Chloroform Cometabolism by Butane-Grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b

Hamamura, Natsuko; Page, Cynthia; Long, Tulley; Semprini, Lewis; Arp, Daniel J.

doi:10.1128/aem.63.9.3607-3613.1997

Cited by 94 publications

(54 citation statements)

References 32 publications

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“…Although chloroform-degrading microbes were found as early as in the 1990s, known chloroform degradation pathways were mostly limited to aerobically co-metabolic processes, which required primary substrates such as short-chain alkanes (Hamamura et al, 1997;Frascari al., 2005), toluene (McClay et al, 1996) or methanol (Bagley and Gossett, 1995) to boost the growth of degraders. In this study, isolate Desulfitobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

A Desulfitobacterium sp. strain PR reductively dechlorinates both 1,1,1‐trichloroethane and chloroform

Ding

Zhao

2014

Environmental Microbiology

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1,1,1-Trichloroethane (TCA) and chloroform are two notorious groundwater pollutants. Here we report the isolation and characterization of Desulfitobacterium sp. strain PR that rapidly dechlorinates both compounds. In pyruvate-amended medium, strain PR reductively dechlorinates ∼ 1.0 mM TCA completely to monochloroethane within 15 days. Under the same conditions, strain PR dechlorinates ∼ 1.2 mM chloroform to predominantly dichloromethane (∼ 1.14 mM) and trace amount of monochloromethane (∼ 0.06 mM) within 10 days. Strain PR shares 96.7% 16S rRNA gene sequence similarity with its closest relative - Desulfitobacterium metallireducens strain 853-15; however, it distinguishes itself from known Desulfitobacterium strains by its inability of utilizing several of their commonly shared substrates such as lactate, thiosulfate and sulfite. A reductive dehalogenase gene (ctrA) in strain PR was identified to be responsible for dechlorination of both TCA and chloroform, showing a maximum expression level of 5.95 ∼ 6.25 copies of transcripts cell(-1) . CtrA shares 94% amino acid sequence identity with CfrA in Dehalobacter sp. strain CF50 and DcrA in Dehalobacter sp. strain DCA. Interestingly, strain PR could tolerate high aqueous concentrations (up to 0.45 mM) of trichloroethene, another groundwater pollutant that often coexists with TCA/chloroform. As the first chloroform-respiring and the second TCA-respiring isolate that has been identified, Desulfitobacterium sp. strain PR may prove useful in remediation of halogenated alkanes with trihalomethyl (-CX₃) groups.

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Section: Discussionmentioning

confidence: 99%

A Desulfitobacterium sp. strain PR reductively dechlorinates both 1,1,1‐trichloroethane and chloroform

Ding

Zhao

2014

Environmental Microbiology

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“…This strain expresses soluble butane monooxygeanse (sBMO) to oxidize butane to 1-butanol (Arp, 1999), which is further converted to butyraldehyde, and then to butyrate (Vangnai et al, 2002). Previous studies also showed that sBMO can degrade a wide range of chlorinated alkanes and aromatics via cometabolic reactions (Doughty et al, 2005;Halsey et al, 2005;Hamamura et al, 1997). P. oleovorans is a soil isolate known for its ability to express alkane monooxygenses to degrade octane to octanoic acid (Baptist et al, 1963) and to oxidize various C 6 -C 12 linear alkanes or alkenes (Witholt et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Biodefluorination and biotransformation of fluorotelomer alcohols by two alkane‐degrading Pseudomonas strains

Kim¹,

Wang²,

McDonald³

et al. 2012

Biotech & Bioengineering

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Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTOHs by two well-studied Pseudomonas strains: Pseudomonas butanovora (butane oxidizer) and Pseudomonas oleovorans (octane oxidizer). Both strains could defluorinate 4:2, 6:2, and 8:2 FTOHs, with a higher degree of defluorination for 4:2 FTOH. According to the identified metabolites, P. oleovorans transformed FTOHs via two pathways I and II. The pathway I led to the production of x:2 ketone [dominant metabolite, F(CF(2))(x)C(O)CH(3); x = n - 1, n = 6 or 8], x:2 sFTOH [F(CF(2))(x)CH(OH)CH(3)], and perfluorinated carboxylic acids (PFCAs, perfluorohexanoic, or perfluorooctanoic acid). The pathway II resulted in the formation of x:3 polyfluorinated acid [F(CF(2))(x) C(2)CH(2) COOH] and relatively minor shorter-chain PFCAs (perfluorobutyric or perfluorohexanoic acid). Conversely, P. butanovora transformed FTOHs by using the pathway I, leading to the production of x:2 ketone, x:2 sFTOH, and PFCAs. This is the first study to show that individual bacterium can bio-transform FTOHs via different or preferred transformation pathways to remove multiple --CF(2) -- groups from FTOHs to form shorter-chain PFCAs.

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“…Under aerobic conditions biodegradation of CF is poor (Bouwer et al, 1981a,b). Appreciable rates of aerobic CF biodegradation only occur during oxidative cometabolism with other primary substrates such as methane (Alvarez-Cohen and Speitel, 2001), butane (Hamamura et al, 1997), or toluene (McClay et al, 1996). Alternatively, CF is reductively dechlorinated under anaerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

Riboflavin‐ and cobalamin‐mediated biodegradation of chloroform in a methanogenic consortium

Guerrero–Barajas

Field

2005

Biotech & Bioengineering

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Chloroform (CF) is an important priority pollutant contaminating groundwater. Reductive dechlorination by anaerobic microorganisms is a promising strategy towards the remediation of CF. The objective of this study was to evaluate the use of redox active vitamins as electron shuttles to enhance the anaerobic biodegradation of CF in an unadapted methanogenic consortium not previously exposed to chlorinated compounds. Only negligible degradation of CF was observed in control cultures lacking redox active vitamins. The addition of riboflavin (RF), cyanocobalamin (CNB12), and hydroxycobalamin (HOB12) enabled biodegradation of CF. The reactions were predominantly catalyzed biologically as evidenced by the lack of any CF conversion in heat-killed controls amended with the cobalamins or minor conversion with RF. In live cultures, significant increases in the rate of CF conversion was observed at substoichiometric molar ratios as low as 0.1 to 0.01 vitamin:CF for RF and CNB12, respectively. At the highest molar vitamin:CF ratios tested of 0.2, the first-order rate constant of CF degradation was 5.3- and 91-fold higher in RF and CNB12 amended cultures, respectively, compared to the unamended control culture. The distribution of biotransformation products was highly impacted by the type of redox active vitamin utilized. Cultures supplemented with RF provided high yields of dichloromethane (DCM). On the other hand, cobalamins promoted the near complete mineralization of organochlorine in CF to inorganic chloride and lowered the yield of DCM. In cultures where no or little CF bioconversion occurred, prolonged exposure to CF resulted in cell lysis, as evidenced by the release of intracellular chloride. The results taken as a whole suggest that the anaerobic bioremediation of CF-contaminated sites can greatly be improved with strategies aimed at increasing the concentration of redox active vitamins.

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Chloroform Cometabolism by Butane-Grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b

Cited by 94 publications

References 32 publications

A Desulfitobacterium sp. strain PR reductively dechlorinates both 1,1,1‐trichloroethane and chloroform

A Desulfitobacterium sp. strain PR reductively dechlorinates both 1,1,1‐trichloroethane and chloroform

Biodefluorination and biotransformation of fluorotelomer alcohols by two alkane‐degrading Pseudomonas strains

Riboflavin‐ and cobalamin‐mediated biodegradation of chloroform in a methanogenic consortium

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