Chlorogenic Acid Alleviates Hyperglycemia‐Induced Cardiac Fibrosis through Activation of the NO/cGMP/PKG Pathway in Cardiac Fibroblasts

Qin, Linhui; Zang, Ming-Xi; Xu, Yan; Zhao, Rongrong; Wang, Yating; Mi, Yang; Mei, Yingwu

doi:10.1002/mnfr.202000810

Cited by 32 publications

(27 citation statements)

References 51 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At last, it was possible that other pathways had an important influence on the progression of ASFV entry via some biological process, such as cGMP-PKG signaling pathway, cAMP signaling pathway, and AMPK signaling pathway, which were screened out by KEGG analysis. cGMP is the intracellular second messenger that mediates the action of nitric oxide (NO) and natriuretic peptides (NPs), affecting a wide range of physiologic processes ( 22 ). cGMP/PKG signaling pathway was associated with the replication of some viruses ( 23 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus In Vitro

et al. 2021

View full text Add to dashboard Cite

African swine fever virus (ASFV) has been identified as the agent of African swine fever, resulting in a mortality rate of nearly 100% in domestic pigs worldwide. Protein p22 encoded by gene KP177R has been reported to be localized at the inner envelope of the virus, while the function of p22 remains unclear. In this study, p22 interacting proteins of the host were identified by a high-throughput method and analyzed by Gene ontology terms and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways; numerous cellular proteins in 293-T that interacted with p22 protein were identified. These interacting proteins were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc. At the same time, the interacted proteins participated in several KEGG pathways like ribosome, spliceosome, etc. The key proteins in the protein–protein interaction network were closely related to actin filament organization and movement, resulting in affecting the process of phagocytosis and endocytosis. A large number of proteins that interacted with p22 were identified, providing a large database, which should be very useful to elucidate the function of p22 in the near future, laying the foundation for elucidating the mechanism of ASFV.

show abstract

Section: Discussionmentioning

confidence: 99%

A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus In Vitro

et al. 2021

View full text Add to dashboard Cite

show abstract

“…CGA administration attenuated vascular senescence in a dose-dependent manner through the Nrf2/HO-1 pathway and the increase of eNOS expression in an in vivo model [ 39 ]. In Type I diabetic mice, CGA treatment increased cyclic GMP (cGMP) level and activated protein kinase G (PKG) in cardiac fibroblasts by enhancing eNOS activity and NO production, thereby contributing to the inhibition on cardiac fibrosis caused by hyperglycemia [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Glossogyne tenuifolia Extract Increases Nitric Oxide Production in Human Umbilical Vein Endothelial Cells

et al. 2021

View full text Add to dashboard Cite

The vascular nitric oxide (NO) system has a protective effect in atherosclerosis. NO is generated from the conversion of L-arginine to L-citrulline by the enzymatic action of endothelial NO synthase (eNOS). Compounds with the effect of enhancing eNOS expression are considered to be candidates for the prevention of atherosclerosis. In this study, extracts from the aerial, root, and whole plant of Glossogyne tenuifolia (GT) were obtained with ethanol, n-hexane, ethyl acetate (EA), and methanol extraction, respectively. The effects of these GT extracts on the synthesis of NO and the expression of eNOS in human umbilical vein endothelial cells (HUVECs) were investigated. NO production was determined as nitrite by colorimetry, following the Griess reaction. The treatment of HUVECs with EA extract from the root of GT and n-hexane, methanol, and ethanol extract from the aerial, root, and whole plant of GT increased NO production in a dose-dependent manner. When at a dose of 160 μg/mL, NO production increased from 0.9 to 18.4-fold. Among these extracts, the methanol extract from the root of GT (R/M GTE) exhibited the most potent effect on NO production (increased by 18.4-fold). Furthermore, using Western blot and RT–PCR analysis, treatment of HUVECs with the R/M GTE increased both eNOS protein and mRNA expression. In addition, Western blot analysis revealed that the R/M GTE increased eNOS phosphorylation at serine1177 as early as 15 min after treatment. The chemical composition for the main ingredients was also performed by HPLC analysis. In conclusion, the present study demonstrated that GT extracts increased NO production in HUVECs and that the R/M GTE increased NO production via increasing eNOS expression and activation by phosphorylation of eNOS at serine1177.

show abstract

“…At last, it was possible that other pathways had an important in uence on the progression of ASFV entry via some biological process, such as cGMP-PKG signaling pathway, cAMP signaling pathway and AMPK signaling pathway which were screened out by KEGG analysis. cGMP is the intracellular second messenger that mediates the action of nitric oxide (NO) and natriuretic peptides (NPs), affecting a wide range of physiologic processes [21]. cAMP is also one of the most common and universal second messengers, cAMP regulates pivotal physiologic processes including metabolism, secretion, calcium homeostasis, muscle contraction, cell fate, and gene transcription [22].…”

Section: Discussionmentioning

confidence: 99%

A High Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus in Vitro

Zhu

Fan

Zhou

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: African Swine Fever Virus (ASFV) has been identified as the agent of ASF, which has resulting in a mortality rate of nearly 100% in domestic pigs worldwide. Protein p22 encoded by pKP177L was reported to be localized at the inner envelope of the virus, while the function of p22 remains unclear.Methods: Protein p22 interacted proteins of the host were immune-precipitated and identified by Liquid Chromatography Mass Spectrometry, analyzed by Go terms and KEGG pathways. Results: Numerous cellular proteins in 293-T that interacted with p22 protein were identified. These interacted proteins were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. At the same time, the interacted proteins participated in several KEGG pathways like ribosome, splicesome, etc. The key proteins in PPI network were closely related to actin filament organization and movement, resulting in affecting the process of phagocytosis and endocytosis. Conclusions: The identified high number of proteins interacted with p22 and as a large database should be very useful to elucidate the function of p22 in the near future, and lay the foundation for elucidating the mechanism of ASFV.

show abstract

Chlorogenic Acid Alleviates Hyperglycemia‐Induced Cardiac Fibrosis through Activation of the NO/cGMP/PKG Pathway in Cardiac Fibroblasts

Cited by 32 publications

References 51 publications

A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus In Vitro

A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus In Vitro

Glossogyne tenuifolia Extract Increases Nitric Oxide Production in Human Umbilical Vein Endothelial Cells

A High Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus in Vitro

Contact Info

Product

Resources

About