Chlorinated paraffins: Disposition of a highly chlorinated polychlorohexadecane in mice and quail

Abstract

Uniformly 14C-labelled 1-chloro-polychlorohexadecane (PCHD) of high chlorine content (69% w/w) was given to Japanese quail and to C57Bl mice perorally (p.o.) and intravenously (i.v.). The degradation of PCHD to 14CO2, measured during 8 h, was found to be minute (about 1% of dose) in both species after either route of administration. In mice 66 and 43% of dose was eliminated in the feces during 96 h following p.o. and i.v. administration, respectively; the urinary excretion was about 3% in both cases. In quail, the combined fecal and urinary excretion during 96 h after p.o. administration was 58% of dose. The autoradiographic distribution following p.o. administration showed some general similarities between mice and quail; high radioactivities were present in bile, liver, kidney, and intestinal contents up to 24 h after administration. In addition, in quail high radioactivity was present in the hypophysis, retina, blood, and egg yolk. In mice strong accumulation and retention was observed in the corpora lutea up to 30 days after administration. A long time retention in fat occurred in both species.

Risk assessment of chlorinated paraffins in feed and food

“…For the low chlorinated MCCP (Darnerud and Brandt, ), about 33% of the administered radioactivity was exhaled as 14 CO 2 within 12 h after oral administration with 6.5% of the radioactivity in the urine and 14% in the faeces. For the higher chlorinated MCCP (Biessmann et al., ), the elimination of radioactivity in urine was about 3% and about 66% in the faeces during 96 h; the elimination in exhaled air was about 1%.…”

“…In the elimination part of the study with the SCCP and the lower chlorinated MCCP (Biessmann et al., ), the exhalation of 14 CO 2 during 8 hours following dosing with the MCCP was about twice (38.8%) that of the SCCP (21.6%); the excretion of radioactivity in urine and faeces (combined, during 8 hours) was also higher following dosing with the MCCP (13.5%) compared with SCCP (9.8%). For the higher chlorinated MCCP (Biessmann et al., ), the elimination of radioactivity in urine and faeces (combined) was about 58% during 96 hours after administration; the elimination in exhaled air was about 1%.…”

“…The disposition of a highly chlorinated MCCP was investigated in mice (Biessmann et al, 1983). Female C57B1 mice (22 g) were given a single dose of a uniformly 14 C-labelled 1-chloro-polychlorohexadecane (PCHD, C 16 H 20.6 Cl 13.4 , 69% chlorination by weight) orally (4 animals) or by i.v.…”

“…Quantitative differences were observed in the rate of metabolism of the CPs depending on the chain length and/or the degree of chlorination and high chlorination seemed to favour the retention in fatty tissues as the parent compound. The disposition of a highly chlorinated MCCP was investigated in Japanese quail (Biessmann et al, 1983). Female Japanese quail were given a single dose of a uniformly 14 C-labelled 1-chloropolychlorohexadecane (PCHD, C 16 H 20.6 Cl 13.4 , 69% chlorination by weight) orally or by i.v.…”

Studies on Implantation and Embryonic Development in Mice Given a Highly Chlorinated Hexadecane

“…Up to 5000 mg/kg b.wt./day of a long chain, 43% chlorinated CP administered to rats on day 6-19 of gestation gave no increase in the frequency of foetal anomalies. However, in an earlier study we observed a strong accumulation of a highly chlorinated I4C-hexadecane in the ovaries of non-pregnant mice (Biessmann et al 1983). It was therefore of interest to study if a similar chloroalkane (this time unlabelled and in a higher dose) has any effect on the implantation and embryonic development in mice.…”