Chlamydial biology and its associated virulence blockers

Beeckman, Delphine Sylvie Anne; Puysseleyr, Leentje De; Puysseleyr, Kristien De; Vanrompay, Daisy

doi:10.3109/1040841x.2012.726210

Cited by 5 publications

(4 citation statements)

References 200 publications

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“…More than 100 million new cases were estimated of C. trachomatis STIs in 2008, while >1 million people are estimated to have lost their vision as a result of the damage accrued through repeated trachoma. , This Gram-negative obligate intracellular pathogen is characterized by a biphasic developmental cycle that switches between an infectious elementary body (EB) and the replicative intracellular reticulate body (RB). , Infection is initiated by EBs which adhere to and invade the host cell, transition to the RB form, and replicate within a vacuole known as the inclusion. RBs subsequently differentiate back to infectious EBs, which after lysis of the inclusion and exiting the host cell, propagate the infection.…”

Section: Introductionmentioning

confidence: 99%

Thiazolino 2-Pyridone Amide Inhibitors of Chlamydia trachomatis Infectivity

et al. 2016

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The bacterial pathogen Chlamydia trachomatis is a global health burden currently treated with broad-spectrum antibiotics which disrupt commensal bacteria. We recently identified a compound through phenotypic screening that blocked infectivity of this intracellular pathogen without host cell toxicity (compound 1, KSK 120). Herein, we present the optimization of 1 to a class of thiazolino 2-pyridone amides that are highly efficacious (EC50 ≤ 100 nM) in attenuating infectivity across multiple serovars of C. trachomatis without host cell toxicity. The lead compound 21a exhibits reduced lipophilicity versus 1 and did not affect the growth or viability of representative commensal flora at 50 μM. In microscopy studies, a highly active fluorescent analogue 37 localized inside the parasitiphorous inclusion, indicative of a specific targeting of bacterial components. In summary, we present a class of small molecules to enable the development of specific treatments for C. trachomatis.

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Section: Introductionmentioning

confidence: 99%

Thiazolino 2-Pyridone Amide Inhibitors of Chlamydia trachomatis Infectivity

et al. 2016

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“…, or interfering with the quorum sensing of Pseudomanas aeruginosa , Staphylococcus aureus , etc. [ 61 , 62 , 63 ]. This approach may pave the way to new molecules targeting the virulence determinants involved in C. pneumoniae -induced oxidative stress, thus inhibiting the development and progression of atherosclerotic process.…”

Section: C Pneumoniae -Induced Oxidative Stresmentioning

confidence: 99%

Chlamydia pneumoniae and Oxidative Stress in Cardiovascular Disease: State of the Art and Prevention Strategies

Pietro¹,

Filardo²,

Santis³

et al. 2014

IJMS

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Chlamydia pneumoniae, a pathogenic bacteria responsible for respiratory tract infections, is known as the most implicated infectious agent in atherosclerotic cardiovascular diseases (CVDs). Accumulating evidence suggests that C. pneumoniae-induced oxidative stress may play a critical role in the pathogenesis of CVDs. Indeed, the overproduction of reactive oxygen species (ROS) within macrophages, endothelial cells, platelets and vascular smooth muscle cells (VSMCs) after C. pneumoniae exposure, has been shown to cause low density lipoprotein oxidation, foam cell formation, endothelial dysfunction, platelet adhesion and aggregation, and VSMC proliferation and migration, all responsible for the typical pathological changes of atherosclerotic plaque. The aim of this review is to improve our insight into C. pneumoniae-induced oxidative stress in order to suggest potential strategies for CVD prevention. Several antioxidants, acting on multi-enzymatic targets related to ROS production induced by C. pneumoniae, have been discussed. A future strategy for the prevention of C. pneumoniae-associated CVDs will be to target chlamydial HSP60, involved in oxidative stress.

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“…C. abortus presents a biphasic replicative cycle, consisting of an extracellular infectious form called the elementary body (EC) and an intracellular form, identified as the reticular body (CR). ECs are spherical structures about 0.2-0.3 µm in diameter, antigenic, non-proliferative, responsible for carrying out intracellular adhesion, fusion, and infection (Beeckman et al, 2014). The RCs are the metabolically active intracellular form of the bacterium, they are structures that measure approximately 0.5 -2 mm in diameter.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Chlamydia Abortus in captive ruminants of Mexico

Manjarrez-Pompa,

Jiménez,

Palacio

et al. 2024

Braz. J. Anim. Environ. Res.

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Background. Chlamydia abortus is the etiological agent of ovine enzootic abortion and is the most common cause of reproductive failure in ruminants. In Mexico, the prevalence of anti-Chlamydia abortus IgG antibody was determined by ELISA assay and the molecular identification by PCR in sheep with a clinical history of abortion. However, information regarding the prevalence of C. abortus infections in wild mammals is unknown. Objective. The study aimed to determine the seroprevalence of C. abortus using a specific recombinant ELISA followed by the identification of risk factors associated with captive ruminants in the Zacango Zoo and Ocotal Park, Mexico. Methodology. A total of 103 specimens corresponding to 15 different species of captive ruminants at the Zacango Zoo and Parque Ocotal, Mexico, were tested to determine the presence in sera of antibodies against C. abortus using a specific recombinant ELISA kit. In addition, the influence of risk factors such as age, sex, and the site, were evaluated on seropositivity. Results. Seropositive species included bighorn sheep (Ovis canadensis) (1/2), barbary sheep (Ammotragus lervia) (4/23), red deer (Cervus elaphus) (1/2), and a domestic goat (Capra hircus) (1/3). A total frequency of 7/103 (6.8%) was obtained. The analysis of antibody values about risk factors showed that the older animals (3.1 years) were seropositive with a frequency of 7/80 (8.7%) and three males (3/47) and four females (4/56) also were seropositive; however, no statistical significance was observed. Seropositive specimens from Ocotal Park (5/28) showed a higher risk compared to Zacango Zoo. Conclusion. The prevalence of C. abortus in captive ruminants at both sites was low; however, the health impacts to these species are unknown. Further research is warranted on the transmission of C. abortus within captive ruminants, and diagnostic of domestic livestock, and humans.

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Chlamydial biology and its associated virulence blockers

Cited by 5 publications

References 200 publications

Thiazolino 2-Pyridone Amide Inhibitors of Chlamydia trachomatis Infectivity

Thiazolino 2-Pyridone Amide Inhibitors of Chlamydia trachomatis Infectivity

Chlamydia pneumoniae and Oxidative Stress in Cardiovascular Disease: State of the Art and Prevention Strategies

Seroprevalence of Chlamydia Abortus in captive ruminants of Mexico

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