Chlorogenic acid-enriched extract from<i>Eucommia ulmoides</i>leaves inhibits hepatic lipid accumulation through regulation of cholesterol metabolism in HepG2 cells

Hao, Shun; Yuan, Xiutang; Lin, Yunfeng; Mo, Zhentao; Cs, Yang; Peng, Xiaofeng; Xiang, Canhui; Li, Yiqi; Li, Wenna

doi:10.3109/13880209.2015.1029054

Cited by 66 publications

(36 citation statements)

References 27 publications

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“…As to the lipid metabolism influence, chlorogenic acid is even characterized as “an anti-obesity natural molecule” [ 48 ], it is known to reduce hepatic triglycerides level in obese mice (at respectively high dose of 30–60 mg/kg) [ 5 ], and GW tincture exerts such effect in rats receiving ethanol (but at higher dose of 5 ml/kg) [ 49 ]. It has been shown recently that chlorogenic acid is one of the active ingredients in the crude herbal drug counteracting chronic ethanol-induced hepatic lipid accumulation through MAPK/SREBP-1c-dependent and -independent signalling pathways [ 50 ] and in vitro it increases the efflux of total cholesterol and triacylglycerols to the hepatocytes, inhibits HMG-CoA reductase activity [ 51 ]. In addition to chlorogenic acid, other hydroxycinnamic acids may mediate pharmacological activity of GW drugs.…”

Section: Resultsmentioning

confidence: 99%

The influence of goutweed (Aegopodium podagraria L.) tincture and metformin on the carbohydrate and lipid metabolism in dexamethasone-treated rats

Tovchiga

2016

BMC Complement Altern Med

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BackgroundDiabetes mellitus and metabolic syndrome are the common problems of the modern society. The interest in herbal medicines increases, and often they are used in combination with conventional drugs. Aegopodium podagraria L. (goutweed) is a plant widely used in traditional medicine. Hypoglycemic effect of goutweed aerial part tincture has been previously shown in alloxan-induced diabetic mice and in rats receiving excess of fructose and hydrochlorothiazide. The effects of co-administration of the tincture with widely used antihyperglycemic drugs have not been verified. The objective of this study is to determine the efficacy of goutweed tincture and its combination with metformin using the model reproducing the pathogenetic mechanisms of the metabolic syndrome and type 2 diabetes.MethodsThe animals were divided into 5 groups, as follows: intact control, dexamethasone (untreated), dexamethasone + metformin, 50 mg/kg; dexamethasone + A. podagraria tincture, 1 ml/kg intragastrically; dexamethasone + metformin, 50 mg/kg intragastrically + A. podagraria tincture, 1 ml/kg intragastrically. Dexamethasone was used at a dose of 5 mg/kg subcutaneously for 5 days. Insulin tolerance test and oral glucose tolerance test were performed, triglycerides, total lipids, total and HDL cholesterol content in plasma were determined, LDL cholesterol content was calculated, glycogen content in the liver was measured.ResultsGoutweed tincture combined with metformin increased its effect on the basal glycemia and on the results of the short insulin test. In the oral glucose tolerance test the lowest area under glucose curve and average glycemia value were seen in animals receiving this combination. Only metformin tended toward the reduction of liver glycogen. The decrease in triglycerides and increment of HDL cholesterol content (caused by the tincture), as well as tendency towards the decrease in total lipids level (caused by metformin) were observed against a background of the investigated combination, though the ability of GW tincture to reduce LDL cholesterol content and the same tendency seen against a background of metformin were eliminated when these preparations were administered together.ConclusionIt has been shown that goutweed tincture combined with the respectively low dose of metformin partially increases the efficacy of the latter in dexamethasone-treated rats.Graphical abstractGoutweed tincture combined with the respectively low dose of metformin partially increases the efficacy of the latter in dexamethasone-treated rats

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Section: Resultsmentioning

confidence: 99%

The influence of goutweed (Aegopodium podagraria L.) tincture and metformin on the carbohydrate and lipid metabolism in dexamethasone-treated rats

