Chlorella minutissima-assisted silver nanoparticles synthesis and evaluation of its antibacterial activity

Abstract

The conventional methods of nanoparticles synthesis led to the production of highly toxic by-products and the use of toxic chemicals that are highly expensive in nature. Thus, the recent past has witnessed a surge in green synthesis of nanoparticles as a sustainable alternative. The present study outlines the biogenic silver nanoparticles (Ag-NPs) synthesis from an aqueous extract of Chlorella minutissima . The effect of certain parameters such as the reaction mixture’s pH and precursor metal solution to algal extract ratios were explored and optimized. The UV spectrophotometric analysis of Ag-NPs gave surface plasmon response maximally at 426 nm. The developed Ag-NPs were characterized using zeta potential, indicating their high stability (-21.2 mV) with a mean diameter of 73.13 nm. Results from field emission-scanning electron microscopy (FE-SEM) showed that the particles were spherical in shape. Ag-NPs synthesized using Chlorella minutissima extract could significantly inhibit the growth of both Gram-positive and Gram-negative bacterial species. The study highlights that using C. minutissima extract for Ag-NPs synthesis is a convenient and fast process for controlling the growth of Gram-positive as well as Gram-negative bacteria.

Sustainable Synthesis of Novel Green-Based Nanoparticles for Therapeutic Interventions and Environmental Remediation

“…For instance, biofabrication of various forms of NPs from the dried extracts of Chlorella vulgaris , a unicellular algae, aids in exploring its antibacterial potential against multidrug resistant pathogens . The phyco-synthesized AgNPs from Chlorella minutissima aqueous extract could potentially control bacterial growth …”

“…28 The phycosynthesized AgNPs from Chlorella minutissima aqueous extract could potentially control bacterial growth. 29 Conclusively, the synthesis of virus-mediated NPs has also gained enormous interest in the past decade. Due to their numerous chemical functions and a vast range of forms and sizes, viruses are superior to other biomolecules in many ways when it comes to the production of NPs.…”

Green Synthesis of Multifunctional Silver Nanoparticles Using Plectranthus amboinicus for Sensitive Detection of Triethylamine, with Potential In Vitro Antibacterial and Anticancer Activities

“…Antibiotic resistance is a serious issue faced by the health sector in recent years [36]. Nanoparticles have been demonstrated to be a promising candidate with potent antimicrobial activity, as their large surface-to-volume ratio helps in evading bacteria.…”