Chlorogenic acid‐mediated chemical defence of plants against insect herbivores

Kundu, Anish; Vadassery, Jyothilakshmi

doi:10.1111/plb.12947

Cited by 95 publications

(73 citation statements)

References 42 publications

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“…This metabolite is known to be effective in counteracting the damages of different plant stressors (Yang et al, 2014). Similar accumulation pattern was registered for hydroxypipecolicacid, very recently identified as a mobile signal responsible of the induction of systemic disease resistance in Arabidopsis (Chen et al, 2018); chlorogenic and sinapic acids, which can improve host plants resistance (Nićiforović and Abramovič, 2013; Kundu and Vadassery, 2019); anthranilic acid, precursor of methyl anthranilate, which has been associated with the production of the volatile blend attracting herbivore parasitoids (Köllner et al, 2010).…”

Section: Discussionsupporting

confidence: 64%

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Coppola¹,

et al. 2019

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Beneficial fungi in the genus Trichoderma are among the most widespread biocontrol agents of plant pathogens. Their role in triggering plant defenses against pathogens has been intensely investigated, while, in contrast, very limited information is available on induced barriers active against insects. The growing experimental evidence on this latter topic looks promising, and paves the way toward the development of Trichoderma strains and/or consortia active against multiple targets. However, the predictability and reproducibility of the effects that these beneficial fungi is still somewhat limited by the lack of an in-depth understanding of the molecular mechanisms underlying the specificity of their interaction with different crop varieties, and on how the environmental factors modulate this interaction. To fill this research gap, here we studied the transcriptome changes in tomato plants (cultivar “Dwarf San Marzano”) induced by Trichoderma harzianum (strain T22) colonization and subsequent infestation by the aphid Macrosiphum euphorbiae . A wide transcriptome reprogramming, related to metabolic processes, regulation of gene expression and defense responses, was induced both by separate experimental treatments, which showed a synergistic interaction when concurrently applied. The most evident expression changes of defense genes were associated with the multitrophic interaction Trichoderma -tomato-aphid. Early and late genes involved in direct defense against insects were induced (i.e., peroxidase, GST, kinases and polyphenol oxidase, miraculin, chitinase ), along with indirect defense genes, such as sesquiterpene synthase and geranylgeranyl phosphate synthase . Targeted and untargeted semi-polar metabolome analysis revealed a wide metabolome alteration showing an increased accumulation of isoprenoids in Trichoderma treated plants. The wide array of transcriptomic and metabolomics changes nicely fit with the higher mortality of aphids when feeding on Trichoderma treated plants, herein reported, and with the previously observed attractiveness of these latter toward the aphid parasitoid Aphidius ervi . Moreover, Trichoderma treated plants showed the over-expression of transcripts coding for several families of defense-related transcription factors (bZIP, MYB, NAC, AP2-ERF, WRKY), suggesting that the fungus contributes to the priming of plant responses against pest insects. Collectively, our data indicate that Trichoderma treatment of tomato plants induces transcriptomic and metabolomic changes, which underpin both direct and indirect defense responses.

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Section: Discussionsupporting

confidence: 64%

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Coppola¹,

et al. 2019

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“…This is due to the fact that in 1976, the IUPAC reversed the order of numbering of the carbon atoms in the quinic acid ring and suggested chlorogenic acid (3-CQA) as 5-CQA, despite this, most of the commercial chemical suppliers still use the pre-IUPAC nomenclature. Therefore, in this work, as probably in many others previously published, CGA of C. obtusifolia corresponds actually to 5-CQA isomer considering the correct IUPAC name [ 52 , 53 , 54 , 55 , 56 , 57 , 58 ].…”

Section: Discussionsupporting

confidence: 63%

Integrated Analysis of the Transcriptome and Metabolome of Cecropia obtusifolia: A Plant with High Chlorogenic Acid Content Traditionally Used to Treat Diabetes Mellitus

Cadena-Zamudio

Nicasio-Torres

Monribot‐Villanueva

et al. 2020

IJMS

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This investigation cultured Cecropia obtusifolia cells in suspension to evaluate the effect of nitrate deficiency on the growth and production of chlorogenic acid (CGA), a secondary metabolite with hypoglycemic and hypolipidemic activity that acts directly on type 2 diabetes mellitus. Using cell cultures in suspension, a kinetics time course was established with six time points and four total nitrate concentrations. The metabolites of interest were quantified by high-performance liquid chromatography (HPLC), and the metabolome was analyzed using directed and nondirected approaches. Finally, using RNA-seq methodology, the first transcript collection for C. obtusifolia was generated. HPLC analysis detected CGA at all sampling points, while metabolomic analysis confirmed the identity of CGA and of precursors involved in its biosynthesis. Transcriptome analysis identified differentially expressed genes and enzymes involved in the biosynthetic pathway of CGA. C. obtusifolia probably expresses a key enzyme with bifunctional activity, the hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase and hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HQT/HCT), which recognizes shikimic acid or quinic acid as a substrate and incorporates either into one of the two routes responsible for CGA biosynthesis.

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“…Subclasses include simple phenols, which consist of coumarins and phenolic acids (HCAs and hydroxybenzoic acids) and polyphenols, which consist of flavonoids and tannins [ 13 , 14 , 15 , 16 ]. Several of these secondary stress-related metabolites act as antioxidants [ 17 ] and are associated with anti-microbial defenses or act as chemical deterrents to inhibit attack by insects and grazing animals [ 13 , 18 ]. For example, CGAs serve as precursors for wound-induced polyphenolic barriers such as ligin and suberin [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Profiling of Altered Metabolomic States in Bidens pilosa Leaves in Response to Treatment by Methyl Jasmonate and Methyl Salicylate

Ramabulana

Steenkamp

Madala

et al. 2020

Plants

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Bidens pilosa (Asteraceae) is an edible medicinal plant with many bioactivities reported to have a health-beneficial role in controling various diseases. Though B. pilosa contain a diverse array of natural products, these are produced in relatively low concentrations. A possible way to enhance secondary metabolite production can be through the use of elicitors. Here, the effects of exogenous treatments with two signal molecules—methyl jasmonate (MeJA) and methyl salicylate (MeSA)—on the metabolomic profiles of B. pilosa leaves were investigated. Plants were treated with 0.5 mM of MeJA or MeSA and harvested at 12 h and 24 h. Metabolites were extracted with methanol and separated on an ultra-high performance liquid chromatography system hyphenated to quadrupole time-of-flight mass spectrometry detection. Data was subjected to multivariate statistical analysis and modeling for annotation of metabolites. Hydroxycinnamic acid (HCA) derivatives, such as caffeoylquinic acids (CQAs), tartaric acid esters (chicoric acid and caftaric acid), chalcones, and flavonoids were identified as differentially regulated. The altered metabolomes in response to MeSA and MeJA overlapped to a certain extent, suggestive of a cross-talk between signaling and metabolic pathway activation. Moreover, the perturbation of isomeric molecules, especially the cis geometrical isomers of HCA derivatives by both treatments, further point to the biological significance of these molecules during physiological responses to stress. The results highlight the possibility of using phytohormones to enhance the accumulation of bioactive secondary metabolites in this plant.

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Chlorogenic acid‐mediated chemical defence of plants against insect herbivores

Cited by 95 publications

References 42 publications

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Integrated Analysis of the Transcriptome and Metabolome of Cecropia obtusifolia: A Plant with High Chlorogenic Acid Content Traditionally Used to Treat Diabetes Mellitus

Profiling of Altered Metabolomic States in Bidens pilosa Leaves in Response to Treatment by Methyl Jasmonate and Methyl Salicylate

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