Chlorogenic acid enhances endothelial barrier function and promotes endothelial tube formation: A proteomics approach and functional validation

A chlorogenic acid functional strategy of anti-inflammation, anti-coagulation and promoted endothelial proliferation for bioprosthetic artificial heart valves

“…Moreover, a large number endothelial cells could be found for all the OX-CO-PP, OX-PB-PP and OX-CA-PP groups with normal stretched and spindle shaped morphology and OX-CA-PP exhibited the best endothelial cell proliferation among all cross-linked PPs. As reported from previous literature studies, chlorogenic acid could promote the release of angiopoietin-2 and formation of eNOS dimers, 31,38 which was beneficial to the proliferation of endothelial cells and triggered the improvement in endothelial cell proliferation for OX-CA-PP.…”

“…[28][29][30] In addition, chlorogenic acid also shows great effects in promoting endothelial cell proliferation by regulating Rap1 and PI3K/AKT signaling pathways and inducing angiopoietin-2 secretion. 31 The excellent bioactive properties of chlorogenic acid perfectly fit with the demands of BHVs.…”

The upregulation of lamin A/C as a compensatory mechanism during tight junction disruption in renal tubular cells mediated by calcium oxalate crystals

“…While the roles for F-actin and α-tubulin and their association with ZO-1 are known to regulate COM-mediated TJ disruption ( Hadpech et al, 2022 , Peerapen and Thongboonkerd, 2021a ), the role of lamin A/C in COM kidney stone model remains unclear. Based on our previous findings ( Pongsakul et al, 2016 , Semangoen et al, 2008 ) and recent studies ( Karakaya et al, 2022 , Wuttimongkolchai et al, 2022 ), we hypothesized that lamin A/C might be associated with ZO-1 and its upregulation might be related to the COM-induced TJ disruption (either as the cause or the effect). The present study, therefore, addressed the roles for lamin A/C and its association with ZO-1 in COM-mediated TJ disruption.…”

“…A functional study has revealed that knockdown of LMNA gene encoding lamin A/C can suppress expression of several COM crystal receptors on the cell membranes, thereby reducing crystal-cell adhesion ( Pongsakul et al, 2016 ). Recent evidence has also shown that infectious and chemical stimuli cause changes in lamin A/C together with alterations in TJ complex and receptors on the cell membranes in endothelial cells ( Karakaya et al, 2022 , Wuttimongkolchai et al, 2022 ), suggesting association among lamin A/C, TJ complex, and the cell membranes. While the roles for F-actin and α-tubulin and their association with ZO-1 are known to regulate COM-mediated TJ disruption ( Hadpech et al, 2022 , Peerapen and Thongboonkerd, 2021a ), the role of lamin A/C in COM kidney stone model remains unclear.…”

Bioactive functions of chlorogenic acid and its research progress in pig industry

“…Similarly, coffee with a high content of CGA improved postprandial endothelial dysfunction in patients with borderline and stage 1 hypertension by improving postprandial flow‐mediated vasodilation and decreasing circulating 8‐isoprostane levels (Kajikawa et al, 2019). A recent report revealed that CGA enhanced endothelial barrier function and promoted endothelial tube formation, which was related to CGA‐enhanced capillary‐like endothelial tube formation and secretion of angiopoietin‐2 (Wuttimongkolchai et al, 2022).…”