Chlorella vulgaris phycoremediation at low Cu+2 contents: Proteomic profiling of microalgal metabolism related to fatty acids and CO2 fixation

Andrade, Lidiane Maria de; Tito, Caique Alves; Mascarenhas, Camila; Lima, Fabíola Aliaga de; Dias, Mirella; Andrade, Cristiano José de; Mendes, Maria Anita; Nascimento, Cláudio Augusto Oller do

doi:10.1016/j.chemosphere.2021.131272

Cited by 13 publications

(3 citation statements)

References 33 publications

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Section: Tryptic Digestion For Mass Spectrometry Analysismentioning

confidence: 99%

“…Trypsin solution was prepared using the bovine pancreas trypsin enzyme (Sigma Aldrich, St. Louis, MI, USA), which was solubilized in 400 µL of 50 mM ammonium bicarbonate solution (NH 4 HCO 3 ) to reach a final concentration of 0.05 ng of enzyme/nL of solution [29]. The tryptic digestion was performed using 0.2 mg of lyophilized samples (freeze-dryer model Freezone 4.5, LabConco, Kansas City, MI, USA), adding 50 µL trypsin solution (0.05 ng/nL), and then incubating at 37 • C for 18 h [29]. After this period, the digestion process was interrupted by the addition of 10 µL of 10% (v/v) trifluoroacetic acid, and the solution was kept at 37 • C for another 90 min, followed by centrifugation for 30 min at 6 • C, 17,400 g (CT15RE, Himac, Hitachinaka, Ibaraki, Japan), and then the supernatant containing the digested proteins was transferred to vials containing inserts for subsequent analysis.…”

Section: Tryptic Digestion For Mass Spectrometry Analysismentioning

confidence: 99%

“…In this sense, Ni and Cd at ≥2 mg/L affect microalgal growth [23][24][25]. The Cu is also toxic at high concentrations since it induces microalgal oxidative stress affecting algal photoprotection mechanisms and photosynthesis, pigment synthesis respiration processes, fatty acid production, and CO 2 fixation processes, and it also alters protein production [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

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Phycoremediation of Copper by Chlorella protothecoides (UTEX 256): Proteomics of Protein Biosynthesis and Stress Response

Andrade

Tito

Mascarenhas

et al. 2022

Biomass

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Phycoremediation is an eco-friendly treatment for mining wastes. Copper at high concentrations is toxic for microalgae growth (bioremediation). Proteomics is a modern approach that can assist in elucidating, in detail, the highly complex metabolic mechanisms related to phycoremediation. Therefore, this study aimed to evaluate the effect of copper ions (Cu2+) on the metabolism of Chlorella protothecoides (UTEX 256), particularly the proteome changes. The WC culture medium supplemented with Cu2+ at 0.3, 0.6, and 0.9 mg/L showed a strict correlation to Cu2+ removal of 40, 33, and 36% of the initial content, respectively. In addition, Cu2+ concentrations did not affect microalgae growth—a very traditional approach to measuring toxicity. However, the proteomics data indicated that when compared to the control, reductions in protein levels were observed, and the 10 most scored proteins were related to the light-harvesting complex. Interestingly, C. protothecoides cultivated at 0.9 mg of Cu2+/L biosynthesized the protein Ycf3-interacting chloroplastic isoform X1 to respond to the photooxidative stress and the DNA-directed RNA polymerase III subunit RPC5 was related to the Cu2+ binding. Pre-mRNA-processing factor 19 and cytochrome c peroxidase proteins were observed only in the copper-containing treatments indicating the activation of antioxidant mechanisms by reactive oxygen species, which are potential environmental pollutant biomarkers.

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Section: Tryptic Digestion For Mass Spectrometry Analysismentioning

confidence: 99%

Section: Tryptic Digestion For Mass Spectrometry Analysismentioning

confidence: 99%