Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress

Esperanza, Marta; Seoane, Marta; Rioboo, Carmen; Herrero, Concepción; Cid, Ángeles

doi:10.1016/j.aquatox.2015.05.012

Cited by 40 publications

(27 citation statements)

References 59 publications

(70 reference statements)

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“…Obtained results indicated a reduction of photosynthetic activity and this is related to the atrazine mechanism of action on the D1 protein of PSII ( Chalifour et al, 2009 andDidur et al, 2012). Photosynthesis inhibition induced by atrazine toxicity will affect the entire physiological state and cell growth process ( Bi Fai et al, 2007 andEsperanza et al, 2015).…”

Section: Photosynthetic Pigment Content and Chlorophyll A Fluorescencementioning

confidence: 99%

“…RNA was isolated from control and atrazine exposed (0.25 μM) cells after 3 h of atrazine exposure, using the reagent NZYol (NZYTech), following the procedure described previously in Esperanza et al (2015).…”

Section: Total Rna Extraction and Rna-seqmentioning

confidence: 99%

“…Currently, with the development of the omics, new alternatives arise to study the effects of pollutants on microalgae (Dowling and Sheehan, 2006, Esperanza et al, 2015,Jamers et al, 2009and Monsinjon and Knigge, 2007. Transcriptomics and proteomics can be used to detect and characterize responses to external stimulus, and have a great potential for investigating stress mechanisms and responses affecting growth and other physiological and biochemical endpoints (Jamers and De Coen, 2010, Nestler et al, 2012and Subramanian et al, 2014, being increasingly applied in ecotoxicology and other fields of biological science.…”

Section: Introductionmentioning

confidence: 99%

“…Previous research has demonstrated that C. reinhardtii cells exposed to atrazine change their metabolism and get energy mainly by heterotrophic pathways ( Esperanza et al, 2015). In a global climate change scenario with a dramatic increase in CO2 levels, it is very important to know the behavior of carbon fixing organisms.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study

Esperanza

Seoane

Rioboo

et al. 2016

Science of The Total Environment

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Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3h. Physiological cellular parameters, such as chlorophyll a fluorescence and oxidative stress monitored by flow cytometry and pigments levels were altered in microalgal cells exposed to 0.25 μM of atrazine. Furthermore, the effects of this herbicide on C. reinhardtii were explored using "omics" techniques. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 9 differentially expressed genes, related to photosynthesis, between control cultures and atrazine exposed cultures. Proteomic profiles were obtained using iTRAQ tags and MALDI-MS/MS analysis, identifying important changes in the proteome during atrazine stress; 5 proteins related to photosynthesis were downexpressed. The results of these experiments advance the understanding of photosynthetic adjustments that occur during an early herbicide exposure. Inhibition of photosynthesis induced by atrazine toxicity will affect the entire physiological and biochemical states of microalgal cells.

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Section: Photosynthetic Pigment Content and Chlorophyll A Fluorescencementioning

confidence: 99%

Section: Total Rna Extraction and Rna-seqmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study

Esperanza

Seoane

Rioboo

et al. 2016

Science of The Total Environment

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“…However, NR activity was not significantly affected by the herbicide glufosinate in the marine diatom Phaeodactylum tricornutm (Xie et al, 2014). Transcriptomic analyses carried out by RNA-Seq technique in C. reinhardtii cells exposed to atrazine have also shown a downregulation of NIT1 gene which encodes a nitrate reductase (Esperanza et al, 2015).…”

Section: Discussionmentioning

confidence: 97%

Atrazine induced changes in elemental and biochemical composition and nitrate reductase activity in Chlamydomonas reinhardtii

Fernández-Naveira

Rioboo

Cid

et al. 2016

European Journal of Phycology

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Herbicides play an important role in agricultural practices but the introduction of these compounds into the aquatic environment can have severe consequences for non-target organisms such as microalgae. The ubiquitous green freshwater microalga Chlamydomonas reinhardtii, a model species in all aspects of microalgal physiology, was used to assess the toxicity of atrazine, one of the most widely used herbicides throughout the world. Atrazine acts on photosynthesis and therefore can affect non-target primary producers, such as microalgae.Growth, dry weight, elemental composition, photosynthetic pigments and protein contents and nitrate reductase activity were studied. After 96 h of exposure to different atrazine concentrations all the parameters studied were affected, but different sensitivities to the herbicide were shown. Nitrate reductase (NR) activity was strongly affected even at an atrazine concentration that did not affect growth (0.1 µM); the lowest concentrations of atrazine assayed (0.1 and 0.25 µM) provoked a > 40% decrease in NR activity and NR decreased > 80% with atrazine concentrations of 0.5 µM. C/N ratio was also affected by all the atrazine concentrations assayed. Nitrate reductase activity and C/N ratio were better indicators of the cellular stress state than data on other biochemical components or growth rate. Among cell parameters assayed, the NR activity stood out as a sensitive cytotoxicity endpoint and the activity of this enzyme can be suggested as a sensitive biomarker of stress induced by atrazine in C. reinhardtii.

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Transcriptional and cellular effects of benzotriazole UV stabilizers UV‐234 and UV‐328 in the freshwater invertebrates Chlamydomonas reinhardtii and Daphnia magna

Giraudo

Cottin

Esperanza

et al. 2017

Enviro Toxic and Chemistry

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Benzotriazole ultra violet stabilizers (BZT-UVs) are compounds used in many applications and products to prevent photochemical degradation. Despite their widespread presence in aquatic ecosystems and persistence in the environment, there are very limited data on their effects and toxicity, and their modes of action remain largely unknown. The objectives of the present study were to evaluate the chronic effects of 2 BZT-UVs, 2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (UV-234) and 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV-328), on the freshwater green algae Chlamydomonas reinhardtii and the freshwater crustacean Daphnia magna. Organisms were exposed to 0.01 and 10 μg/L of UV-234, UV-328, as well as a mixture of the 2 compounds. Life-history endpoints (viability, reproduction, and growth) and oxidative stress-related biomarkers (gene transcription, reactive oxygen species [ROS] production, and lipid peroxidation) were measured. Daphnia magna growth, reproduction, and gene transcription were not impacted by 21-d individual or mixed exposure. After 96-h of exposure, no differences were observed on the cellular viability of C. reinhardtii for either of the 2 BZT-UVs. In the algae, results showed increased ROS production in response to UV-328 and lipid peroxidation following exposure to UV-234. Synergistic effects of the 2 BZT-UVs were evident at the transcriptional level with 2 to 6 times up-regulation of glutathione peroxidase (gp ) in response to the mixture for all treatment conditions. The transcription of superoxide dismutase (sod), catalase (cat), and ascorbic peroxidase (apx) was also regulated by UV-234 and UV-328 in the green algae, most likely as a result of ROS production and lipid peroxidation. Results from the present study suggest potential impacts of UV-234 and UV-328 exposure on the antioxidant defense system in C. reinhardtii. Environ Toxicol Chem 2017;36:3333-3342. © 2017 Crown in the Right of Canada. Published by Wiley Periodicals Inc., on behalf of SETAC.

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Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress

Cited by 40 publications

References 59 publications

Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study

Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study

Atrazine induced changes in elemental and biochemical composition and nitrate reductase activity in Chlamydomonas reinhardtii

Transcriptional and cellular effects of benzotriazole UV stabilizers UV‐234 and UV‐328 in the freshwater invertebrates Chlamydomonas reinhardtii and Daphnia magna

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