Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling

Geng, Huiwu; Cao, Feng; Sun, Zhangran; Xu, Fan; Xie, Yingping; Gu, Junjie; Фан, Ли; Liu, Gang; Li, Chao; Thorne, Rick F.; Zhang, Xu Dong; Li, Xinying; Li, Xiaoying

doi:10.1016/j.tranon.2022.101560

Cited by 9 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In esophageal squamous cell carcinoma (ESSC), Geng et al found significant overexpression of CLIC1 on the level of mRNA and protein in the cancer tissues in comparison with normal adjacent tissues and correlation of CLIC1 expression with the TNM classification [19].…”

Section: Discussionmentioning

confidence: 99%

Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Wojtera,

Ostrowska,

Szewczyk

et al. 2024

Rep Pract Oncol Radiother.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Wojtera,

Ostrowska,

Szewczyk

et al. 2024

Rep Pract Oncol Radiother.

View full text Add to dashboard Cite

show abstract

“…In breast cancer, elevated CLIC1 expression correlates with tumor characteristics like size, TNM classification, grade, lymph node metastasis, and Ki67, while lower expression associates with extended OS and progression-free survival, suggesting its diagnostic potential ( Xia et al, 2022 ). In esophageal squamous cell carcinoma (ESCC), CLIC1 is linked to clinical TNM classifications ( Geng et al, 2022 ). CLIC1 knockdown in ESCC cell lines inhibits mTOR signaling, affecting cell proliferation and protein expression ( Geng et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…In esophageal squamous cell carcinoma (ESCC), CLIC1 is linked to clinical TNM classifications ( Geng et al, 2022 ). CLIC1 knockdown in ESCC cell lines inhibits mTOR signaling, affecting cell proliferation and protein expression ( Geng et al, 2022 ). High CLIC1 expression in lung adenocarcinoma predicts shorter overall survival and functions as an independent prognostic factor ( Yasuda et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Integrating bulk and single-cell RNA sequencing data reveals epithelial-mesenchymal transition molecular subtype and signature to predict prognosis, immunotherapy efficacy, and drug candidates in low-grade gliomas

Wang,

2023

Front. Pharmacol.

View full text Add to dashboard Cite

Objective: Epithelial-mesenchymal transition (EMT) is a tightly regulated and dynamic process occurring in both embryonic development and tumor progression. Our study aimed to comprehensively explore the molecular subtypes, immune landscape, and prognostic signature based on EMT-related genes in low-grade gliomas (LGG) in order to facilitate treatment decision-making and drug discovery.Methods: We curated EMT-related genes and performed molecular subtyping with consensus clustering algorithm to determine EMT expression patterns in LGG. The infiltration level of diverse immune cell subsets was evaluated by implementing the single-sample gene set enrichment analysis (ssGSEA) and ESTIMATE algorithms. The distinctions in clinical characteristics, mutation landscape, and immune tumor microenvironment (TME) among the subtypes were subjected to further investigation. Gene Set Variation Analysis (GSVA) was performed to explore the biological pathways that were involved in subtypes. The chemo drug sensitivity and immunotherapy of subtypes were estimated through GDSC database and NTP algorithm. To detect EMT subtype-related prognostic gene modules, the analysis of weighted gene co-expression network (WGCNA) was performed. The LASSO algorithm was utilized to construct a prognostic risk model, and its efficacy was verified through an independent CGGA dataset. Finally, the expression of the hub genes from the prognostic model was evaluated through the single-cell dataset and in-vitro experiment.Results: The TCGA-LGG dataset revealed the creation of two molecular subtypes that presented different prognoses, clinical implications, TME, mutation landscapes, chemotherapy, and immunotherapy. A three-gene signature (SLC39A1, CTSA and CLIC1) based on EMT expression pattern were established through WGCNA analysis. Low-risk patients showed a positive outlook, increased immune cell presence, and higher expression of immune checkpoint proteins. In addition, several promising drugs, including birinapant, fluvastatin, clofarabine, dasatinib, tanespimycin, TAK−733, GDC−0152, AZD8330, trametinib and ingenol-mebutate had great potential to the treatment of high risk patients. Finally, CTSA and CLIC1 were highly expressed in monocyte cell through single-cell RNA sequencing analysis.Conclusion: Our research revealed non-negligible role of EMT in the TME diversity and complexity of LGG. A prognostic signature may contribute to the personalized treatment and prognostic determination.

show abstract

“…Traditional treatments for EC, such as surgery, radiation therapy, and chemotherapy, have a signi cant impact on patients' quality of life and are associated with low effectiveness rates, 29 warranting, new effective therapeutic approaches. Immune checkpoint inhibition and CAR T-cell therapy have improved cancer immunotherapy, 30 providing patients with hope for a possible cure.…”

Section: Discussionmentioning

confidence: 99%

Elucidating the Correlation between Leupaxin as a Prognostic Biomarker and Immunotherapeutic Efficacy in Esophageal Squamous Cell Carcinoma

Teng,

Chen,

Liao

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Oesophageal cancer is a prevalent malignant tumour globally, yet it often receives subpar treatment. Recent interest in immunotherapy has instilled hope for cancer patients. However, further research is necessary to comprehend the mechanisms and roles of effective biomarker in oesophageal squamous carcinoma (ESCC). Methods: We investigated the expression, pathological and prognostic significance, protein interactions, pathway enrichment, immune microenvironment, correlations between immune regulators and infiltration of immune cells, associations with drug resistance genes, as well as chemosensitivity of leupaxin (LPXN) in ESCC. The relative expression of LPXN mRNA and protein was evaluated in both healthy surrounding tissues and ESCC tissues using quantitative polymerase chain reaction and immunohistochemistry, utilizing samples obtained from clinical cases. Furthermore, we investigated the potential role of LPXN in ESCC through a variety of techniques including cell proliferation assays, apoptosis assays, clonogenic assays, migration assays with or without extracellular matrix support. The co-expression of LPXN and PD-L1 at the protein level in oesophageal squamous cell lines was determined by western blotting. We validated the expression of the LPXN gene in ESCC using clinical samples and investigated the correlation between LPXN gene expression and the efficacy of immune therapy for ESCC. Results:Bioinformatic analysis revealed a favourable association between LPXN and prognosis in ESCC. LPXN and PD-L1 were found to be co-expressed, with LPXN strongly associated with the immune system, related pathways, microenvironment, regulators, cell infiltration levels, genes related to drug resistance, and chemosensitivity. The expression of LPXN in ESCC at both mRNA and protein levels was confirmed in clinical samples of cancerous and normal tissues. Cell-based experiments demonstrated that knockdown of LPXN resulted in decreased cell viability, invasion, proliferation, and migration in ESCC cell lines. In samples that responded well to therapy, LPXN and PD-L1 were overexpressed at the mRNA and protein levels. Conclusion: The results of our study have revealed the significance and involvement of the immune-related biomarker LPXN in the proliferation and migration processes of ESCC, thereby establishing a novel framework for the treatment of this disease.

show abstract

Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling

Cited by 9 publications

References 48 publications

Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Integrating bulk and single-cell RNA sequencing data reveals epithelial-mesenchymal transition molecular subtype and signature to predict prognosis, immunotherapy efficacy, and drug candidates in low-grade gliomas

Elucidating the Correlation between Leupaxin as a Prognostic Biomarker and Immunotherapeutic Efficacy in Esophageal Squamous Cell Carcinoma

Contact Info

Product

Resources

About