Chlorogenic acid, a polyphenolic compound, treats mice with septic arthritis caused by Candida albicans

Lee, Jue-Hee; Park, Ji Hye; Kim, Yeong Shik; Ye, Han

doi:10.1016/j.intimp.2008.08.002

Cited by 42 publications

(26 citation statements)

References 30 publications

(36 reference statements)

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“…An arthritis was induced as previously described (Han, 2005;Lee et al, 2008). In brief, the CACW (400 µg) dissolved in sterile 1 mL DPBS was mixed with 1 mL Complete Freund's Adjuvant (CFA; Difco) at a 1:1 ratio by volume.…”

Section: Induction Of Cacw-induced Arthritismentioning

confidence: 99%

“…The lonicerin effect on T-cells proliferation was done by following the procedures previously described (Lee et al, 2008;Han, 2009). In brief, splenocytes were harvested from BALB/c mouse strain through an aseptic procedure, incubated at 35 o C-5% CO 2 for 24 h, and plated in 96-well tissue culture plates (Becton Dickinson) at 5 × 10 5 /well with a final concentration of 5 µg concanavalin A (Con A; C2010, Sigma-Aldrich).…”

Section: Lonicerin Effects On T-cells Proliferation and Cytokine Prodmentioning

confidence: 99%

“…These experiments were done by using a murine macrophage cell line RAW 264.7 (Korean Cell Line Bank) as previously described (Han, 2005;Lee et al, 2008). To 100 µL of macrophages (2 × 10 5 cells/mL) put in 96-well plated (Becton Dickinson), a fixed amount of lipopolysaccharide (LPS; 1 µg/mL; E. coli 026:B6; Sigma) and various doses (0, 2.5, 5, 10, 20, and 40 µg/mL) of lonicerin were simultaneously added to a designated well.…”

Section: Lonicerin Activity On Macrophagesmentioning

confidence: 99%

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Antiarthritic effect of lonicerin on Candida albicans arthritis in mice

Lee

2011

Arch. Pharm. Res.

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Fungal arthritis is a potentially serious disease resulting in rapid destruction of the joint. Among the various Candida species, Candida albicans is the most commonly associated with fungal arthritis. In the present study, we examined the effect of lonicerin, a flavonoid isolated from Lonicerae Flos, on an arthritis caused by C. albicans cell wall (CACW) in mice. To examine the effect, an emulsified mixture of CACW and complete Freund's adjuvant (CACW/CFA) was injected into BALB/c mice via hind footpad route on days -3, -2, and -1. On Day 0, mice with the swollen footpad received lonicerin at 1 or 2 mg/dose/time intraperitoneally 3 times every other day. The footpad-swelling was measured for 20 days. Results showed that the lonicerin treatment reduced the edema at all dose levels, and, furthermore, there was app. 54% edema reduction in animals given the 2 mg-dose at the peak (day 10) of septic arthritis (p < 0.05). Since the peak, the edema was reduced in similar rates. This antiarthritic activity appeared to be mediated by lonicerin's ability to suppress T cell proliferation, nitric oxide production from macrophages, and shift of cellular immunity from Th1- toward Th2-type responses, all of which are beneficial to treat arthritis. In addition, the flavonoid had anticandidal activity (p < 0.01). These data suggest that lonicerin alone, which has both anti-arthritic and antifungal activities, can result in a combination therapy for the treatment of fungal arthritis due to C. albicans infection.

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Section: Induction Of Cacw-induced Arthritismentioning

confidence: 99%

Section: Lonicerin Effects On T-cells Proliferation and Cytokine Prodmentioning

confidence: 99%

Section: Lonicerin Activity On Macrophagesmentioning

confidence: 99%

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Antiarthritic effect of lonicerin on Candida albicans arthritis in mice

Lee

2011

Arch. Pharm. Res.

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“…The CACW contains the most antigenic sources responsible for pathogenesis of candidiasis (Han, 2005(Han, , 2009. The induction was induced as previously described (Han, 2005(Han, , 2009Lee et al, 2008). In brief, CACW (400 µg) dissolved in sterile DPBS was mixed with Complete Freund's Adjuvant (CFA; Difco) at a 1:1 ratio by volume.…”

Section: Induction Of Fungal Septic Arthritismentioning

confidence: 99%

“…Prior to these experiments, Och activity on mouse splenocytes stimulated with Con A was examined as previously described (Lee et al, 2008;Han, 2009). In addition, effect of Och on T cell activation, in the same experimental design as described above, was done with mouse splenocytes.…”

Section: Och Activity On T Cellsmentioning

confidence: 99%

Involvement of T-cell immunoregulation by ochnaflavone in therapeutic effect on fungal arthritis due to Candida albicans

Lee

2011

Arch. Pharm. Res.

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Arthritis due to pathogenic fungi is a serious disease causing rapid destruction of the joint. In the pathogenesis of arthritis, T lymphocytes are considered to be one of the major immune cells. In present study, we examined the T cell immunoregulatory effect by ochnaflavone (Och), a biflavonoid, on arthritis caused by Candida albicans that is the most commonly associated with fungal arthritis. To examine the effects of ochnaflavonon Candida albicans-caused septic arthritis, an emulsified mixture of C. albicans cell wall and complete Freund's adjuvant (CACW/CFA) was injected into BALB/c mice via hind footpad route on days -3, -2, and -1. On Day 0, Och at 1 or 2 mg/dose/time was intratraperitoneally given to mice with the swollen footpad every other day for 3 times. The footpad-edema was measured for 20 days. Results revealed that Och reduced the edema at all dose levels and furthermore, there was app. 45% reduction of the edema in animals given 2 mg-dose at the peak of septic arthritis (p < 0.05). This anti-arthritic effect was accompanied by the diminishing of the DTH (delayed type hypersensitivity) activity against the CACW and by the provoking of the dominant T helper 2 (Th2) type cytokines production (IL-4 and Il-10), which appeared to result in a suppression of T helper 1 cytokines (IFN-γ and IL-2). Besides the T cell immunoregulatory activity, Och inhibited T cells activation as evidenced by the IL-2 reduction from PMA/ionomycin-stimulated Jurkat cell line and in addition, the compound killed macrophages in a dose-dependent manner (p < 0.05). However, Och caused no hemolysis (p < 0.05). These data implicate that Och, which has anti-arthritic activity based on the Th2 dominance as well as macrophage removal, can be safely administered into the blood circulation for treatment of the arthritis caused by C. albicans. Thus, it can be concluded that Och would be an ideal immunologically evaluated agent for treating of Candida arthritis.

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