Chloride analysis of botanical samples by ICP-OES

Abstract

A unique method for chloride analysis of botanical samples by axially viewed ICP-OES with a modified optical system is described. Calibration on the most sensitive wavelength (134.7 nm) between 1 and 200 mg Cl L À1 yielded a curvilinear relationship (R 2 ¼ 1.000) across three orders of magnitude. The instrumental limits of detection in solution of 0.041 mg L À1 and background equivalent concentration of 5.29 mg L À1 are similar to those of other methods using pneumatic nebulisers. Matrix matching of calibration standards with respect to nitric acid concentration (4% v/v) and carbon levels (0.15% sucrose m/v) of extracted sample solutions produced consistent responses from plant material. High carbon concentrations (up to 8% sucrose m/v) caused matrix interferences that decreased blank values by 4.5 mg L À1 and suppressed Cl signals by 20%. No interferences from countercations were observed. A simple extraction process for dried plant samples (0.1 g in 40 mL of 4% HNO 3 ) was developed and tested with plant standard reference materials. Recovery of Cl from the majority of materials was close to 100% of established values while relative standard deviations were below 4% except when chloride concentration was close to the method detection limit. The extraction method was robust to modifications for samples with low mass or high Cl concentrations.

Towards the understanding of chloride profiles in marine exposed concrete, impact of leaching and moisture content

“…This was because the halogens are not easy to excite in a plasma source and their emission wavelengths are difficult to detect. However, recent hardware improvements allow for the determination of chlorine by ICP [19]. In Fig.…”

Quantitative Trait Loci for Chloride Tolerance in ‘Osage’ Soybean

“…Subsequently, the leaf samples were ground to fine powder using a coffee bean grinder (Krups). The fine powder sample was used for Cl − extraction and quantified by inductively coupled plasma optical emission spectroscopy (ICP-OES), as described by Wheal and Lyndon (2010). The Cl − concentration was converted into mg kg −1 .…”

Grapevine and Arabidopsis cation-chloride cotransporters localise to the Golgi and trans-Golgi network and indirectly influence long-distance ion homeostasis and plant salt tolerance

“…Cation concentration was measured by acid extraction and radial-view ICP-OES (Wheal et al, 2011). Chloride concentration was measured by axial-view ICP-OES of 4% (v/v) nitric acid extracts as described by Wheal and Palmer (2010).…”