Chlorophyll fluorescence for non-destructive measurement of flavonoids in broccoli

Bengtsson, Gunnar; Schöner, R.; Lombardo, Emanuele; Schöner, Jennifer; Borge, Grethe Iren A.; Bilger, Wolfgang

doi:10.1016/j.postharvbio.2005.11.003

Cited by 26 publications

(15 citation statements)

References 17 publications

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“…In contrast to traditional remote sensing techniques, ChlF produced solely by plants, rapidly captures the specific and instantaneous change of plants' physiology status and response to various nutritional treatments, heat, and water conditions [4,5]. Among all the ChlF parameters, Fv/Fm, which is the ratio of variable to maximal fluorescence, is the initial maximal efficiency of photons captured by open photosystem II (PSII) reaction centers [6], and is a widely used parameter representing the health and growth of plants [7][8][9]. The Fv'/Fm' parameter, which is the efficiency of energy harvesting by oxidized (open) PSII reaction centers in light, serves as an indicator to assess plant stress, nutrient, and health status [10].…”

Section: Introductionmentioning

confidence: 99%

Quantifying Chlorophyll Fluorescence Parameters from Hyperspectral Reflectance at the Leaf Scale under Various Nitrogen Treatment Regimes in Winter Wheat

Jiang

Colombo

et al. 2019

Remote Sensing

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Chlorophyll fluorescence (ChlF) parameters, especially the quantum efficiency of photosystem II (PSII) in dark- and light-adapted conditions (Fv/Fm and Fv’/Fm’), have been used extensively to indicate photosynthetic activity, physiological function, as well as healthy and early stress conditions. Previous studies have demonstrated the potential of applying hyperspectral data for the detection of ChlF parameters in vegetation. However, the performance of spectral features that have been documented to estimate ChlF is not ideal and is poorly understood. In this study, ChlF parameters and leaf reflectance were collected in two field experiments involving various wheat cultivars, nitrogen (N) applications, and plant densities, during the growing seasons of 2014 to 2015 and 2015 to 2016. Three types of spectral features, including vegetation indices (VIs), red edge position (REP), and wavelet features, were used to quantify ChlF parameters Fv/Fm and Fv’/Fm’. The results indicated that traditional chlorophyll fluorescence vegetation indices (ChlF VIs), such as the curvature index (CUR) and D705/D722 were capable of detecting Fv/Fm and Fv’/Fm’ under various scenarios. However, the wavelet-based REP (WREP-S4) and the wavelet feature (WF) (704 nm, scale 4) yielded higher accuracy than other spectral features in calibration and validation datasets. Moreover, the bands used to calculate WREP-S4 and WF (704 nm, scale 4) were all centered in the red edge region (680 to 760 nm), which highlighted the role of the red edge region in tracking the change of active ChlF signal. Our results are supported by previous studies, which have shown that the red edge region is vital for estimating the chlorophyll content, and also the ChlF parameters. These findings could help to improve our understanding of the relationships among active ChlF signal and reflectance spectra.

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Section: Introductionmentioning

confidence: 99%

Quantifying Chlorophyll Fluorescence Parameters from Hyperspectral Reflectance at the Leaf Scale under Various Nitrogen Treatment Regimes in Winter Wheat

Jiang

Colombo

et al. 2019

Remote Sensing

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“…Epidemiological studies suggest that high dietary intake of fruit and vegetables protects against tumorigenesis in many tissues (Liu et al 2000;Knekt et al 2002). The health beneficial effects seem to be connected to their high content of vitamins, minerals, dietary fibre and other constituents such as floavonoids, carotenoids and glucosinolates (Bengtsson et al 2006;Monero et al 2010). It is well known that certain quality attributes like nutrients content, texture, colour and flavour are affected during storage of untreated vegetables (Viña et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Influence of different blanching methods on colour, ascorbic acid and phenolics content of broccoli

et al. 2015

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Lack of nutrients in cooking water, high energetic costs, high water consumption and recycling are some drawbacks of vegetable blanching. Those disadvantages could be bypassed using microwave blanching. Three blanching methods (microwave, boiling water and steaming) were compared in this study in order to determine their effects on some functional properties of broccoli. In addition, the thermal damage on broccoli colour was evaluated. The effectiveness of each blanching process was performed measuring the lost of peroxidase activity, that results more rapidly in microwaves and steam treatments (50 and 60 s respectively) than in boiling water treatment (120 s). The colour indexes did not allow to discriminate a significant difference among treatments. The increase of treatment time caused a vitamin C decrease in samples blanched by boiling water and steam; this trend was not observed in microwaved samples. The phenols content did not significantly vary depending both on type and on time of treatment.

