Chlorate Contamination in Commercial Growth Media as a Source of Phenotypic Heterogeneity within Bacterial Populations

Vincent, Maxence S.; Vergnes, Alexandra; Ezraty, Benjamin

doi:10.1128/spectrum.04991-22

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…The discovery of the bacterial adaptation mechanism to chlorate stress prompted us to employ an E. coli strain containing an msrP-lacZ fusion as a biosensor for detecting chlorate in commercial growth media for bacteriology. Through this approach, we were able to identify the presence of chlorate in some batches of Casamino Acids and agar [ 17 ]. The objective of this study was to enhance the sensitivity and efficiency of the bacterial chlorate biosensor by employing synthetic biology and optimized growth conditions of the strain.…”

Section: Introductionmentioning

confidence: 99%

Engineering of a Bacterial Biosensor for the Detection of Chlorate in Food

et al. 2023

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Chlorate can contaminate food due to the use of chlorinated water for processing or equipment disinfection. Chronic exposure to chlorate in food and drinking water is a potential health concern. The current methods for detecting chlorate in liquids and foods are expensive and not easily accessible to all laboratories, highlighting an urgent need for a simple and cost-effective method. The discovery of the adaptation mechanism of Escherichia coli to chlorate stress, which involves the production of the periplasmic Methionine Sulfoxide Reductase (MsrP), prompted us to use an E. coli strain with an msrP-lacZ fusion as a biosensor for detecting chlorate. Our study aimed to optimize the bacterial biosensor’s sensitivity and efficiency to detect chlorate in various food samples using synthetic biology and adapted growth conditions. Our results demonstrate successful biosensor enhancement and provide proof of concept for detecting chlorate in food samples.

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Section: Introductionmentioning

confidence: 99%