Chloroform induces cystein-rich 61, a mediator of collagen homeostasis via early growth response-1 dependent pathway in human skin dermal fibroblasts

Lee, Hana; Park, Jae Beom; Ryu, Woo In; Kim, Ji Hyun; Shin, Jung Jin; Son, Sang Wook

doi:10.1007/s13273-016-0038-6

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…Collagen is derived from precursor procollagen that includes the extra‐peptide sequences in N and C‐terminus (Seo et al., ). After cleavage of this additional peptide sequence from procollagen by procollagen peptidase, collagens are synthesized as a kind of components for extracellular matrix proteins and incorporated into mesh network of extracellular fibrils (Lee, Hossaine, et al., ; Lee, Park, et al., ). To examine the amount of collagen in CCD986Sk cell culture media treated by the ethanol extract of L. cuneata G. Don, the level of Type I collagen was measured by procollagen Type I C‐peptide ELISA assay kit as the amount of procollagen secreted from CCD986Sk cell reflects the amount of collagen.…”

Section: Resultsmentioning

confidence: 99%

“…Lespedeza cuneata G. Don belongs to Lespedeza and is widely distributed in Japan, China, Taiwan, and Korea (Han, Chung, Nemoto, & Choi, ). The biological activity of L. cuneata G. Don has been elucidated by several reports demonstrating antiendothelial dysfunction induced by methylglyoxal glucotoxicity (Do et al., ), antiaging activity (Seong et al., ), anti‐inflammation activity (Lee, Hossaine, & Park, ; Lee, Park, et al., ), hepatoprotective effect (Kim et al., ), and antinitric oxide production activity (Yoo et al., ). As a physiologically active substance of L. cuneata G. Don, β‐sitosterol, quercetin, kaempferol, pinitol, avicularin, juglanin, and trifolin (Matsuura, Iinuma, Ito, Takami, & Kagei, ) have been identified.…”

Section: Introductionmentioning

confidence: 99%

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Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients

Lee

Park

2018

Food Science & Nutrition

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To develop the ingredient with the cosmeceutical function, the antiwrinkle and antimelanogenesis effects of the ethanol extract of Lespedeza cuneata G. Don were investigated. DPPH radical scavenging activity was significantly increased with the extract of L. cuneata G. Don. Cell viability on CCD986Sk human fibroblast was also increased by the ethanol extract of L. cuneata G. Don. The inhibitory function of the extract of L. cuneata G. Don on collagenase, elastase, and tyrosinase was evaluated. Protein expression level of Claudin‐1, Occludin, and ZO‐1 was up‐regulated in HaCaT human keratinocyte by the extract of L. cuneata G. Don. In addition, the extract of L. cuneata G. Don inhibited melanin synthesis in B16F10 murine melanoma cells by decreasing MITF, TRP1, and TRP2 protein levels and increasing the phosphorylated Erk and Akt. Thus, these findings would be useful for developing the new cosmeceutical formulations based on the extract of L. cuneata G. Don.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients

Lee

Park

2018

Food Science & Nutrition

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“…The human keratinocyte (HaCaT) and human fibroblast cell lines (Hs68) were obtained from the American Type Culture Collection (ATCC). The cells were incubated in complete Dulbecco’s modified Eagle’s medium (DMEM; Hyclone, Logan, UT, USA) containing 100 U/mL penicillin, streptomycin (100 μg/mL), and 10% fetal bovine serum (FBS) at 37°C [ 24 , 25 ]. Primary human sebocytes (Celprogen Inc., USA) were maintained in Human Sebocyte Complete Growth Media purchased from the same vendor.…”

Section: Methodsmentioning

confidence: 99%

“…Proteins were separated by 10% SDS-PAGE, and transferred onto polyvinylidene fluoride membrane (Bio-Rad Laboratories, CA) [ 28 ]. After blocking for 2 h at room temperature, the membranes were incubated overnight at 4°C with primary antibodies against iNOS, IL-1β, COX-2, IKKα, IKKβ, p-IKKα/β, IκBα, p-IκBα, NF-κB p65, p-NF-κB p65, p38, p-p38, ERK, p-ERK, JNK, p-JNK, AKT, p-AKT, mTOR, p-mTOR, PPARγ, FAS, AMPKα, p-AMPKα, FoxO1, and p-FoxO1 (Cell Signaling Technology, USA), SREBP1 (Novus Biologicals, Canada) and β-actin (Santa Cruz Biotechnology, USA) which were diluted using manufacturers’ recommendations [ 25 , 29 ]. The membranes were then washed in 1× TBST and incubated with the appropriate secondary antibody HRP-conjugated (1:5000) at room temperature for 1 h. Protein bands were visualized using the Sensi-Q 2000 (Lugen, South Korea).…”