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“…As shown in Agati et al (2005), Barthod et al (2007), Bengtsson et al (2006), Cerovic et al (2002), Hagen et al (2006), and Merzlyak et al (2008b), the application of chlorophyll fluorescence for nondestructive analysis of plant constituents able to compete with chlorophyll in light absorption is a promising tool. In anthocyanin-free fruit, the assessment of flavonols turned out to be feasible using the CFE ratio at 440 nm.…”

Section: Approaches To Estimation Of the Photoprotective Pigment Effimentioning

confidence: 99%

“…(Reprinted from Solovchenko et al (2010a, b) with permission from Elsevier) unsuccessful, maybe due to the involvement of different carotenoid pools in light harvesting and in screening (Merzlyak and Solovchenko 2002;Merzlyak et al 2008b;Solovchenko et al 2006). It appears that at present spectral reflectance with simpler quantitative measurements of (re)emitted light and simple relations to the content of a pigment of interest represents a more reliable and flexible technique for nondestructive pigment assessments, although the specific advantages of the fluorescence analysis pointed out in the literature (Agati et al 2005;Barthod et al 2007;Bengtsson et al 2006;Cerovic et al 2002;Hagen et al 2006;Merzlyak et al 2008b) are of considerable importance.…”

Section: Approaches To Estimation Of the Photoprotective Pigment Effimentioning

confidence: 99%

Quantification of Screening Pigments and Their Efficiency In Situ

Solovchenko

2010

Springer Series in Biophysics

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This chapter deals with nondestructive quantification of screening pigment content and estimation of the efficiency of screening pigments. The first part of the chapter describes the approaches for the employment of the relationships between changes in screening pigment content and composition and the effects screening pigments exert on reflection of light by plants (considered in detail in the previous chapter) for quantification of screening pigments in situ. The second part considers the current approaches for estimating the efficiency of screening by different pigments in planta.The investigation of the physiological significance of screening pigments in plants requires information about pigment content and the photoprotective capability of the pigments. The traditional biochemical procedures are often not suitable for the solution of this problem since they do not provide information on the in vivo screening efficiency and do not take into account the issues related to in planta spectroscopy of pigments (see Sect. 5.1). During recent decades, a number of alternative approaches for nondestructive quantification of screening pigments and estimation of their efficiency in situ have evolved. Roughly, they can be divided into two major groups (1) reflectance-based, i.e., employing optical reflectance spectroscopy, and (2) fluorescence-based, i.e., using chlorophyll as an internal fluorescent probe. Both techniques are briefly considered below with an emphasis on the advances made in the laboratories of the author and his colleagues. Optical Reflectance-Based Techniques for Nondestructive Screening Pigment AssessmentSpectrophotometry of tissue extracts is a common method for the analysis of plant pigments in physiological and biochemical studies. The application of this method inevitably involves destruction of the sample; it is time-consuming and is prone to

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Chlorophyll fluorescence for non-destructive measurement of flavonoids in broccoli

Cited by 26 publications

References 17 publications

Quantifying Chlorophyll Fluorescence Parameters from Hyperspectral Reflectance at the Leaf Scale under Various Nitrogen Treatment Regimes in Winter Wheat

Quantifying Chlorophyll Fluorescence Parameters from Hyperspectral Reflectance at the Leaf Scale under Various Nitrogen Treatment Regimes in Winter Wheat

Influence of different blanching methods on colour, ascorbic acid and phenolics content of broccoli

Quantification of Screening Pigments and Their Efficiency In Situ

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