Chloride intracellular channels as novel biomarkers for digestive system tumors (Review)

Wang, Hui; An, Jiaxing; He, Su-yu; Liao, Chengcheng; Wang, Juan; Tuo, Biguang

doi:10.3892/mmr.2021.12269

Cited by 3 publications

(3 citation statements)

References 121 publications

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“…More is known about mitochondrial anion channels belonging to chloride intracellular channel proteins (CLIC) (84, 173,244). There are six homologs in mammals (CLIC1-6), four in plants (DHAR1-4), and two in C. elegans (Exc4 and Exl1).…”

Section: Mitochondrial Inner Mitochondrial Membrane-located Anion-sel...mentioning

confidence: 99%

Mitochondrial Ion Channels

Dębska

Kicińska²,

Skalska³

et al. 2023

Annu. Rev. Biophys.

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Mitochondria are involved in multiple cellular tasks, such as ATP synthesis, metabolism, metabolite and ion transport, regulation of apoptosis, inflammation, signaling, and inheritance of mitochondrial DNA. The majority of the correct functioning of mitochondria is based on the large electrochemical proton gradient, whose component, the inner mitochondrial membrane potential, is strictly controlled by ion transport through mitochondrial membranes. Consequently, mitochondrial function is critically dependent on ion homeostasis, the disturbance of which leads to abnormal cell functions. Therefore, the discovery of mitochondrial ion channels influencing ion permeability through the membrane has defined a new dimension of the function of ion channels in different cell types, mainly linked to the important tasks that mitochondrial ion channels perform in cell life and death. This review summarizes studies on animal mitochondrial ion channels with special focus on their biophysical properties, molecular identity, and regulation. Additionally, the potential of mitochondrial ion channels as therapeutic targets for several diseases is briefly discussed.

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Section: Mitochondrial Inner Mitochondrial Membrane-located Anion-sel...mentioning

confidence: 99%

Mitochondrial Ion Channels

Dębska

Kicińska²,

Skalska³

et al. 2023

Annu. Rev. Biophys.

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“…This may be due to lack of early diagnosis and inconsistent clinical treatment strategies in different regions of China. Its treatment strategies mainly include surgery, radiation therapy, chemotherapy and endoscopic treatment (5). Digestive system tumors exhibit no notable adverse symptoms in the early stages, making early diagnosis difficult.…”

Section: Introductionmentioning

confidence: 99%

Role of SPP1 in the diagnosis of gastrointestinal cancer

Jiang,

Jin,

2023

Oncol Lett

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Recently, the incidence rate of digestive system tumors has increased in China and these tumors occur in a younger population. The present study aimed to determine the expression levels and potential clinical value of secreted phosphoprotein 1 (SPP1) in gastrointestinal cancer. The microarray datasets GSE104836, GSE189830 and GSE103236, obtained from the gene expression omnibus database, were analyzed to determine differentially expressed genes in patients with colorectal cancer (CRC), gastric cancer (GC) and esophageal cancer (EC). A total of 42 patients with CRC, GC or EC and 21 healthy controls were recruited to obtain blood and tissues samples. SPP1 expression levels were detected using reverse transcription-quantitative PCR. Moreover, levels of significance of SPP1 in patients with CRC, GC and EC were analyzed using receiver operating characteristic analysis. Potential correlations between SPP1 and carcinoembryonic antigen (CEA) were assessed using Pearson's correlation coefficient. SPP1 was significantly upregulated in the serum, plasma and tissue of patients with CRC, GC or EC. In addition, the area under the curve of SPP1 was >0.5 in the plasma, serum and cancer tissue of patients with early and late CRC, GC or EC. The present study further demonstrated that the specificity and sensitivity of SPP1 was higher in patients with late CRC, GC or EC compared with patients with early CRC, GC or EC. Moreover, SPP1 and CEA were significantly positively correlated in serum of patients with CRC, GC or EC. In conclusion, the current study demonstrated that SPP1 exhibited significant diagnostic value for gastrointestinal tumors, which suggested that SPP1 may exhibit potential as a diagnostic marker of CRC, GC and EC. The present study provided a novel theoretical basis for the role of SPP1 as a diagnostic marker of digestive system tumors.

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“…More recently, chloride intracellular channel (CLIC), a class of intracellular organelle ion channels, has attracted great attention in many species ( Ashley, 2003 ). For mammals, the CLIC superfamily (CLIC1-6) mainly regulates a variety of cellular events, including cell differentiation, apoptosis and metastatic competence ( Argenzio and Moolenaar, 2016 ; Wang et al, 2021 ). Growing evidence also supports the role of mammalian CLIC1-6 in different diseases ( Leanza et al, 2013 ), especially CLIC1 ( Francisco et al, 2020 ) and CLIC4 ( Chuang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Chloride intracellular channel gene knockdown induces insect cell lines death and level increases of intracellular calcium ions

Song

Yan

et al. 2023

Front. Physiol.

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Chloride intracellular channel (CLIC) is a member of the chloride channel protein family for which growing evidence supports a pivotal role in fundamental cellular events. However, the physiological function of CLIC in insects is still rarely uncovered. The ovary-derived High Five (Hi-5) cell line isolated from the cabbage looper (Trichoplusia ni) is widely used in laboratories. Here, we studied both characteristics and functions of CLIC in Hi-5 cells (TnCLIC). We identified the TnCLIC gene in Hi-5 cells and annotated highly conserved CLIC proteins in most insect species. After RNA interference of TnCLIC, the phenomenon of significantly increased cell death suggests that the TnCLIC protein is essential for the survival of Hi-5 cells. The same lethal effect was also observed in Spodoptera frugiperda 9 and Drosophila melanogaster Schneider 2 cells after CLIC knockdown. Furthermore, we found that this kind of cell death was accompanied by increases in intracellular calcium ions after TnCLIC knockdown with the transcriptomic analyses and the detection of calcium levels. Our results provide insights into insect CLIC as a key factor for cell survival and lay the foundation for the cell death mechanism.

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Chloride intracellular channels as novel biomarkers for digestive system tumors (Review)

Cited by 3 publications

References 121 publications

Mitochondrial Ion Channels

Mitochondrial Ion Channels

Role of SPP1 in the diagnosis of gastrointestinal cancer

Chloride intracellular channel gene knockdown induces insect cell lines death and level increases of intracellular calcium ions

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