Tovchiga

2016

BMC Complement Altern Med

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“…Lonicera caerulea berry extract has been proven to reduce the cholesterol accumulation by regulating the related gene expression in the liver and the enzymatic activity of hepatic CYP7A1 in rats (Liu et al ., ). Particularly phenolic compounds such as polyphenols, chlorogenic acid, anthocyanins and catechins are generally believed to lower serum cholesterol level, decrease cholesterol and lipid accumulation and maintain the cholesterol balance in human by regulating the expression of cholesterol metabolism‐related miRNAs and activity of lipid metabolism‐related enzymes (Ogino et al ., ; Lee et al ., ; Milenkovic et al ., ; Hao et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Anthocyanin extracts of lingonberry (Vaccinium vitis‐idaea L.) attenuate serum lipids and cholesterol metabolism in HCD‐induced hypercholesterolaemic male mice

Zhang

Zhou

Huangfu

et al. 2018

Int J of Food Sci Tech

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This study is aimed to evaluate the hypocholesterolaemic effect of anthocyanin extracts of lingonberry (LAE) in vitro and vivo studies. In vitro, LAE can effectively inhibit the micellisation of cholesterol with significant dose-effect relationship. In animal experiments, after 10-weeks of LAE administration, lower values of body mass, daily food intake, liver weight, visceral adipose content, TC level, LDL-C level, fasting blood glucose level and higher HDL-C level in serum, cholesterol content and total bile acid content in faeces are observed in HCD-induced hypercholesterolaemic mice compared with model group. These observations suggest that LAE could promote the metabolism of serum cholesterol in HCD-induced hypercholesterolaemic mice primarily via faecal excretion of cholesterol. Furthermore, LAE may reduce the inflammatory cell infiltration and attenuate steatosis and hepatocellular fat deposit in the liver. Our results indicate that LAE is a potential dietary supplement in cholesterol-lowering or for hypercholesterolaemic treatment. These findings may provide important basis for further optimisation of dietary anthocyanins utilisation.

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“…Furthermore, in a very recent paper, Hao et al. 43 showed that the efflux of total cholesterol in HepG2 cells is increased by chlorogenic acid. Accordingly, in our study, an enhanced cholesterol efflux mediated by chlorogenic acid could have prevented the increase in the cholesterol cellular concentration due to the overexpression of HMGR and LDLR induced by quercetin.…”

Section: Resultsmentioning

confidence: 99%

The molecular mechanism of the cholesterol‐lowering effect of dill and kale: The influence of the food matrix components

et al. 2016

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Foods are complex matrices containing many different compounds, all of which contribute to the overall effect of the food itself, although they have different mechanisms of action. While evaluating the effect of bioactive compounds, it is important to consider that the use of a single compound can hide the effects of the other molecules that can act synergistically or antagonistically in the same food. The aim of the present study was to evaluate the influence of food matrix components by comparing two edible plants (dill and kale) with cholesterol‐lowering potential and similar contents of their most representative bioactive, quercetin. The molecular effects of the extracts were evaluated in HepG2 cells by measuring the expression of sterol‐regulatory element‐binding proteins (SREBPs), 3‐hydroxy‐3‐methylglutaryl‐CoA reductase (HMGCR) and low density lipoprotein receptor (LDLR) at the mRNA and protein level. The results reported here show that both extracts reduced the cellular cholesterol level with a similar trend and magnitude. It is conceivable that the slightly different results are due to the diverse composition of minor bioactive compounds, indicating that only by considering food as a whole is it possible to understand the complex relationship between food, nutrition, and health in a foodomics vision.

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Chlorogenic acid-enriched extract fromEucommia ulmoidesleaves inhibits hepatic lipid accumulation through regulation of cholesterol metabolism in HepG2 cells

Abstract: CAEF is a promising dietary supplement to prevent obesity and dyslipidemia and the effects appear to be due, at least in part, to regulating cholesterol metabolism through inhibition of HMGCR in HepG2 cells.

Cited by 66 publications

References 27 publications

The influence of goutweed (Aegopodium podagraria L.) tincture and metformin on the carbohydrate and lipid metabolism in dexamethasone-treated rats

The influence of goutweed (Aegopodium podagraria L.) tincture and metformin on the carbohydrate and lipid metabolism in dexamethasone-treated rats

Anthocyanin extracts of lingonberry (Vaccinium vitis‐idaea L.) attenuate serum lipids and cholesterol metabolism in HCD‐induced hypercholesterolaemic male mice

The molecular mechanism of the cholesterol‐lowering effect of dill and kale: The influence of the food matrix components

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