Abstract:Reactive RNA probes are useful for studying and identifying RNA-binding proteins. To that end, we designed and synthesized chloroacetamide-linked 7deaza-ATP which was a good substrate for T7 RNA polymerase in in vitro transcription assay to synthesize reactive RNA probes bearing one or several reactive modifications. Modified RNA probes reacted with thiolcontaining molecules as well as with cysteine-or histidine-containing peptides to form stable covalent products. They also reacted selectively with RNA-bindin… Show more
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