Chlamydiae (with Phages), Mycoplasmas, and Rickettsiae in Chesapeake Bay Bivalves

Jc, Harshbarger; Chang, Shi-Chuan

doi:10.1126/science.193184

Cited by 129 publications

(90 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar organisms have been reported from clams (Mya sp.) by Harshbarger et al (1977) in Chesapeake Bay, in clams by Buchanan (1978) from the coast of Scotland, and by Comps et al (1977Comps et al ( , 1978 in oysters and clams from France.…”

Section: Infectious Disordersmentioning

confidence: 99%

Prospective study of infectious and noninfectious diseases in oysters and fishes in three Gulf of Mexico estuaries

Ja¹

1985

Dis. Aquat. Org.

View full text Add to dashboard Cite

Section: Infectious Disordersmentioning

confidence: 99%

Prospective study of infectious and noninfectious diseases in oysters and fishes in three Gulf of Mexico estuaries

Ja¹

1985

Dis. Aquat. Org.

View full text Add to dashboard Cite

“…The question of what role freshwater bivalves may play as alternate hosts or reservoirs for the spread of vertebrate rickettsial or chlamydial diseases was first raised by Harshbarger et al (1977). Buchanan (1978) did have limited success in culturing an intracellular prokaryote from a marine intertidal bivalve in hens' eggs, and this provided some evidence that such prokaryotes might truly have an alternate host in birds, but no other similar studies have been reported since.…”

Section: Future Researchmentioning

confidence: 99%

Characterization of intracytoplasmic prokaryote infections in Dreissena sp. (Bivalvia: Dreissenidae)

Giambérini²,

Jf³

et al. 2001

Dis. Aquat. Org.

View full text Add to dashboard Cite

This study characterizes intracytoplasmic infections with prokaryote microorganisms in Dreissena sp. (near Dreissena polymorpha) from northeastern Greece and represents the first report of such infections in freshwater bivalves. Light microscope observations of stained tissues revealed basophilic, cytoplasmic inclusion bodies in 87.5% (28/32) of the mussels sectioned. Inclusions in epithelial cells and connective tissues were noted, respectively, in 34.4 and 71.9% of the sample, with 5 mussels (15.6%) having both tissue types infected. Epithelial cell infections were observed in histological sections only in digestive gland tubules and ducts; within tubules, inclusions were present more often in secretory than digestive cells. Connective tissue infections, however, were systemic; among the 32 mussels sectioned, inclusions were found in the gills (65.6%), foot (12.5%), mantle (9.4%), labial palps (6.3%), digestive gland (6.3%), stomach (6.3%), and gonads (3.1%). Cytoplasmic inclusions (maximum dimension, 138 µm) were prominent enough in the gills to be visible in 17.0% of the 247 mussels dissected. Ultrastructurally, prokaryote cells in gill connective tissues were clearly characteristic of Chlamydiales-like organisms, with each intracytoplasmic inclusion containing a loosely packed mixture of elementary, reticulate, intermediate bodies, and blebs. Prokaryote colonies in digestive gland epithelial cells exclusively contained 1 of 4 morphological cell types and were considered Rickettsiales-like. Hexagonal, virus-like particles were present in the cytoplasm of the largest of these Rickettsiales-like prokaryotes. Although host stress was evident from localized cell necrosis and dense hemocyte infiltration, overall infection was fairly benign, with no major, adverse impact on body condition evident among sectioned or dissected mussels. A possible negative effect was partial constriction of gill water tubes, but at the infection intensity observed (typical range 1 to 7 inclusion bodies per section), significant interference with respiration and other metabolic functions of the gills was highly unlikely.

show abstract

“…To survive and reproduce, the rickettsia organism must enter the eucaryotic host cell, multiply within the cell, exit and reestablish this cycle in another cell (Winkler 1990). Rickettsia-like microorganisms infect a wide range of aquatic crustaceans including amphipods, crabs, freshwater crayfish and prawns (Federici et al 1974, Harshbarger et al 1977, Bonami & Pappalardo 1980, Larrson 1982, Johnson 1984, Brock et al 1986, Anderson et al 1987, Krol et al 1991, Frelier et al 1992, Fryer & Lan-nan 1994, Bower et al 1996, Jimenez & Romero 1997, Edgerton & Prior 1999, Wang & Gu 2002.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection methods developed for a systemic rickettsia-like bacterium (RLB) in Penaeus monodon (Decapoda: Crustacea)

Nunan¹,

Poulos²,

Redman³

et al. 2003

Dis. Aquat. Org.

View full text Add to dashboard Cite

Molecular detection methods were developed to aid in the diagnosis of a rickettsia-like bacterium (RLB) which caused severe mortalities of farm-raised Penaeus monodon in Madagascar. Using primers derived from the 16S rRNA gene of bacteria, a PCR assay was optimized to amplify this region of the genome of the RLB, using extracted DNA from infected P. monodon tissue as the template. The resulting amplified PCR product was sequenced and 2 novel primers were selected from the variable region of the gene. These primers amplified a 532 bp fragment of DNA originating from the rickettsia-infected samples. The PCR assay was optimized and tested on DNA extracted from specific pathogen-free (SPF) P. vannamei tissue and several other strains of bacteria. The PCR assay with the rickettsia-specific primers was specific for this RLB and did not amplify the other DNA samples tested. The 532 bp PCR-amplified fragment was labeled with digoxigenin (DIG) for in situ hybridization assays. This probe was tested on SPF, RLB and bacteria-infected shrimp specimens preserved in Davidson's fixative. The probe was specific for both natural and experimental rickettsial infections. Hybridization with this probe required a stringent temperature of 65°C, otherwise cross-reactivity was observed with other types of bacteria. KEY WORDS: Rickettsia · Penaeus monodon · Shrimp · CrustaceaResale or republication not permitted without written consent of the publisher

show abstract

Chlamydiae (with Phages), Mycoplasmas, and Rickettsiae in Chesapeake Bay Bivalves

Cited by 129 publications

References 1 publication

Prospective study of infectious and noninfectious diseases in oysters and fishes in three Gulf of Mexico estuaries

Prospective study of infectious and noninfectious diseases in oysters and fishes in three Gulf of Mexico estuaries

Characterization of intracytoplasmic prokaryote infections in Dreissena sp. (Bivalvia: Dreissenidae)

Molecular detection methods developed for a systemic rickettsia-like bacterium (RLB) in Penaeus monodon (Decapoda: Crustacea)

Contact Info

Product

Resources

About