Chloride channel 2 gene (Clc2) maps to chromosome 16 of the mouse, extending a region of conserved synteny with human chromosome 3q

Abstract

The Clc2 gene of the mouse codes for the ubiquitously expressed chloride channel ClC-2, a member of a family of at least seven voltage gated chloride channels, some of which are implicated in hereditary diseases. Using a mouse interspecies back-cross panel, we have mapped Clc2 to Chr 16, proximal to the somatostatin gene Smst, extending a region of documented conserved synteny to human Chr 3q.

Cloning and chromosomal mapping of the human p53-related KET gene to Chromosome 3q27 and its murine homolog Ket to mouse Chromosome 16

“…1) as follows: hKET9, amino acid residues 360-390; muKET8, amino acid residues 360-400; muKET9, amino acid residues 383-438. PCRs were performed as described elsewhere (Lengeling et al 1995). Nucleotide sequences of primers for MIT microsatellite marker D16Mit57 were obtained from the MIT mouse genome database.…”

“…To reveal wether the murine Ket locus falls into the homologous region, mapping of the Ket gene was performed with an interspecific mouse backcross (C57BL/6J wrl+ × SEG/1 +/+)*F 1 wrl+ × (C57BL/6J wrl+) originally established to map the wobbler gene (Kaupmann et al 1992). This interspecific backcross panel has been typed for over 150 loci distributed over all autosomes and the X Chr; a refined map of Chr 16 has been created for the mapping of the chloride channel gene Clc2 (Lengeling et al 1995). Both murine KET PCR fragments provided informative restriction fragment length variants (RFLVs) that were used for segregation analysis.…”