Chlorophyll-a fluorescence evaluation of PEG-induced osmotic stress on PSII activity in <i>Arabidopsis</i> plants expressing <i>SIP1</i>

Gururani, Mayank Anand; Venkatesh, Jelli; Ghosh, Ritesh; Strasser, Reto J.; Ponpandian, Lakshmi Narayanan; Bae, Hyeun‐Jong

doi:10.1080/11263504.2017.1403392

Cited by 22 publications

(23 citation statements)

References 44 publications

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“…After 10 days of exposure to PEG-induced stress, D220-81, D200-82 and D200-85 plants exhibited 51.4%, 49.2% and 35.7% higher total chlorophyll content respectively compared to the WT plants. Similar increased levels of chlorophyll content in SIP transgenic Arabidopsis plants under PEG-induced stress has recently been reported (Gururani et al 2017). Accumulation of H 2 O 2 in WT and D200 plants as a consequence of stress imposed by high levels of PEG was determined.…”

Section: Phenotypic Changes and Validation Through Ros And Chlorophylsupporting

confidence: 80%

“…To assess the changes in the photosynthetic machinery of WT and D200 plants subjected to PEG-induced osmotic stress, we measured the photosynthetic efficiency of these plants, in terms of Fv/ Fm, which indicates the maximum quantum yield of PSII. Abiotic stresses such are known to decrease the quantum efficiency of PSII photochemistry (Briantais et al 1996;Gururani et al 2017). Several reports suggest that measuring F v /F m to examine the photosynthetic efficiency of different crop plants subjected to various stresses is an efficient tool in stress physiological studies (De Ronde et al 2004;Yusuf et al 2010;Mathur et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The optimal value of F v /F m varies between a range of 0.79 and 0.83 for most plant species, and lower values indicate that the plant is lacking an optimal health state. Similarly, performance index (PI) is calculated from the recorded data which is the sum of overall expressions that represent a kind of internal force of the sample to resist stress thereby indicating the vitality of the samples used (Gururani et al 2012;Choi et al 2017;Ghosh et al 2017;Gururani et al 2017).…”

Section: Quantitative Real Time Pcrmentioning

confidence: 99%

“…The damage to DNA, lipids, and proteins caused by ROS molecules is known to be the result of oxidative stress (Foyer & Shigeoka 2011). The inimical effects of ROS accumulation in chloroplasts include the damage to photosystem II (PSII) D1 protein Gururani et al 2017;Sasi et al 2018), suppression of ROS-responsive chloroplastic enzymes (Kato & Sakamoto 2009), and disintegration of thylakoids (Gratão et al 2009;. To ensure survival under the adverse environmental conditions, plants have developed efficient antioxidant machinery that primarily has two arms, (i) enzymatic components like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) and; (ii) non-enzymatic antioxidants like ascorbic acid (AA), reduced glutathione (GSH), α-tocopherol, carotenoids, flavonoids, and the osmolyteproline (Gill & Tuteja 2010).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of osmotic stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene

Akilan

Halima

Sasi

et al. 2018

Journal of Plant Interactions

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Developing efficient stress-tolerant crops through genetic engineering remains one of the major challenges for plant biologists. Approximately 20-40% genes of the known eukaryotic genomes, encode proteins of unknown function which lack currently defined motifs or domains. In a previous study, large-scale yeast functional screening approach in potato was used and 69 genes were reported to have enhanced hyperosmotic stress tolerance of yeast. Twelve out of 69 genes were found to have stress tolerance against multiple stresses. One of those 12 identified genes (StD200) encodes a protein of unknown function. In this study, we evaluated the tolerance against PEGinduced osmotic stress in transgenic Arabidopsis plants expressing putative abiotic stressassociated D200 gene. The D200 plants exhibited higher accumulation of chlorophyll and proline and reduced levels of oxidants compared to the wild-type (WT) control plants when subjected to PEG-induced osmotic stress conditions. Our quantitative Real-Time PCR results also suggested an increased accumulation of mRNA transcripts of genes encoding three major antioxidant enzymes in PEG-treated D200 plants compared to WT. Furthermore, improved photosynthetic parameters, F v / F m and performance index in PEG-treated D200 plants indicated that potato D200 gene is a potential candidate gene for developing stress-tolerant crops in future.

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Section: Phenotypic Changes and Validation Through Ros And Chlorophylsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Quantitative Real Time Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of osmotic stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene

Akilan

Halima

Sasi

et al. 2018

Journal of Plant Interactions

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“…The parameters ABS/CS0, TR0/CS0, ET0/CS0 and DI0/CS0 were analyzed via the energy pipeline leaf model of phenomenological fluxes using Biolyzer 4HP software ( Figure 4E-H). This model typically provides information on the flow of energy from the chlorophyll antenna molecules to the photosynthetic electron transport chain via the RCs of PSII [29,30]. The capability of the plants in each group for light absorption (ABS/CS0), trapping, electron transport (TR0/CS0), and dissipation per cross-section of PSII (DI0/CS0) are indicated by the sizes of the arrows in Figure 4.…”

Section: Melatonin Attenuates the Reductions In Energy Pipeline Modelmentioning

confidence: 99%

Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress

Varghese

Alyammahi

Nasreddine

et al. 2019

Plants

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Recent studies have demonstrated melatonin protects various crops against abiotic stresses. However, the effects of melatonin on the photosynthetic apparatus of stressed plants is poorly characterized. We investigated the effects of melatonin pretreatment on photosynthesis and tolerance to salinity stress in Avena sativa (oat) plants. Oat plants were exposed to four treatments (three replicate pots per treatment): well-watered (WW; control); watered with 300 mM salt solution for 10 days (NaCl); pretreated with 100 µM melatonin solution for 7 days then watered normally for 10 days (Mel+W); or pretreated with 100 µM melatonin for 7 days then 300 mM salt for 10 days (Mel+NaCl). Considerable differences in growth parameters, chlorophyll content, stomatal conductance, proline accumulation, lipid peroxidation, electrolyte leakage, and growth parameters were observed between groups. Genes encoding three major antioxidant enzymes were upregulated in the Mel+NaCl group compared to the other groups. Chlorophyll-a fluorescence kinetic analyses revealed that almost all photosynthetic parameters were improved in Mel+NaCl plants compared to the other treatments. Analysis of genes encoding the major extrinsic proteins of photosystem II (PSII) revealed that PsbA, PsbB, PsbC, and PsbD (but not PsbO) were highly upregulated in Mel+NaCl plants compared to the other groups, indicating melatonin positively influenced photosynthesis under control conditions and salt stress. In addition, melatonin upregulated stress-responsive NAC transcription factor genes in plants exposed to salt stress. These findings suggest melatonin pretreatment improves photosynthesis and enhances salt tolerance in oat plants.

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Water stress modulates terpene biosynthesis and morphophysiology at different ploidal levels in Lippia alba (Mill.) N. E. Brown (Verbenaceae)

Lopes,

Nascimento,

Souza

et al. 2023

Protoplasma

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Chlorophyll-a fluorescence evaluation of PEG-induced osmotic stress on PSII activity in Arabidopsis plants expressing SIP1

Cited by 22 publications

References 44 publications

Evaluation of osmotic stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene

Evaluation of osmotic stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene

Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress

Water stress modulates terpene biosynthesis and morphophysiology at different ploidal levels in Lippia alba (Mill.) N. E. Brown (Verbenaceae)

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