Chlorophyll Fluorescence: A Practical Approach to Study Ecophysiology of Green Plants

Mishra, Amarendra Narayan

doi:10.1007/978-3-319-93233-0_5

Cited by 12 publications

(6 citation statements)

References 108 publications

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“…The Y(NPQ) represents heat dissipation as a plant mechanism of photo-protection, state transition, and photo-inhibition [ 56 , 57 ]. It has been described that Y(NPQ) increases under stress conditions such as high light intensity or photoinhibition, salinity, heavy metal toxicity, drought, or chilling [ 58 ]. Therefore, TgERF1 transgenic plants maintained lower amounts of Y(NPQ) compared to WT plants, which could probably mean that the Y(NPQ) gene helps regulate the heat dissipation and photo-protective mechanisms, through the improved absorption and transmission of light energy.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of TgERF1, a Transcription Factor from Tectona grandis, Increases Tolerance to Drought and Salt Stress in Tobacco

Oliveira

Hurtado

Martínez‐Andújar

et al. 2023

IJMS

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Teak (Tectona grandis) is one of the most important wood sources, and it is cultivated in tropical regions with a significant market around the world. Abiotic stresses are an increasingly common and worrying environmental phenomenon because it causes production losses in both agriculture and forestry. Plants adapt to these stress conditions by activation or repression of specific genes, and they synthesize numerous stress proteins to maintain their cellular function. For example, APETALA2/ethylene response factor (AP2/ERF) was found to be involved in stress signal transduction. A search in the teak transcriptome database identified an AP2/ERF gene named TgERF1 with a key AP2/ERF domain. We then verified that the TgERF1 expression is rapidly induced by Polyethylene Glycol (PEG), NaCl, and exogenous phytohormone treatments, suggesting a potential role in drought and salt stress tolerance in teak. The full-length coding sequence of TgERF1 gene was isolated from teak young stems, characterized, cloned, and constitutively overexpressed in tobacco plants. In transgenic tobacco plants, the overexpressed TgERF1 protein was localized exclusively in the cell nucleus, as expected for a transcription factor. Furthermore, functional characterization of TgERF1 provided evidence that TgERF1 is a promising candidate gene to be used as selective marker on plant breeding intending to improve plant stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Overexpression of TgERF1, a Transcription Factor from Tectona grandis, Increases Tolerance to Drought and Salt Stress in Tobacco

Oliveira

Hurtado

Martínez‐Andújar

et al. 2023

IJMS

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“…7 ). It is known that the electron transport rate increases proportionally with carbon fixation and that stress conditions can drastically decrease these two parameters ( Mishra, 2018 ). Therefore, the observed low photosynthesis values and electron transport rate are indeed expected in WT plants under salt stress but not in TgNAC01 transgenic lines ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…NPQ represents a heat dissipation, which is a plant mechanism of photo-protection, state transition and photo-inhibition. NPQ increases under stress conditions, such as high light intensity or photoinhibition, salinity, heavy metal toxicity, drought or chilling ( Mishra, 2018 ). Transgenic plants maintained relatively the same amount of NPQ in the different salt concentrations compared to WT plants ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of theTectona grandis TgNAC01regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants

Hurtado

Oliveira

Gómez-Espinoza

et al. 2022

PeerJ

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NAC transcription factors play critical roles in xylem secondary development and in regulation of stress response in plants. NAC proteins related to secondary cell wall development were recently identified and characterized in Tectona grandis (teak), one of the hardwood trees of highest economic importance in the world. In this work, we characterized the novel TgNAC01 gene, which is involved in signaling pathways that mediate teak response to stress. Abscisic acid (ABA) increases TgNAC01 expression in teak plants. Therefore, this gene may have a role in signaling events that mediate ABA-dependent osmotic stress responsive in this plant species. Stable expression in tobacco plants showed that the TgNAC01 protein is localized in the cell nucleus. Overexpression of TgNAC01 in two out three independent transgenic tobacco lines resulted in increased growth, leaf senescence and salt tolerance compared to wild type (WT) plants. Moreover, the stress tolerance of transgenic plants was affected by levels of TgNAC01 gene expression. Water potential, gas exchange and chlorophyll fluorescence were used to determine salt stress tolerance. The 35S:TgNAC01-6 line under 300 mM NaCl stress responded with a significant increase in photosynthesis rate, stomatal conductance, transpiration and carboxylation efficiency, but lower water potential compared to WT plants. The data indicate that the TgNAC01 transcription factor acts as a transcriptional activator of the ABA-mediated regulation and induces leaf senescence.

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“…The restrictions to carbon assimilation enforced by stomatal termination can result in an imbalance between the electron requirement for photosynthetic movements and photochemical activities at photosystem II, which may lead to photoinhibitory destruction of photosystem II reaction centers. The main defense mechanism involved in photo-inhibitory effects in plant cells is probably the increase in non-photochemical quenching energy dissipation [14]. Lee et al [15] reported that maximum photochemical efficiency of photosystem II (F v /F m , where F v is the variable fluorescence and F m is the maximum fluorescence) is still high under tolerable salt toxicity, while plant biomass decreases under this salinity level.…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll a fluorescence of safflower affected by salt stress and hormonal treatments

Ghassemi‐Golezani

Hosseinzadeh-Mahootchi

Farhangi‐Abriz

2020

SN Appl. Sci.

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Salicylic and jasmonic acids are classified as stress hormones, which can affect numerous physiological characteristics of plants such as electron transporting systems in thylakoid membrane under normal and stressful conditions. A greenhouse experiment was conducted with factorial arrangement based on a randomized complete block design with three replications to assess the possible effects of salicylic acid (1 mM) and jasmonic acid (0.5 mM) on different parameters of chlorophyll a fluorescence in safflower (Carthamus tinctorius L. cv. Goldasht) under salt stress [0, 4, 8, and 12 dS m −1 NaCl (about 0, 40, 80 and 120 mM NaCl, respectively)]. Leaf chlorophyll content index, relative water content, leaf area and seed yield were decreased with increasing salinity. Salinity caused a significant decrease in the variable fluorescence, the maximum quantum yield of photosystem II, efficiency of water-splitting complex on the donor side of the photosystem II and total performance index (PI total ). The highest time needed to reach maximum fluorescence was recorded at 12 dS m −1 . Maximum fluorescence and PI total were increased, but initial fluorescence and maximum fluorescence were significantly decreased by salicylic acid and jasmonic acid treatments under saline conditions. Salicylic acid and jasmonic acid considerably improved the variable fluorescence, maximum quantum yield of photosystem II, the efficiency of water-splitting complex, PI total , physiological parameters and seed yield under salinity. In this way, foliar application of salicylic acid and jasmonic acid could play a key role in salt stress tolerance of safflower plants.

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Chlorophyll Fluorescence: A Practical Approach to Study Ecophysiology of Green Plants

Cited by 12 publications

References 108 publications

Overexpression of TgERF1, a Transcription Factor from Tectona grandis, Increases Tolerance to Drought and Salt Stress in Tobacco

Overexpression of TgERF1, a Transcription Factor from Tectona grandis, Increases Tolerance to Drought and Salt Stress in Tobacco

Overexpression of theTectona grandis TgNAC01regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants

Chlorophyll a fluorescence of safflower affected by salt stress and hormonal treatments

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