Chlorella vulgaris cultivation in sludge extracts from 2,4,6-TCP wastewater treatment for toxicity removal and utilization

Wang, Lu; Chen, Xiurong; Wang, Hualin; Zhang, Yuying; Tang, Qingli; Li, Jiahui

doi:10.1016/j.jenvman.2016.11.020

Cited by 44 publications

(15 citation statements)

References 39 publications

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“…As discussed in 'The main source of sludge toxicity' section, the organic biotoxicity resulting from the degradation of resorcinol was strongly associated with mass fractions of TOC at 5000-10 000 Da. Wang et al discovered that the removal of sludge toxicity was correlated with the removal of TOC, 17 but there was no clear conclusion regarding the main contributor to this toxicity. Therefore, the TOC at different molecular weight ranges was investigated during biotoxicity removal in the cultures of C. vulgaris, and the results are shown in Figs 5 and 6.…”

Section: Toc and Toxicity Removal By C Vulgarismentioning

confidence: 99%

“…16 Therefore, the effects of the above toxicity on the growth of microalgae should be considered when considering sludge extract from industrial wastewater. 10,17 Chlorella vulgaris has been widely studied for its rapid growth rate, efficient utilization of solar energy 18,19 and strong adaptability to polluted environments (e.g. heavy metals, 20 pesticides, 21 redox stress, 22 salt stress 23 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, C. vulgaris has been applied to wastewater because of its capacity to effectively remove soluble organic contaminants, and some refractory macromolecules . According to a previous study, by our group, C. vulgaris grew well in sludge extract from 2,4,6‐ trichlorophenol wastewater diluted with BG11, and showed efficient removal of sludge toxicity arising from the decomposition of TOC (Total organic carbon) . However, there was no clear mechanism of inhibition of sludge organic biotoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Cultivation of Chlorella vulgaris in sludge extract from resorcinol‐rich wastewater: the removal and inhibitory effect of sludge toxicity

Chen

Zhou

Wang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The cultivation of Chlorella vulgaris present in toxic sludge extract from resorcinol‐rich wastewater was investigated to simultaneously determine the biomass production and toxicity removal. RESULTS A comparison of the blank and the toxic sludge extract revealed that the sludge toxicity originating from the degradation of resorcinol was highly correlated to the mass fraction at 5000–10000 Da (R = 0.987, P = 0.013) and led to a decline in biomass (1.49 g L−1 to 1.36 g L−1 in 30 days) along with a reduced utilization rate of organics in the sludge extract, especially in the early stage (0–5 days) of culture. The effects of sludge toxicity were characterized by the downregulation of proteins associated with carbon (C) metabolism and the upregulation of proteins involved in photoreaction by proteomics. CONCLUSION The sludge toxicity directly caused a decline in biomass production of C. vulgaris resulting from the downregulation of C metabolism. However, the promotion of biomass for heterotrophism was more significant than the inhibitory effect in the toxic sludge extract when compared with BG11 medium. © 2018 Society of Chemical Industry

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Section: Toc and Toxicity Removal By C Vulgarismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultivation of Chlorella vulgaris in sludge extract from resorcinol‐rich wastewater: the removal and inhibitory effect of sludge toxicity

Chen

Zhou

Wang

et al. 2019

J of Chemical Tech & Biotech

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“…A Chlorella vulgaris é uma alga verde unicelular encontrada na água doce, que tem sido utilizada em estudos recentes para analisar o impacto de determinados poluentes na vida aquática. Wang et al (2017) cultivou esta espécie em extratos de lodo de tratamento de efluentes para remoção da toxicidade da água. Como resultado do estudo, a Chlorella vulgaris mostrou-se capaz de remover 45% do nitrogênio total e 90% do fósforo total.…”

Section: Chlorella Vulgarisunclassified

Algas como bioindicadores da qualidade da água

Marques¹,

Américo-Pinheiro²

2017

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“…It can grow phototrophically, heterotrophically, or mixotrophically to high biomass, producing different amounts of proteins and lipids 1 . It grows rapidly, dividing into four cells in about 6 h. Because Chlorella synthesizes large amounts of proteins and lipids, it has been used as a raw material for biodiesel and also as a food additive 2 – 4 . Chlorella undergoes posttranslational modifications such as protein N-glycosylation 5 .…”

Section: Introductionmentioning

confidence: 99%

The establishment of new protein expression system using N starvation inducible promoters in Chlorella

Shin

Choi

Jeon

et al. 2020

Sci Rep

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Chlorella is a unicellular green microalga that has been used in fields such as bioenergy production and food supplementation. In this study, two promoters of N (nitrogen) deficiency-inducible Chlorella vulgaris N Deficiency Inducible (CvNDI) genes were isolated from Chlorella vulgaris UTEX 395. These promoters were used for the production of a recombinant protein, human granulocyte-colony stimulating factor (hG-cSf) in Chlorella vulgaris UTEX 395 and Chlorella sp. ArM0029B. To efficiently secrete the hG-cSf, the protein expression vectors incorporated novel signal peptides obtained from a secretomics analysis of Chlorella spp. After a stable transformation of those vectors with a codonoptimized hG-CSF sequence, hG-cSf polypeptides were successfully produced in the spent media of the transgenic Chlorella. To our knowledge, this is the first report of recombinant protein expression using endogenous gene components of Chlorella. Chlorella is a single-celled eukaryote belonging to the green algae. It can grow phototrophically, heterotrophically, or mixotrophically to high biomass, producing different amounts of proteins and lipids 1. It grows rapidly, dividing into four cells in about 6 h. Because Chlorella synthesizes large amounts of proteins and lipids, it has been used as a raw material for biodiesel and also as a food additive 2-4. Chlorella undergoes posttranslational modifications such as protein N-glycosylation 5. The use of Chlorella for the production of valuable proteins has therefore been attempted for more than two decades 6-9. Chen et al. 10 showed expression of rabbit neutrophil peptide using a translational enhancer omega in Chlorella ellipsoidea. Kim et al. 11 produced flounder growth hormone using a 35S cauliflower mosaic virus (35S) promoter in C. ellipsoidea and showed a 25% growth increase of flounder fry. No recombinant proteins produced from Chlorella species have yet been commercialized, although the species has been studied intensively for the potential production of biodiesel. An optimized expression system for producing proteins in Chlorella is urgently needed. Recombinant protein production studies require a highly efficient method for the transformation of Chlorella. Various transformation techniques, including electroporation, PEG transformation, particle bombardment, and Agrobacterium cocultivation, have been used for the efficient transformation of Chlorella spp. 10-14. Recently, Kumar et al. 15 reported an effective method for enhancing transformation efficiency by more than 100-fold using electroporation combined with efficient protoplasting of Chlorella. Besides efficient transformation, an optimized system for the endogenous expression of proteins is indispensable. An expression system involving appropriate promoters is one of the most important factors for highefficiency production of recombinant proteins. In Chlamydomonas reinhardtii, the promoter of the heat shock protein gene HSP70a has been widely used for the expression of foreign genes in C. reinhardtii 16,17. In recent yea...

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Chlorella vulgaris cultivation in sludge extracts from 2,4,6-TCP wastewater treatment for toxicity removal and utilization

Cited by 44 publications

References 39 publications

Cultivation of Chlorella vulgaris in sludge extract from resorcinol‐rich wastewater: the removal and inhibitory effect of sludge toxicity

Cultivation of Chlorella vulgaris in sludge extract from resorcinol‐rich wastewater: the removal and inhibitory effect of sludge toxicity

Algas como bioindicadores da qualidade da água

The establishment of new protein expression system using N starvation inducible promoters in Chlorella

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