Chlamydomonas cell cycle mutant crcdc5 over-accumulates starch and oil

Torres-Romero, Ismael; Kong, Fantao; Légeret, Bertrand; Beisson, Fred; Peltier, Gilles; Li‐Beisson, Yonghua

doi:10.1016/j.biochi.2019.09.017

Cited by 16 publications

(20 citation statements)

References 49 publications

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“…The same effect has been observed in other microalgae. In the green alga Chlamydomonas reinhardtii both lipids and starch contents (storage carbohydrate in algae and equivalent to chrysolaminarin in diatoms) increased in cultures grown at 5% (50,000 ppm) [51] and at 2% (20,000 ppm) CO 2 [52], as well as in Chlorella sorokiniana grown at 2% CO 2 [53]. It is possible that in some microalgae CO 2 treatment can have a synergistic effect on the accumulation of both storage compounds.…”

Section: Discussionmentioning

confidence: 99%

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Jensen

Yangüez

Carrière

et al. 2019

Biology

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Accumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO2 supply can also be used to improve both lipids and carbohydrates accumulation. Given the high diversity among diatoms, we studied the two marine model diatoms—Thalassiosira pseudonana and Phaeodactylum tricornutum, a freshwater diatom, Asterionella formosa, and Navicula pelliculosa—found in fresh- and sea-water environments. We measured the accumulation of reserve compounds and the activity of enzymes involved in carbon metabolism in these diatoms grown at high and atmospheric CO2. We observed that biomass and lipid accumulation in cells grown at high CO2 differ among the diatoms. Lipid accumulation increased only in P. tricornutum and N. pelliculosa grown in seawater in response to elevated CO2. Moreover, accumulation of lipids was also accompanied by an increased activity of the enzymes tested. However, lipid accumulation and enzyme activity decreased in N. pelliculosa cultured in fresh water. Chrysolaminarin accumulation was also affected by CO2 concentration; however, there was no clear relation with lipids accumulation. Our results are relevant to understand better the ecological role of the environment in the diatom adaptation to CO2 and the mechanisms underpinning the production of storage compounds considering diatom diversity.

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Section: Discussionmentioning

confidence: 99%

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Jensen

Yangüez

Carrière

et al. 2019

Biology

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“…More recently, a Myb-like transcription factor Phosphorus Starvation Response (PtPSR) was identified in P. tricornutum; its modulation might represent a good strategy to enhance cell growth and TAG production in limited-phosphorous conditions [153]. A MYB DNA binding protein involved in cell cycle regulation was instead targeted in Chlamydomonas, showing that the mutants devoid of CrCDC5 accumulate more oil and starch with respect to WT [154].…”

Section: Engineering Of the Lipid Biosynthesis For Renewable Energiesmentioning

confidence: 99%

Potential and Challenges of Improving Photosynthesis in Algae

Vecchi

Barera

Bassi

et al. 2020

Plants

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Sunlight energy largely exceeds the energy required by anthropic activities, and therefore its exploitation represents a major target in the field of renewable energies. The interest in the mass cultivation of green microalgae has grown in the last decades, as algal biomass could be employed to cover a significant portion of global energy demand. Advantages of microalgal vs. plant biomass production include higher light-use efficiency, efficient carbon capture and the valorization of marginal lands and wastewaters. Realization of this potential requires a decrease of the current production costs, which can be obtained by increasing the productivity of the most common industrial strains, by the identification of factors limiting biomass yield, and by removing bottlenecks, namely through domestication strategies aimed to fill the gap between the theoretical and real productivity of algal cultures. In particular, the light-to-biomass conversion efficiency represents one of the major constraints for achieving a significant improvement of algal cell lines. This review outlines the molecular events of photosynthesis, which regulate the conversion of light into biomass, and discusses how these can be targeted to enhance productivity through mutagenesis, strain selection or genetic engineering. This review highlights the most recent results in the manipulation of the fundamental mechanisms of algal photosynthesis, which revealed that a significant yield enhancement is feasible. Moreover, metabolic engineering of microalgae, focused upon the development of renewable fuel biorefineries, has also drawn attention and resulted in efforts for enhancing productivity of oil or isoprenoids.

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“…A posttranslational regulation strategy has been applied to E. coli to decouple biomass accumulation and poly(3-hydroxybutyrate) synthesis (Durante-Rodríguez et al, 2018). Mutations that delay or arrest cell cycle progression (Madeira et al, 2019;Torres-Romero et al, 2020) have also been found to increase cellular oil production and therefore stand to be reasonable targets for genetic engineering in other organisms. These achievements suggest that at least some host organisms may be amenable to engineering strategies that effectively redirect carbon flux away from biomass accumulation and toward product synthesis to maximize fatty alcohol yields.…”

Section: Prospectivesmentioning

confidence: 99%

Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations

Krishnan

McNeil

Stuart

2020

Front. Bioeng. Biotechnol.

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Concerns about climate change and environmental destruction have led to interest in technologies that can replace fossil fuels and petrochemicals with compounds derived from sustainable sources that have lower environmental impact. Fatty alcohols produced by chemical synthesis from ethylene or by chemical conversion of plant oils have a large range of industrial applications. These chemicals can be synthesized through biological routes but their free forms are produced in trace amounts naturally. This review focuses on how genetic engineering of endogenous fatty acid metabolism and heterologous expression of fatty alcohol producing enzymes have come together resulting in the current state of the field for production of fatty alcohols by microbial cell factories. We provide an overview of endogenous fatty acid synthesis, enzymatic methods of conversion to fatty alcohols and review the research to date on microbial fatty alcohol production. The primary focus is on work performed in the model microorganisms, Escherichia coli and Saccharomyces cerevisiae but advances made with cyanobacteria and oleaginous yeasts are also considered. The limitations to production of fatty alcohols by microbial cell factories are detailed along with consideration to potential research directions that may aid in achieving viable commercial scale production of fatty alcohols from renewable feedstock.

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Chlamydomonas cell cycle mutant crcdc5 over-accumulates starch and oil

Cited by 16 publications

References 49 publications

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Potential and Challenges of Improving Photosynthesis in Algae

Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations

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