Chlamydophila pneumoniae and Mycoplasma pneumoniae were not identified in sinus mucosa of patients with chronic rhinosinusitis

Abstract

Chronic rhinosinusitis is a symptomatic inflammation of the mucosa of the nose and paranasal sinuses lasting for at least 12 weeks. Atypical bacteria Chlamydophila pneumoniae and Mycoplasma pneumoniae are important causes of human respiratory tract infection. Also, they were identified in bronchial respiratory epithelium of patients with chronic obstructive pulmonary disease or asthma. Having in mind the unified airway concept, it is also possible that these bacteria can cause persistent infection of sinus mucosa in patients with chronic rhinosinusitis. Sixty consecutive patients with chronic rhinosinusitis who underwent the functional endoscopic sinus surgery due to medical therapy failure were included in the study. During the operation, sinuses were irrigated with sterile 0.9% NaCl solution and this lavage was immediately aspirated. Aspirates were used for the detection of C. pneumoniae and M. pneumoniae DNA using real-time PCR. C. pneumoniae and M. pneumoniae DNA were not detected in samples analysed. Atypical bacteria C. pneumoniae and M. pneumoniae did not cause persistent infection of sinus mucosa in patients with chronic rhinosinusitis.

Mycoplasma pneumoniaeandChlamydophila pneumoniae: a comparative study in patients with nasal polyposis and healthy controls

“…Given the intracellular life cycle of C pneumoniae and the documented increased presence of amplification inhibitors in aspirates 18 compared to tissue specimens, this would have influenced the study findings. Our study confirms the findings of Pandak et al ., 13 who argued against C pneumoniae involvement in the pathogenesis of chronic rhinosinusitis with nasal polyps (Table II).…”

“…They found no evidence of M pneumoniae -specific DNA encoding of the 16S ribosomal RNA gene in any of the tissues. Recently, Pandak et al 13 . used real-time polymerase chain reaction in sinus lavage aspirates but failed to detect any M pneumoniae DNA in 60 chronic rhinosinusitis patients (24 chronic rhinosinusitis without nasal polyps, 36 chronic rhinosinusitis with nasal polyps).…”

“…A few studies have investigated the potential role of M pneumoniae 11 – 14 and C pneumoniae 9 , 13 , 15 – 17 in chronic rhinosinusitis with nasal polyps using different techniques, although their results are controversial. In our study, we used real-time polymerase chain reaction to evaluate and compare the prevalence of M pneumoniae and C pneumoniae DNA in the nasal tissue samples of patients with chronic rhinosinusitis with nasal polyps and in those from healthy controls.…”

Mycoplasma pneumoniae from the Respiratory Tract and Beyond

“…However, there was no control group for comparison (77). M. pneumoniae is also uncommon in other upper respiratory conditions, such as otitis media, rhinosinusitis, and the common cold (78,79).…”

Pediatric Rhinosinusitis

“…However, unlike other chronic respiratory diseases, a study by Pandaket al on 60 patients with CRS showed that the sinus aspirates of patients with CRS didn't grow any of the atypical organisms (Mycoplasma pneumonia and Chlamydia pneumonia) that are frequently found in the chronic respiratory conditions (i.e. : COPD , asthma) [16].…”