Section: Methodsmentioning

confidence: 99%

The ameliorative effect of hemp seed hexane extracts on the Propionibacterium acnes-induced inflammation and lipogenesis in sebocytes

Jin

Lee

2018

PLoS ONE

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In this study, we investigated the anti-microbial, anti-inflammatory, and anti-lipogenic effects of hemp (Cannabis sativa L.) seed hexane extracts, focusing on the Propionibacterium acnes-triggered inflammation and lipogenesis. Hemp seed hexane extracts (HSHE) showed anti-microbial activity against P. acnes. The expression of iNOS, COX-2, and the subsequent production of nitric oxide and prostaglandin increased after infection of P. acnes in HaCaT cells, however, upon treating with HSHE, their expressions were reduced. P. acnes-induced expressions of IL-1β and IL-8 were also reduced. HSHE exerted anti-inflammatory effects by regulating NF-κB and MAPKs signaling and blunting the translocation of p-NF-κB to the nucleus in P. acnes-stimulated HaCaT cells. Moreover, P. acnes-induced phosphorylation of ERK and JNK, and their downstream targets c-Fos and c-Jun, was also inhibited by HSHE. In addition, the transactivation of AP-1 induced by P. acnes infection was also downregulated by HSHE. Notably, HSHE regulated inflammation and lipid biosynthesis via regulating AMPK and AKT/FoxO1 signaling in IGF-1-induced inflammation and lipogenesis of sebocytes. In addition, HSHE inhibited 5-lipoxygenase level and P. acnes-induced MMP-9 activity, and promoted collagen biosynthesis in vitro. Thus, HSHE could be utilized to treat acne vulgaris, through its anti-microbial, anti-inflammatory, anti-lipogenic, and collagen-promoting properties.

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“…Western blot analyses were performed as described previously (Lee et al, 2016). Briefly, prepared skin tissues were cut into pieces and homogenized with the Bullet Blender homogenization kit (Next Advance Inc., Averill Park, NY, USA).…”

Section: Western Blot Analysismentioning

confidence: 99%

Taeumjowi-tang, a Traditional Korean Sasang Remedy, Improves Obesity-Atopic Dermatitis Comorbidity by Regulating Hypoxia-Inducible Factor 1 Alpha

et al. 2019

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Atopic dermatitis (AD) is an inflammatory disease of the skin, resulting from an immune dysfunction, that often occurs as a comorbidity of obesity. This investigation evaluated the capacity of Taeumjowi-tang (TJT), a Korean herbal formulation from the Sasang medical tradition to influence prognostic features of AD and obesity in a mouse model. Here, obesity and AD were induced by a high-fat diet (HFD) and 1-fluoro-2,4-dinitrobenzene (DNFB). Following an 8-week HFD regimen and 4 weeks of DNFB administration, the comorbid (CO) group manifested increased body weight and AD-like lesions, as compared to normal control (NC) mice, while TJT administration diminished these symptoms of obesity and AD. Specifically, TJT treatment reduced epidermal thickness and eosinophil/mast cell infiltration, along with reduction in immunoglobulin E, interleukin (IL)-4, IL-6, and tumor necrosis factor-alpha (TNF-a). It was additionally demonstrated that TJT suppresses HFD/DNFB-associated increase of the inflammation-related nuclear factor-kappa beta (NF-kB) and mitogen activated protein kinase. Moreover, significantly increased levels of hypoxia inducible factor-1 alpha (HIF-1a) protein was observed in CO group versus controls, an increase significantly down-regulated by TJT-treatment. These outcomes suggest that TJT may prove useful in clinical management of obesity-AD comorbidity treatment, an effect that may be due to regulation of HIF-1a expression.

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Chloroform induces cystein-rich 61, a mediator of collagen homeostasis via early growth response-1 dependent pathway in human skin dermal fibroblasts

Cited by 7 publications

References 33 publications

Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients

Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients

The ameliorative effect of hemp seed hexane extracts on the Propionibacterium acnes-induced inflammation and lipogenesis in sebocytes

Taeumjowi-tang, a Traditional Korean Sasang Remedy, Improves Obesity-Atopic Dermatitis Comorbidity by Regulating Hypoxia-Inducible Factor 1 Alpha

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