Chloride intracellular channel protein 2 is secreted and inhibits MMP14 activity, while preventing tumor cell invasion and metastasis

Ozaki, Saya; Akihiro, Umakoshi; Yano, Hajime; Ohsumi, S; Sumida, Yutaro; Hayase, Erika; Usa, Eika; Islam, Afsana; Choudhury, Mohammed E.; Nishi, Yusuke; Yamashita, Daisuke; Ohtsuka, Yoshihiro; Nishikawa, Masahiro; Inoue, Akihiro; Suehiro, Satoshi; Kuwabara, Jun; Watanabe, Hideaki; Takada, Yasutsugu; Watanabe, Yuji; Nakano, ﻿Ichiro; Kunieda, Takeharu; Tanaka, Junya

doi:10.1016/j.neo.2021.06.001

Cited by 13 publications

(9 citation statements)

References 45 publications

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“…Of those, GJA1, AQP1, SCN4B and AQP7 were stated previously in breast cancer tumor growth [41][42][43][44]. Similarly, PKD2, GJA4, KCNJ8, TRPC1, KCNJ2, KCND2, KCNB1, CLIC5, CLIC2 and GABRE were previously reported to have a role in tumor growth in several tumors [45][46][47][48][49][50][51][52][53][54][55][56]. HTR3C, CLCN6, GLRB, SCN3A, ANO3, ANO5, ANO6 and LRRC8C were identified in this study as putative ion channels that may be implicated in tumor growth and development in breast cancer.…”

Section: Discussionmentioning

confidence: 86%

In Silico Analysis of Ion Channels and Their Correlation with Epithelial to Mesenchymal Transition in Breast Cancer

Parthasarathi

Mandal

Singh

et al. 2022

Cancers

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Uncontrolled growth of breast cells due to altered gene expression is a key feature of breast cancer. Alterations in the expression of ion channels lead to variations in cellular activities, thus contributing to attributes of cancer hallmarks. Changes in the expression levels of ion channels were observed as a consequence of EMT. Additionally, ion channels were reported in the activation of EMT and maintenance of a mesenchymal phenotype. Here, to identify altered ion channels in breast cancer patients, differential gene expression and weighted gene co-expression network analyses were performed using transcriptomic data. Protein–protein interactions network analysis was carried out to determine the ion channels interacting with hub EMT-related genes in breast cancer. Thirty-two ion channels were found interacting with twenty-six hub EMT-related genes. The identified ion channels were further correlated with EMT scores, indicating mesenchymal phenotype. Further, the pathway map was generated to represent a snapshot of deregulated cellular processes by altered ion channels and EMT-related genes. Kaplan–Meier five-year survival analysis and Cox regressions indicated the expression of CACNA1B, ANO6, TRPV3, VDAC1 and VDAC2 to be potentially associated with poor survival. Deregulated ion channels correlate with EMT-related genes and have a crucial role in breast cancer-associated tumorigenesis. Most likely, they are potential candidates for the determination of prognosis in patients with breast cancer.

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Section: Discussionmentioning

confidence: 86%

In Silico Analysis of Ion Channels and Their Correlation with Epithelial to Mesenchymal Transition in Breast Cancer

Parthasarathi

Mandal

Singh

et al. 2022

Cancers

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“…An RNA library was prepared from PrL tissues dissected from three Wistar rats and three LHRs reared in the standard condition as well as from three LHRs reared in the EE condition using a TruSeq RNA Sample Prep Kit v2-Set A (Illumina, CA, USA) according to the manufacturer’s instructions. The tissues were subjected to RNAseq on a MiSeq NGS sequencer (Illumina) as described elsewhere [ 36 ]. Genes with significant differential expression (uncorrected p , <0.05) and log 2 (fold change) >|0.3875| were selected and analyzed using the free software R [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Rearing in an Enriched Environment Ameliorates the ADHD-like Behaviors of Lister Hooded Rats While Suppressing Neuronal Activities in the Medial Prefrontal Cortex

Utsunomiya

Mikami

Doi

et al. 2022

Cells

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In addition to genetic factors, environmental factors play a role in the pathogenesis of attention deficit/hyperactivity disorder (ADHD). This study used Lister hooded rats (LHRs) as ADHD model animals to evaluate the effects of environmental factors. Male LHR pups were kept in four rearing conditions from postnatal day 23 (4 rats in a standard cage; 12 rats in a large flat cage; and 4 or 12 rats in an enriched environment [EE]) until 9 weeks of age. EE rearing but not rearing in a large flat cage decreased the activity of LHRs in the open field test that was conducted for 7 consecutive days. In the drop test, most rats reared in an EE remained on a disk at a height, whereas most rats reared in a standard cage fell off. RNA sequencing revealed that the immediate-early gene expression in the medial prefrontal cortex of LHRs reared in an EE was reduced. cFos-expressing neurons were reduced in number in LHRs reared in an EE. These results suggest that growing in an EE improves ADHD-like behaviors and that said improvement is due to the suppression of neuronal activity in the mPFC.

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“…The low magnification image is shown in Supplementary Figure 1 for pointing the area of the brain from which the immunofluorescent image was derived. Images of positive control (tumor tissue) and negative controls (healthy tissue), no primary antibody controls for Bcl-xL are shown in Supplementary Figure 2 (Karmakar et al, 2007;Ozaki et al, 2021).…”

Section: Immunofluorescencementioning

confidence: 99%

Microglial re-modeling contributes to recovery from ischemic injury of rat brain: A study using a cytokine mixture containing granulocyte-macrophage colony-stimulating factor and interleukin-3

et al. 2022

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Ischemic stroke is a leading cause of mortality and permanent disability. Chronic stroke lesions increase gradually due to the secondary neuroinflammation that occurs following acute ischemic neuronal degeneration. In this study, the ameliorating effect of a cytokine mixture consisting of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-3 was evaluated on ischemic brain injury using a rat stroke model prepared by transient middle cerebral artery occlusion (tMCAO). The mixture reduced infarct volume and ameliorated ischemia-induced motor and cognitive dysfunctions. Sorted microglia cells from the ischemic hemisphere of rats administered the mixture showed reduced mRNA expression of tumor necrosis factor (TNF)-α and IL-1β at 3 days post-reperfusion. On flow cytometric analysis, the expression of CD86, a marker of pro-inflammatory type microglia, was suppressed, and the expression of CD163, a marker of tissue-repairing type microglia, was increased by the cytokine treatment. Immunoblotting and immunohistochemistry data showed that the cytokines increased the expression of the anti-apoptotic protein Bcl-xL in neurons in the ischemic lesion. Thus, the present study demonstrated that cytokine treatment markedly suppressed neurodegeneration during the chronic phase in the rat stroke model. The neuroprotective effects may be mediated by phenotypic changes of microglia that presumably lead to increased expression of Bcl-xL in ischemic lesions, while enhancing neuronal survival.

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Chloride intracellular channel protein 2 is secreted and inhibits MMP14 activity, while preventing tumor cell invasion and metastasis

Cited by 13 publications

References 45 publications

In Silico Analysis of Ion Channels and Their Correlation with Epithelial to Mesenchymal Transition in Breast Cancer

In Silico Analysis of Ion Channels and Their Correlation with Epithelial to Mesenchymal Transition in Breast Cancer

Rearing in an Enriched Environment Ameliorates the ADHD-like Behaviors of Lister Hooded Rats While Suppressing Neuronal Activities in the Medial Prefrontal Cortex

Microglial re-modeling contributes to recovery from ischemic injury of rat brain: A study using a cytokine mixture containing granulocyte-macrophage colony-stimulating factor and interleukin-3

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