Chlorophyll fluorescence—a practical guide

Maxwell, Kate; Johnson, Giles N.

doi:10.1093/jxb/51.345.659

Cited by 2,504 publications

(2,450 citation statements)

References 31 publications

Supporting

Mentioning

2,258

Contrasting

Unclassified

105

Order By: Relevance

“…The maximum photochemical efficiency of PSII (Fv/Fm) was calculated as (Fm − F 0 )/Fm. The quantum yield of PSII (Y(II)) and nonphotochemical quenching (NPQ) were calculated as follows: Y(II) = (Fm′ − F 0 )/Fm′, NPQ = (Fm − Fm′)/Fm′ (Maxwell and Johnson, 2000). …”

Section: Methodsmentioning

confidence: 99%

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Zhang

Wang

et al. 2016

Biotech & Bioengineering

View full text Add to dashboard Cite

The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high‐value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark‐grown cells. Although moderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5 mg L−1 day−1) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark‐grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high‐light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5 mg L−1 day−1 by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark‐grown cells under photo‐induction conditions. Biotechnol. Bioeng. 2016;113: 2088–2099. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

show abstract

Section: Methodsmentioning

confidence: 99%

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Zhang

Wang

et al. 2016

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…The red edge is also closely related to chlorophyll fluorescence (Zarco-Tejada et al, 2003), a powerful and widely used technique to detect plant stress (Maxwell and Johnson, 2000). Therefore, the red edge may be a general indicator of plant status by integrating multiple plant responses including biochemistry, physiology, growth and mortality .…”

Section: Mechanisms and Potential For Monitoring Plant Stress Using Tmentioning

confidence: 99%

Monitoring plant response to phenanthrene using the red edge of canopy hyperspectral reflectance

Zhu

Chen

Wang

et al. 2014

Marine Pollution Bulletin

View full text Add to dashboard Cite

a b s t r a c tTo investigate the mechanisms and potential for the remote sensing of phenanthrene-induced vegetation stress, we measured field canopy spectra, and associated plant and soil parameters in the field controlled experiment in the Yellow River Delta of China. Two widely distributed plant communities, separately dominated by reed (Phragmites australis) and glaucous seepweed (Suaeda salsa), were treated with different doses of phenanthrene. The canopy spectral changes of plant community resulted from the decreases of biomass and foliar projective coverage, while leaf photosynthetic pigment concentrations showed no significance difference among treatments. The spectral response to phenanthrene included a flattened red edge, with decreased first derivative of reflectance. The red edge slope and area consistently responded to phenanthrene, showing a strong relationship with aboveground biomass, coverage and canopy pigments density. These results suggest the potential of remote sensing and the importance of field validation to correctly interpret the causes of the spectral changes.

show abstract

“…Using a portable photosynthesis system LI-COR 6400 (Li-Cor, Lincoln, NE), maximum quantum yield of PSII (F v /F m ) and minimal chl a fluorescence following dark incubation (F 0 ) were determined. Quantum efficiency of electron transport through PSII (ΦPSII) was measured after adaptation to day light for 20 min (Maxwell and Johnson, 2000). Rates of CO 2 assimilation were determined at a flux density of 500 µmol m -2 s -1 and a CO 2 concentration of 380 µmol mol -1 .…”

Section: Production Of Anti-atsgr Antiserum and Immunoblot Analysismentioning

confidence: 99%

Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway

2008

View full text Add to dashboard Cite

Chlorophyll fluorescence—a practical guide

Cited by 2,504 publications

References 31 publications

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Monitoring plant response to phenanthrene using the red edge of canopy hyperspectral reflectance

Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway

Contact Info

Product

Resources

About