increased rapidly during the first 24 to 48 hours of iron resupply to iron-deficient sugar beet plants. Bulk chlorophyll and the amounts of light-harvesting chlorophyllproteins increased after a lag period of 24 hours. The changes in chlorophyll-proteins with time were apparently the cause ofan initial increase, then decrease, in the chlorophyll a/b ratio during iron resupply. There was evidence that iron deficiency diminished photosystem I more than photosystem II. We propose that there are two distinct phases in iron nutrition-mediated chloroplast development: (a) the commencement of the synthesis of the lipid matrix of the thylakoid membrane, including a fully functioning electron transport (and photosynthetic) system, during the first 24 hours of iron resupply, and (b) after 24 to 48 hours, the formation of the bulk of the thylakoid proteins, including the lightharvesting chlorophyll-proteins with which the large increase in total chlorophyll is associated.Earlier work showed that when Fe was resupplied to Fedeficient sugar beet plants, the synthesis of thylakoid membrane lipids (galactolipids) was linear over a 96-h period (12). The PSI electron transport components, P70o and Cyt f, also increased linearly with time (13). However, total Chl and total thylakoid protein exhibited a lag of24 h before maximum rates ofsynthesis were attained (12). Chl a/b ratios have been shown to increase over the first 24 h of resupply, then decrease (12,13,17).These experimental observations suggest that during Fe nutrition-mediated chloroplast development, the membrane matrix is laid down first with reaction centers and electron carriers, and that after 24 h there is increased synthesis of the LHCPs3 (majority of Chl) and other thylakoid proteins. Thus, during the first 24 h, the reaction center Chl-proteins (mainly Chl a-containing) would be accumulated more rapidly than the Chl b (and Chl a) containing LHCPs. Thereafter, the LHCPs would (according to

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“…Sultana et al (2001) also observed that foliar spray of Mn partially minimize the salt induced nutrient deficiency and photosynthesis. Abadia et al (1986) concluded that examining the chlorophyll-protein and peptide composition of manganese deficient and control sugar beet thylakoids and found decrease in manganese decreased several thylakoid polypeptides, including chlorophyll b containing 30 kDa chlorophyll-protein complex. Supplementing Mn improved the chlorophyll contents; foliar Mn application rescued more chlorophyll contents as compared to root application.…”

Section: Discussionmentioning

confidence: 99%

Effect of Manganese Application on PS-II Activity in Rice under Saline Conditions

Tabassam¹,

Kanwal²,

Naqvi³

et al. 2016

IJAB

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Salinity reduces the uptake of manganese (Mn) and induces its deficiency, which adversely affects photosynthesis-related photosystem-II activity, hence growth is hampered. The objective of the study was to investigate the effects of Mn application on the photosystem-II activity on the isolated thylakoid membranes of the rice. A hydroponic study was carried out in the glasshouse of National Agriculture Research Centre, Islamabad, Pakistan on two rice varieties, Pakhal and KS-282, using three replicates. Three salt concentrations, namely 0, 25, 50 mM NaCl were used. Manganese sulphate was used for foliar and root application with four concentrations (0, 2, 4, 8 µg Mn mL -1 ). Salinity decreased the concentration of Mn, while the application of Mn, increased the contents of Mn in shoot and root. Increased salt concentration caused a decrease in the chlorophyll contents while Mn application increased chlorophyll contents across all the salinity levels, with maximum increase at 2 µg Mn mL -1 . Photosystem-II activity was inhibited by increasing salt concentration, while Mn treatment rescued this inhibitory affect, with maximum photosystem-II activity recovery at 2 µg Mn mL -1 level in both varieties. Quite high concentration of Mn was observed in root application methods as compared to foliar but it failed to recover salinity impact on photosystem-II and photosynthesis. Applying Mn as foliar increased tolerance to salinity at 50 mM NaCl level while root application was found better at 25 mM NaCl concentration in culture solution.

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Chlorophyll-protein and polypeptide composition of Mn-deficient sugar beet thylakoids

Cited by 14 publications

References 33 publications

Photochemical Apparatus Organization in the Chloroplasts of Two Beta vulgaris Genotypes

Photochemical Apparatus Organization in the Chloroplasts of Two Beta vulgaris Genotypes

Chlorophyll-Proteins and Electron Transport during Iron Nutrition-Mediated Chloroplast Development

Effect of Manganese Application on PS-II Activity in Rice under Saline Conditions

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