Chlorophyll and Carotenoid Destruction in the Absence of Light in Seedlings of Zea Mays L

Frank, Sylvia; Kenney, Arthur L.

doi:10.1104/pp.30.5.413

Cited by 30 publications

(7 citation statements)

References 6 publications

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“…Most publications dealing with the analyses and interrelationships of the plastid pigments of maize and Avena have been confined to groups of pigments rather than specific plastid pigments (3,4,5,13). Since the changes in a group of pigments do not necessarily reflect the behavior of the individual pigments, knowledge about the specific pigment changes is desirable.…”

mentioning

confidence: 99%

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Plastid Pigment Changes in the Early Seedling Leaves of Zea Mays L

Kay

Phinney

1956

Plant Physiol.

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confidence: 99%

“…Leaf samples for analyses were obtained in the following manner: the second seedling leaf was removed from 15 different plants and the 15 leaves divided into 3 samples of 5 leaves each. Using a steel die, a section 7 by 52 mm was punched from the distal end of each leaf.…”

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confidence: 99%

Plastid Pigment Changes in the Early Seedling Leaves of Zea Mays L

Kay

Phinney

1956

Plant Physiol.

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“…Such activity, in association with the changes recorded in rate of petiole elongation (see Fig. This resulted in decreases in the normal life expectancy of leaves, because of chlorophyll degradation (Franck & Kenney 1955) followed by accelerated leaf senescence under the intense shading that occurred. Under these conditions, earlier formed leaves were continually overtopped by those formed later.…”

Section: Discussionmentioning

confidence: 90%

The Leaf Growth of Trifolium Repens as Influenced by Seasonal Changes in the Light Environment

Brougham¹

1962

The Journal of Ecology

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. British Ecological Society is collaborating with JSTOR to digitize, preserve and extend access to Journal of Ecology.

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“…Frank (8,9) and others have suggested that the carotenoids and the phytyl portion of the chlorophyll molecule probably arise from colorless precursors common to both. On the basis of the unitary action of the gene (3)(4)(5), genetical evidence also indicates that the carotenoids and chlorophylls are closely interrelated in their biosynthesis, since many single genes are known to affect the presence of the carotenoids as well as the chlorophylls.…”

Section: Discussionmentioning

confidence: 99%

The Control of Plastid Pigment Formation by a Virescent Gene, Pale-Yellow-1, of maize.

Kay

Phinney

1956

Plant Physiol.

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The effects of certain environmental factors upon the phenotypic expression of a single-gene mutation, pale-yellow-1, of maize have been described (13). In brief, the observations showed that the mutant seedling is initially uniform pale-yellow in color when grown in either light or darkness. However, when exposed to light at 250 C the mutant seedlings become green in color approximately 8 days after planting. Plastid size and number, type of culture media used, length of previous exposure to light and the presence of the endosperm are not factors in this delayed appearance of pigments.This paper will present quantitative information on the pigment content and pigment changes which occur in the mutant, pale-yellow-1, of maize. In particular, an attempt will be made to establish the relationship of the mutant gene to the accumulation of specific carotenoid pigments and the chlorophylls.This will be done in order to determine the basis for the differences in color between the mutant and its normal sibling (13). In addition, it is hoped that some information about the biochemical relationship of the carotenoids to the chlorophylls will be obtained from these observations. METHODS The plants used in this study were germinated from seeds which were found to produce seedlings in a ratio of 3 normals to 1 mutant. All seedlings were grown in a constant temperature chamber at 25 -0.50 C. Light (700 ft-c) was supplied by 13 slimline fluorescent lamps labeled 45000 white. A description of the culture methods, light source and the constant temperature chamber has been reported (13 When the samples were analyzed for carotenoids, the pigments were extracted with methanol, the chlorophylls saponified and the carotenoids transferred to a mixture of ethyl ether and petroleum ether. The carotenols were chromatographically resolved into individual components on a magnesia column, while the carotenes passed through the column and were recovered as a binary mixture in the perculate. Samples analyzed for chlorophylls were extracted with methanol and the chlorophylls transferred to petroleum ether. The chlorophylls were separated from the carotenoids and resolved into chlorophyll a and chlorophyll b by chromatographing the petroleum ether solution on a starch column. Protochlorophyll a was extracted from the leaf samples and chromatographically isolated by the method of Koski and Smith (12). The amounts of chlorophyll a, chlorophyll b, protochlorophyll a, lutein, violaxanthin b, and flavoxanthin c were calculated from the absorbance, at their respective maxima, and the specific absorption coefficients given by Zscheile and Comar (17), Strain (14) and Koski and Smith (12). The amounts of beta carotene and neo-beta carotene were calculated from the absorbance of the binary solution by the method of Beadle and Zscheile (2).EXPERIMENTAL AND RESULTSPIGAIENT CONTENT OF DARK-GROWN MUTANT AND NORMAL SEEDLINGS: When grown at 250 C, 7-dayold dark-grown mutant seedlings are pale-yellow in color, while dark-grown normal seedlings are brightyell...

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Chlorophyll and Carotenoid Destruction in the Absence of Light in Seedlings of Zea Mays L

Cited by 30 publications

References 6 publications

Plastid Pigment Changes in the Early Seedling Leaves of Zea Mays L

Plastid Pigment Changes in the Early Seedling Leaves of Zea Mays L

The Leaf Growth of Trifolium Repens as Influenced by Seasonal Changes in the Light Environment

The Control of Plastid Pigment Formation by a Virescent Gene, Pale-Yellow-1, of maize.

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