Chlorinated Hydrocarbon Metabolism

Reineke, Walter; Mandt, Christian; Kaschabek, Stefan R.; Pieper, Dietmar H.

doi:10.1002/9780470015902.a0000472.pub4

Cited by 1 publication

(1 citation statement)

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“…There has been much research into the bacterial metabolism and detection of aromatic hydrocarbons ( Chauhan et al, 2008 ; Plotnikova et al, 2016 ; Reineke et al, 2020 ). Less is known about metabolism and sensing of aliphatic hydrocarbons, in particular the shorter chain gaseous compounds.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review

Moratti

Scott

Coleman

2022

Front. Bioeng. Biotechnol.

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Monooxygenases are a class of enzymes that facilitate the bacterial degradation of alkanes and alkenes. The regulatory components associated with monooxygenases are nature’s own hydrocarbon sensors, and once functionally characterised, these components can be used to create rapid, inexpensive and sensitive biosensors for use in applications such as bioremediation and metabolic engineering. Many bacterial monooxygenases have been identified, yet the regulation of only a few of these have been investigated in detail. A wealth of genetic and functional diversity of regulatory enzymes and promoter elements still remains unexplored and unexploited, both in published genome sequences and in yet-to-be-cultured bacteria. In this review we examine in detail the current state of research on monooxygenase gene regulation, and on the development of transcription-factor-based microbial biosensors for detection of alkanes and alkenes. A new framework for the systematic characterisation of the underlying genetic components and for further development of biosensors is presented, and we identify focus areas that should be targeted to enable progression of more biosensor candidates to commercialisation and deployment in industry and in the environment.

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Section: Introductionmentioning

confidence: 99%