Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells

Atiakshin, Dmitri; Kostin, Andrey; Buchwalow, Igor; Morozow, Denis; Samoilova, Vera; Tiemann, Markus

doi:10.1007/s00418-022-02174-1

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…In particular, the study of the specific MC protease expression, tryptase, is of special significance. Using a combined histochemical approach, we have previously demonstrated increased tryptase expression in a mast cell population under various treatment protocols, primarily molecular hydrogen [ 21 , 22 , 23 ]. Tryptase has a high biological activity, affecting the state of many cellular and non-cellular components of the tissue microenvironment [ 14 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect

et al. 2023

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The mechanisms of regeneration for the fibrous component of the connective tissue of the dermis are still insufficiently studied. The aim of this study was to evaluate the effectiveness of the use of molecular hydrogen on the local therapy of a II degree burn wound with the intensification of collagen fibrillogenesis in the skin. We analyzed the involvement of mast cells (MCs) in the regeneration of the collagen fibers of the connective tissue using water with a high content of molecular hydrogen and in a therapeutic ointment for the cell wounds. Thermal burns led to an increase in the skin MC population, accompanied by a systemic rearrangement of the extracellular matrix. The use of molecular hydrogen for the treatment of burn wounds stimulated the regeneration processes by activating the formation of the fibrous component of the dermis, accelerating wound healing. Thus, the intensification of collagen fibrillogenesis was comparable to the effects of a therapeutic ointment. The remodeling of the extracellular matrix correlated with a decrease in the area of damaged skin. Skin regeneration induced by the activation of the secretory activity of MCs may be one of the possible points of implementation of the biological effects of molecular hydrogen in the treatment of burn wounds. Thus, the positive effects of molecular hydrogen on skin repair can be used in clinical practice to increase the effectiveness of therapy after thermal exposure.

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Section: Discussionmentioning

confidence: 99%

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect

et al. 2023

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“…Enzyme activity of chloroacetate esterase (ChAE) was detected using simultaneous azo‐coupling with naphthol AS‐D chloroacetate as a substrate and with hexazonium‐p‐rosaniline as a coupling agent according to the standard staining protocol (Atiakshin et al, 2023; Leder, 1979; Lojda et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural features of tumor‐associated mast cells in parasympathetic paragangliomas (chemodectomas) of the neck

Chekmaryova,

Kalinin,

Kostin

et al. 2024

Microscopy Res & Technique

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The mechanisms of the pathogenesis of neck paraganglioma (PGL) and the possible role of mast cells (MCs) in its development and metastasis are still poorly understood. We analyzed MCs' morphologic characterization, activation, and the properties of their cytoplasmic/released granules in PGLs, using light and transmission electron microscopy. Paragangliomas showed a large tumor‐associated MC population both in the connective tissue layers of the tumor and between the tumor cells. Notably, MCs were presented by a high expression of specific proteases, size variation, polymorphism, and variable ultrastructural phenotype of granules. A massive number of granules were released surrounding the degranulated MCs while the integrity of MC membrane was maintained. Granules were electron‐dense with or without a membrane, ranging from 0.2 to 0.8 μm in diameter. MC plasmalemma was not found at the site of MC‐collagen fibrils contact, whereas the secretome and fibrils were directly contacted. We observed direct and mediator‐based interactions between MCs and paraganglioma cells. The latter preserved their membrane integrity when MC granules were not in proximity. The effects of the MC secretome on the paraganglioma microenvironment demonstrated its pathogenetic role in tumor progression and allow its application to new diagnostic criteria and the development of protocols for personalized therapy.Research Highlights Ultrastructural analysis reveals novel regulatory effects of mast cells via diverse secretory pathways on the pathogenesis of parasympathetic paraganglioma, including fibrous extracellular matrix remodeling and mediator‐based interactions between MCs and cells of the tumor microenvironment.

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Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells

Cited by 2 publications

References 34 publications

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect

Ultrastructural features of tumor‐associated mast cells in parasympathetic paragangliomas (chemodectomas) of the neck

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