Chloramphenicol Loaded Microemulsions: Development, Characterization and Stability

Rashid, Muhammad A.; Naz, Tehreem; Abbas, Muhammad; Nazir, Sadia; Younas, Nafisa; Majeed, Sana; Qureshi, Naseem A.; Akhtar, Muhammad Nadeem

doi:10.1016/j.colcom.2018.11.006

Cited by 26 publications

(13 citation statements)

References 28 publications

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“…Considering the development of new formulations, microemulsions (MEs) have attracted great interest as potential drug delivery systems, mainly due to their unique physicochemical properties such as drug solubilization and enhanced absorption properties [10,11]. MEs are a thermodynamically stable, isotropic, transparent liquid system consisting of two immiscible liquids (usually water and oil) stabilized by a film of surfactant compounds, suitably combined with a cosurfactant [12,13]. The presence of the surfactant helps to reduce the interfacial tension, making it possible to join the oil and aqueous phases [14,15].…”

Section: Introductionmentioning

confidence: 99%

Development of a Method for the Quantification of Clotrimazole and Itraconazole and Study of Their Stability in a New Microemulsion for the Treatment of Sporotrichosis

et al. 2019

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Sporotrichosis occurs worldwide and is caused by the fungus Sporothrix brasiliensis. This agent has a high zoonotic potential and is transmitted mainly by bites and scratches from infected felines. A new association between the drugs clotrimazole and itraconazole is shown to be effective against S. brasiliensis yeasts. This association was formulated as a microemulsion containing benzyl alcohol as oil, Tween® 60 and propylene glycol as surfactant and cosurfactant, respectively, and water. Initially, the compatibility between clotrimazole and itraconazole was studied using differential scanning calorimetry (DSC), thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (PXRD). Additionally, a simple and efficient analytical HPLC method was developed to simultaneously determine the concentration of clotrimazole and itraconazole in the novel microemulsion. The developed method proved to be efficient, robust, and reproducible for both components of the microemulsion. We also performed an accelerated stability study of this formulation, and the developed analytical method was applied to monitor the content of active ingredients. Interestingly, these investigations led to the detection of a known clotrimazole degradation product whose structure was confirmed using NMR and HRMS, as well as a possible interaction between itraconazole and benzyl alcohol.

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Section: Introductionmentioning

confidence: 99%

Development of a Method for the Quantification of Clotrimazole and Itraconazole and Study of Their Stability in a New Microemulsion for the Treatment of Sporotrichosis

et al. 2019

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“…Another important character that should be assessed is the viscosity of the ophthalmic preparations, which affects the ocular contact time and thus the drainage of the drug by tears. It is clear from Table 6 that all formulations were found to exhibit viscosity values higher than the acceptable value of ophthalmic preparations (20 cP) [ 69 ], which were anticipated to increase the formulation residence time and hence its ocular bioavailability with keeping the advantage of reducing the instillation frequency per day [ 35 ]. The measured viscosities of the selected formulations were found to be affected by the water content, as water reduces the interaction between hydrophilic headgroups of the surfactant and thus decreases the viscosity [ 70 ].…”

Section: Resultsmentioning

confidence: 99%

Development of Triamcinolone Acetonide-Loaded Microemulsion as a Prospective Ophthalmic Delivery System for Treatment of Uveitis: In Vitro and In Vivo Evaluation

2021

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Treatment of uveitis (i.e., inflammation of the uvea) is challenging due to lack of convenient ophthalmic dosage forms. This work is aimed to determine the efficiency of triamcinolone acetonide (TA)-loaded microemulsion as an ophthalmic delivery system for the treatment of uveitis. Water titration method was used to construct different pseudo-ternary phase diagrams. Twelve microemulsion formulations were prepared using oleic acid, Cremophor EL, and propylene glycol. Among all tested formulations, Formulation F3, composed of oil: surfactant-co-surfactant (1:1): water (15:35:50% w/w, respectively), was found to be stable and showed acceptable pH, viscosity, conductivity, droplet size (211 ± 1.4 nm), and zeta potential (−25 ± 1.7 mV) and almost complete in vitro drug release within 24 h. The in vivo performance of the optimized formulation was evaluated in experimentally uveitis-induced rabbit model and compared with a commercial TA suspension (i.e., Kenacort®-A) either topically or by subconjunctival injection. Ocular inflammation was evaluated by clinical examination, white blood cell count, protein content measurement, and histopathological examination. The developed TA-loaded microemulsion showed superior therapeutic efficiency in the treatment of uveitis with high patient compliance compared to commercial suspension. Hence, it could be considered as a potential ocular treatment option in controlling of uveitis.

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“…The refractive index of all formulations was 1.332-1.371 and the pH 5.68 to 6.89. Conductivity results show that all microemulsions prepared are oil in water type [22]. The viscosity of all of the microemulsions varied between 14.7 and 29 mPas, which means they are more viscous than the regular ophthalmic solutions, which ensures that the ocular residence time and ocular bioavailability would be increased [23].…”

Section: Determination Of Physiochemical Parameters Of Microemulsionsmentioning

confidence: 96%

Preparation of Ophthalmic Microemulsions Containing Lithuanian Royal Jelly and Their Biopharmaceutical Evaluation

et al. 2021

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Royal jelly is a natural substance secreted by worker honeybees that possesses antioxidant, anti-inflammatory, and other biological activities. The purpose of this study was to formulate microemulsions with incorporated Lithuanian royal jelly for possible ophthalmic delivery and to evaluate the quality of the microemulsions in vitro. The oil in water type microemulsions were prepared by the oil titration method, incorporating royal jelly, surfactant, co-surfactant, oil, and water. Physicochemical characteristics of the microemulsions and the quantity of 10-hydroxy-2-decenoic acid released in vitro were assessed. The in vitro assessment of prepared microemulsions formulations was performed with the Statens Seruminstitut rabbit cornea (SIRC) cell culture model. The results revealed that the droplet size of all microemulsion formulations was 67.88–124.2 nm and the polydispersity index was lower than 0.180. In the in vitro release study, the release of 10-hydroxy-2-decenoic acid depended on the amount of royal jelly incorporated and on the ratio of surfactant and co-surfactant in formulations. The in vitro tests with the SIRC cell culture line have shown that all formulations were found non-irritating.

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Chloramphenicol Loaded Microemulsions: Development, Characterization and Stability

Cited by 26 publications

References 28 publications

Development of a Method for the Quantification of Clotrimazole and Itraconazole and Study of Their Stability in a New Microemulsion for the Treatment of Sporotrichosis

Development of a Method for the Quantification of Clotrimazole and Itraconazole and Study of Their Stability in a New Microemulsion for the Treatment of Sporotrichosis

Development of Triamcinolone Acetonide-Loaded Microemulsion as a Prospective Ophthalmic Delivery System for Treatment of Uveitis: In Vitro and In Vivo Evaluation

Preparation of Ophthalmic Microemulsions Containing Lithuanian Royal Jelly and Their Biopharmaceutical Evaluation

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