Chloride currents in cones modify feedback from horizontal cells to cones in goldfish retina

Abstract

Key points• The GABAergic pathway modulates feedback between retinal horizontal cells (HCs) and cone photoreceptors, but is not mediating negative feedback, as previously hypothesized.• Opening of GABA-gated chloride channels in cone photoreceptors reduces the amplitude of feedback responses generated by HCs.• Activation of a different presynaptic chloride current, the calcium-dependent chloride current, in individual cones has a similar effect on feedback as application of GABA.• Modulation of the strength of feedback from HCs seems to be a general consequence of activation of presynaptic chloride currents in cones.• This puts the functional role of these currents in a new perspective; GABA acts as a slow and global neuromodulator enhancing feedback in the light-and attenuating feedback in the dark-adapted retina, whereas the calcium-dependent chloride current modulates feedback fast and locally to tune the size of feedback to local light conditions. AbstractIn neuronal systems, excitation and inhibition must be well balanced to ensure reliable information transfer. The cone/horizontal cell (HC) interaction in the retina is an example of this. Because natural scenes encompass an enormous intensity range both in temporal and spatial domains, the balance between excitation and inhibition in the outer retina needs to be adaptable. How this is achieved is unknown. Using electrophysiological techniques in the isolated retina of the goldfish, it was found that opening Ca 2+ -dependent Cl − channels in recorded cones reduced the size of feedback responses measured in both cones and HCs. Furthermore, we show that cones express Cl − channels that are gated by GABA released from HCs. Similar to activation of I Cl(Ca) , opening of these GABA-gated Cl − channels reduced the size of light-induced feedback responses both in cones and HCs. Conversely, application of picrotoxin, a blocker of GABA A and GABA C receptors, had the opposite effect. In addition, reducing GABA release from HCs by blocking GABA transporters also led to an increase in the size of feedback. Because the independent manipulation of Ca 2+ -dependent Cl − currents in individual cones yielded results comparable to bath-applied GABA, it was concluded that activation of either Cl − current by itself is sufficient to reduce the size of HC feedback. However, additional effects of GABA on outer retinal processing cannot be excluded. These results can be accounted for by an ephaptic feedback model in which a cone Cl − current shunts the current flow in the synaptic cleft. The Ca 2+ -dependent Cl − current might be essential to set the initial balance between the feedforward and the feedback signals active in the cone HC synapse. It prevents that strong feedback from HCs to cones flood the cone with Ca 2+ . Modulation of the feedback strength by GABA might play a role during light/dark adaptation, adjusting the amount of negative feedback to the signal to noise ratio of the cone output.

Cellular and Circuit Mechanisms Shaping the Perceptual Properties of the Primate Fovea

“…Voltage responses of foveal and peripheral cones changed little following suppression of horizontal cell activity (Figures 7E and 7F). This is consistent with work indicating that horizontal cell feedback modulates a conductance with a reversal potential close to the cone photoreceptor resting potential, and hence impacts transmitter release more noticeably than the cone light response (Endeman et al, 2012; Verweij et al, 2003). To test for a role of gap junctions, we performed voltage clamp recordings from foveal cones after omitting ATP and GTP from the intracellular solution.…”

“…The differences in kinetics of light-dependent voltage changes of foveal and peripheral cones could originate within the phototransduction process, from electrical properties of the inner segment, or from network interactions mediated by horizontal cells or gap-junctional coupling (Barrow and Wu, 2009; Endeman et al, 2012; Hornstein et al, 2005a; Hornstein et al, 2005b; Verweij et al, 2003). To test for contributions of inner segment conductances, we measured cone currents using voltage-clamp recordings.…”

Invaginating Presynaptic Terminals in Neuromuscular Junctions, Photoreceptor Terminals, and Other Synapses of Animals

“…And as noted, Hirano et al (2016) show evidence supporting a GABA mechanism. However, this latter mechanism seems to be indirect, with GABA released from horizontal cells and activating autoreceptors on the horizontal cells, possibly driving a pH-based feedback mechanism (Liu et al 2013; Hirano et al 2016); a GABA autoreceptor-based mechanism also might be present in lower vertebrates (Klooster et al 2004; Endeman et al 2012). …”

Feedback‐induced glutamate spillover enhances negative feedback from horizontal cells to cones

“…), GABA modulates the strength of this pathway (Endeman et al . ). Furthermore, HCs also express GABA A ‐receptors.…”

“…; Endeman et al . ). It seems that the surround‐induced depolarizing responses in cones are caused by the activation of I Cl(Ca) (O'Bryan, ; Lasansky, ; Piccolino et al .…”