Chlamydia trachomatis serovar L2 infection model using human lymphoid Jurkat cells

Kubo, Takeru; Ishida, Koichi; Matsuo, Junji; Nakamura, Shinji; Hayashi, Yasuhiko; Sakai, Haruna; Yoshida, Mitsutaka; Takahashi, Kaori; Hirai, Itaru; Yamamoto, Yoshimasa; Yamaguchi, Hiroyuki

doi:10.1016/j.micpath.2012.02.005

Cited by 9 publications

(3 citation statements)

References 48 publications

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“…Further, although several studies already investigated the survival of Chlamydia spp. in myeloid cells [39][40][41][42], the role of lymphocytic cells as survival niche for replication and dissemination of Ct was not examined extensively [33]. Therefore, in addition to the role of TLRs, another aim of our study was to address the importance and capacity of lymphocytic cells for recognition, uptake and survival of Ct in comparison to myeloid cells.…”

Section: Discussionmentioning

confidence: 99%

“…To identify and analyze the signaling function of the different cell surface TLRs that recognize bacterial cell wall products, i.e., TLR1/2, TLR2/6, TLR4, TLR4/CD14 and TLR5 upon recognition of Ct, we used the monocytic cell line THP-1 and the T-cell line Jurkat. Both cell lines, previously described as potential host cells for Ct serovar L2 [33,34], were transduced with a construct encoding eGFP under the control of a NF-B response element as described earlier by us [27]. THP-1 cells express constitutively TLR1/2, TLR2/6 and TLR5, accordingly, the reporters responded towards the corresponding TLR ligands Pam3CSK4, FSL-1 and flagellin in the picomolar range [28,29].…”

Section: Identification and Analysis Of Tlrs For Ct Recognitionmentioning

confidence: 99%

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TLR4/CD14/MD2 Revealed as the Limited Toll-like Receptor Complex for Chlamydia trachomatis-Induced NF-κB Signaling

et al. 2022

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Chlamydia trachomatis (Ct) is the most common cause of genital tract infections as well as preventable blindness worldwide. Pattern recognition receptors such as toll-like receptors (TLRs) represent the initial step in recognizing pathogenic microorganisms and are crucial for the initiation of an appropriate immune response. However, our understanding of TLR-signaling in Chlamydia-infected immune cells is incomplete. For a better comprehension of pathological inflammatory responses, robust models for interrogating TLR-signaling upon chlamydial infections are needed. To analyze the TLR response, we developed and utilized a highly sensitive and selective fluorescent transcriptional cellular reporter system to measure the activity of the transcription factor NF-κB. Upon incubation of the reporter cells with different preparations of Ct, we were able to pinpoint which components of TLRs are involved in the recognition of Ct. We identified CD14 associated with unique characteristics of different serovars as the crucial factor of the TLR4/CD14/MD2 complex for Ct-mediated activation of the NF-κB pathway. Furthermore, we found the TLR4/CD14/MD2 complex to be decisive for the uptake of Ct-derived lipopolysaccharides but not for infection and replication of Ct. Imaging flow cytometry provided information about inclusion formation in myeloid- as well as lymphocytic cells and was highest for Ct L2 with at least 25% of inclusion forming cells. Ct E inclusion formation was eminent in Jurkat cells without CD14 expression (11.1%). Thus, our model enables to determine Ct uptake and signal induction by pinpointing individual components of the recognition and signaling pathways to better understand the immune response towards infectious pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Identification and Analysis Of Tlrs For Ct Recognitionmentioning

confidence: 99%

TLR4/CD14/MD2 Revealed as the Limited Toll-like Receptor Complex for Chlamydia trachomatis-Induced NF-κB Signaling

et al. 2022

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“…La Ct es una bacteria Gram negativa intracelular obligada (7,8) que causa la infección bacteriana de transmisión sexual (ITS) más común en el mundo; encontrando con cerca de 92 millones de casos de infección al año (2,(12)(13)(14). Existen distintas serovariedades: las causantes de tracoma (A-C), causantes de infecciones transmitidas por vía sexual (D-K) y las que causan linfogranuloma venéreo "LGV" (L1-L2-L3) (15,16).…”

Section: Generalidades De Chlamydia Spunclassified

Regulación de la familia de proteínas BCL-2 en células infectadas con Chlamydia trachomatis

Shubach

Galindo

Mora

2015

nova

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El presente artículo revisa los mecanismos inhibitorios de la apoptosis usados por Chlamydia trachomatis (Ct) frente a la familia de proteínas Bcl-2, para lograr su supervivencia intracelular. Chlamydia trachomatis es una bacteria intracelular obligada Gram negativa responsable de la infección de transmisión sexual más común en el mundo; este microorganismo es capaz de inhibir la apoptosis de la célula huésped durante su ciclo de desarrollo, obteniendo un refugio seguro para su supervivencia.Frente a estímulos como la infección de la célula por un patógeno, la familia de proteínas Bcl-2 regula la apoptosis por medio de la liberación del citocromo c de la mitocondria para desencadenar la muerte celular programada (MCP). Ct usa diversos mecanismos de regulación antiapoptótica para sobrevivir dentro de la célula huésped; dentro de ellos se observa la secreción de proteínas tales como CPAF y CADD, la escisión de la proteína Bid, el secuestro de Bad y en general el bloqueo de proteínas pertenecientes a la familia Bcl-2 con dominio BH3, que afectan directamente la vía mitocondrial ocasionando la persistencia, desarrollo y replicación de Ct en la célula huésped.

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Cell Lines as In Vitro Model for Studying Microbial Pathogenesis

Chattopadhyay

2020

Model Organisms for Microbial Pathogenesis, Biofilm Formation and Antimicrobial Drug Discovery

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Chlamydia trachomatis serovar L2 infection model using human lymphoid Jurkat cells

Cited by 9 publications

References 48 publications

TLR4/CD14/MD2 Revealed as the Limited Toll-like Receptor Complex for Chlamydia trachomatis-Induced NF-κB Signaling

TLR4/CD14/MD2 Revealed as the Limited Toll-like Receptor Complex for Chlamydia trachomatis-Induced NF-κB Signaling

Regulación de la familia de proteínas BCL-2 en células infectadas con Chlamydia trachomatis

Cell Lines as In Vitro Model for Studying Microbial Pathogenesis

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