Chlorogenic Acid Enhances Abdominal Skin Flap Survival Based on Epigastric Artery in Nondiabetic and Diabetic Rats

Bağdaş, Deniz; Etoz, Betul Cam; Gül, Zülfiye; Özyiğit, M. Özgür; Çinkılıç, Nilüfer; Inan, Sevda; Büyükcoşkun, Naciye Işbil; Ozluk, Kasim; Gurun, Mine Sibel

doi:10.1097/sap.0000000000000313

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…These compounds have therapeutic effects against diabetic complications such as diabetic retinopathy 10 . Specifically, several studies have reported that chlorogenic acid has a protective effect on skin wound healing in DM 12, 18, 19. To our knowledge, no study has reported the effects of 3,5-di-O-caffeoylquinic acid on diabetic wound healing.…”

Section: Discussionmentioning

confidence: 99%

Aster koraiensis extract improves impaired skin wound healing during hyperglycemia

Hyun¹,

Kim²,

Jo³

et al. 2018

Integrative Medicine Research

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BackgroundDiabetes mellitus (DM) is one of the most common diseases found across the world. Aster koraiensis extract (AKE) has a protective effect on diabetic complications such as diabetic retinopathy. However, the effects of AKE on hyperglycemia-linked impairment of wound healing during DM have not been elucidated. In this study, we investigated the effects of AKE on delayed wound healing induced by DM.MethodsDM was induced by intraperitoneal administration of streptozotocin (STZ; 75 mg/kg) to Sprague Dawley (SD) rats. Next, a wound was induced on the back of rats after administration of STZ. Further, AKE was prepared using an alcoholic extraction of A. koraiensis and orally administered daily for 18 days. Wound healing was evaluated using an in vitro migration assay and measuring the wound area in vivo. Skin tissue thickness was evaluated using hematoxylin and eosin staining. Matrix metalloprotease (MMP) activity and expression were detected using zymography and immunohistochemistry.ResultsAKE administration improved the delayed migration of keratinocytes in hyperglycemic animals. It also attenuated an increase in keratinocyte MMP-2/9 activity induced by hyperglycemia. AKE protected against DM-induced impaired wound healing in rats and prevented the degradation of skin tissue induced by DM. In addition, AKE attenuated DM-induced increase in MMP-2/9 expression in skin tissue.ConclusionsIn conclusion, AKE may promote wound healing by re-epithelization via promotion of keratinocyte migration and by attenuating the disruption of the skin tissue layer via MMP-2/9 inhibition during hyperglycemia.

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Section: Discussionmentioning

confidence: 99%

Aster koraiensis extract improves impaired skin wound healing during hyperglycemia

Hyun¹,

Kim²,

Jo³

et al. 2018

Integrative Medicine Research

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show abstract

“…Diabetes was induced by an intraperitoneal application of 50 mg/ kg of streptozotocin (STZ; Solarbio, Beijing, China), as previously described. 29,30 The STZ was dissolved in a vehicle (citrate buffer, 0.1 M, pH 4.5; Sigma, St. Louis, MO). The control group was injected with an equivalent volume of citrate buffer.…”

Section: Methodsmentioning

confidence: 99%

“…25 Flap surgery under diabetic conditions results in numerous pathophysiological problems, such as flap necrosis. 26 Diabetes is also a major risk factor for poor patency of venous anastomoses. A high systemic glucose level ( > 165 mg/dl) increases the rate of flap venous compromise.…”

mentioning

confidence: 99%

Continuous Interstitial Glucose Measurement for Flap Venous Occlusion Monitoring in a Diabetic Model

Zhang

Wang

et al. 2022

Journal of Craniofacial Surgery

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Glucose has been used in the monitoring of transplanted flaps. However, few studies have focused on the effect of diabetes on the decrease in flap glucose levels. The purpose of this study was to evaluate the application of continuous interstitial glucose monitoring to detect flap venous occlusion in a diabetic rat model. The flap interstitial glucose level was measured by transcutaneous sensors in the bilateral vertical rectus abdominis myocutaneous flaps of each rat. interstitial glucose values in the bilateral flaps were recorded every 5 minutes after the venous occlusion of 1 flap. Forty-one rats were divided into the control group (n = 13), diabetic group (n = 15, established by the intraperitoneal injection of streptozotocin), and insulintreated diabetic group (n = 13, systemic glucose well controlled by insulin before surgery). Results indicated that flap interstitial glucose decreased to the lowest level in all flaps with venous occlusion. The time from venous occlusionto the lowest interstitial glucose level was significantlylongerinthe diabetic group than the control group (120.8 ± 5.7 minutes versus 56.5 ± 6.7 min-minutes, P < 0.001), but was similar in the insulintreated diabetic and control groups (56.5 ± 6.7 minutes versus 56.0 ± 6.6 minutes, P = 0.876). The change in interstitial glucose in both the diabetic and insulin-treated diabetic groups began with a plateau period or a slow decline before decreasing for 49.0 ± 6.4 minutes and 24.4 ± 4.3 min-minutes, respectively. in a diabetic rat model, impaired glucose metabolism prolongs the required monitoring time and dulls the reaction of flap interstitial glucose to venous congestion. Insulin restores the prolonged monitoring time, but not the reaction of flap interstitial glucose. Therefore, care is needed during application of the technique in diabetic patients.

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“…Previous studies on skin flap survival [15,16] performed general histological scoring and semi-quantitative analysis. To comprehensively assess the histology, we chose 5 parameters (fiber structure, fiber arrangement, inflammation, vascularity, and density of cells) based on HE staining.…”

Section: Methodsmentioning

confidence: 99%

Effect of Autologous Platelet-Rich Plasma Gel on Skin Flap Survival

Chai

Zou

2019

Med Sci Monit

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Background Skin flap grafting is one of the most common tissue transplantations for wound repair and organ reconstruction. Thus, improving the survival rate of the transplanted skin flap is important. Platelet-rich plasma (PRP) is an autologous platelet concentrate obtained from whole blood. It has been widely used in repairing tissue defects. Considering that the PRP gel has similar biological characteristics, this study used PRP gel for skin flap transplantation. Material/Methods PRP gel from Sprague-Dawley (SD) rats was prepared and the growth factor concentration was determined. A rat skin flap model was established to evaluate the survival rate of skin flap. Morphologic evaluation was also done. Results We found that the PRP gel increased the survival rate of the skin flap. In addition, it reduces the inflammation response in skin flap transplantation and has better effects in terms of generating new soft tissue. Conclusions The effectiveness PRP gel in skin flap transplantation is satisfactory. The possible mechanisms by which PRP gel promotes the survival of the skin flap includes platelets, growth factors, immune activity factor, and fibrin. PRP could be a new clinical method for promoting skin flap survival.

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Chlorogenic Acid Enhances Abdominal Skin Flap Survival Based on Epigastric Artery in Nondiabetic and Diabetic Rats

Cited by 10 publications

References 26 publications

Aster koraiensis extract improves impaired skin wound healing during hyperglycemia

Aster koraiensis extract improves impaired skin wound healing during hyperglycemia

Continuous Interstitial Glucose Measurement for Flap Venous Occlusion Monitoring in a Diabetic Model

Effect of Autologous Platelet-Rich Plasma Gel on Skin Flap Survival

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