Chlorophyll Organization and Function in Green Photosynthetic Bacteria

Olson, John M.

doi:10.1562/0031-8655(1998)067<0061:coafig>2.3.co;2

Cited by 87 publications

(153 citation statements)

References 69 publications

(103 reference statements)

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“…Absorption of the α-band (770 nm) of peak 6 and its fl uorescence (782 nm) intensity (data not shown) suggested that the amount of bacteriochlorophyll a, another photosynthetic pigment of some purple sulfur bacteria, was very low. Bacteriochlorophyll a may not be derived from purple sulfur bacteria, but from green sulfur bacteria, because the photosystem of green sulfur bacteria also contains bacteriochlorophyll a (Olson 1998). Our results indicate that picophytoplankton coexist with green sulfur bacteria in the halocline of Lake Suigetsu.…”

Section: Discussionmentioning

confidence: 70%

Abundance of picophytoplankton in the halocline of a meromictic lake, Lake Suigetsu, Japan

et al. 2007

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Numerous (0.5 to 4.8 × 10 5 cells/ml), small phytoplankton (smaller than 0.5-1 × 1-2 µm in cell size, picophytoplankton) were distributed in the halocline (depth 2-12 m, 4-14 practical salinity units) of the saline meromictic lake, Lake Suigetsu (35°35′ N, 135°52′ E), located in the central part of the coast of Wakasa Bay along the Japan Sea in Fukui Prefecture, Japan. Vertical distribution of phytoplankton revealed that the maximum number of picophytoplankton was always observed near or a little deeper than the oxic-anoxic boundary layer (depth 5-6 m); they were dominant phytoplankton in the water layer deeper than the oxic-anoxic boundary from July to late September 2005. Spectral analysis of autofl uorescence emitted from the particle fractions smaller than 5 µm measured with a spectrofl uorometer and from individual cells measured with a microscope photodiode array detector revealed that the major component of picophytoplankton was phycoerythrinrich, unicellular cyanobacteria (picocyanobacteria). Eukaryotic phytoplankton about 2.5 µm in diameter were also found, but the numbers were low. Fluorescence intensity of chlorophyll a at 685 nm (room temperature) emitted from the particle fractions smaller than 5 µm was increased by the addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. These observations indicated that at least some picophytoplankton had a functional photosystem II in the halocline where sulfi de, the potential inhibitor of oxygenic photosynthesis, was always present. The large abundance together with their physiological potency suggest that picophytoplankton are one of the important primary producers in the halocline of Lake Suigetsu.Electronic supplementary material Supplementary material is available in the online version of this article at http://dx.

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Section: Discussionmentioning

confidence: 70%

Abundance of picophytoplankton in the halocline of a meromictic lake, Lake Suigetsu, Japan

et al. 2007

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“…The bulk pigment of the chlorosome is bacterial chlorophyll c, and can be found in 10-30 long aggregated rods, without intervening protein. This large array efficiently transfers the absorbed energy to an intermediate bacterial chlorophyll a/protein complex called the base-plate, which transmits the energy into the reaction center (Blankenship et al 1995;Olson 1998;Montano et al 2003). No homology exists between the chlorosome and PBS on either the level of pigment aggregation or protein sequence, and thus it can be assumed that these two complexes evolved separately, to utilize the available volume outside the thylakoid membrane for light-harvesting.…”

Section: Energy Transfer Within the Pbsmentioning

confidence: 99%

Elucidation of the molecular structures of components of the phycobilisome: reconstructing a giant

2005

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The molecular architectures of photosynthetic complexes are rapidly becoming available through the power of X-ray crystallography. These complexes are comprised of antenna complexes, which absorb and transfer energy into photochemical reaction centers. Most reaction centers, found in both oxygenic and non-oxygenic species, are connected to transmembrane chlorophyll containing antennas, and the crystal structures of these antennas contain information on the structure of the entire complex as well as clear indications on their modes of functional association. In cyanobacteria and red alga, most of the Photosystem II associated light harvesting is performed by an enormous (3-7 MDa) membrane attached complex called the phycobilisome (PBS). While the crystal structures of many isolated components of different PBSs have been determined, the structure of the entire complex as well as its manner of association with Photosystem II can only be suggested. In this review, the structural information obtained on the isolated components will be described. The structural information obtained from the components provides the basis for the modeled reconstruction of this giant complex.

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“…Photosynthetic pigment-protein complexes which have evolved in bacteria, algae, and plants for light harvesting, energy transfer, and charge separation, are examples of fluorophores (Olson 1998;Blankenship et al 1995;Kargul et al 2012). Typically, they consist of pigments-(bacterio) chlorophylls and carotenoids-embedded in a protein scaffold.…”

Section: Introductionmentioning

confidence: 99%

Silver island film substrates for ultrasensitive fluorescence detection of (bio)molecules

et al. 2015

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A silver island film (SIF) substrate was used to demonstrate that Metal-Enhanced Fluorescence (MEF) is a powerful tool to enable detection of emission from (bio)molecules at very low concentrations. The experiments were carried out with the Fenna-Matthews-Olson (FMO) pigment-protein complex from the photosynthetic green sulfur bacterium Chlorobaculum tepidum. FMO was diluted to a level, at which no emission was detectable on a glass substrate. In contrast, the fluorescence of FMO was readily observed on the SIF substrate, even though the emission wavelength of FMO is displaced by over 300 nm from the maximum of the plasmon resonance of the SIF layer. Estimated enhancements of the fluorescence intensity of FMO on SIF are about 40-fold. The enhancement factor correlates with the improvement of the signal-to-noise ratio for FMO emission on SIF substrates.

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Chlorophyll Organization and Function in Green Photosynthetic Bacteria

Cited by 87 publications

References 69 publications

Abundance of picophytoplankton in the halocline of a meromictic lake, Lake Suigetsu, Japan

Abundance of picophytoplankton in the halocline of a meromictic lake, Lake Suigetsu, Japan

Elucidation of the molecular structures of components of the phycobilisome: reconstructing a giant

Silver island film substrates for ultrasensitive fluorescence detection of (bio)molecules

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