Chlamydia trachomatis prevalence, genotype distribution and identification of the new Swedish variant in Southern Germany

Fieser, N.; Simnacher, Ulrike; Tausch, Yvonne; Werner-Belak, S.; Ladenburger-Strauß, S.; Baum, Heike von; Reischl, Udo; Essig, Andreas

doi:10.1007/s15010-012-0301-2

Cited by 16 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In an observational study performed in 2004 among sexually experienced females aged 14-20 years in Berlin, Germany, CT-positivity of 6.5% (95% CI: 4.7-9.0) was found [24]. In a study performed in 2008-09 in a mid-sized town in Germany, a prevalence of 4.2% was found in 14-19 year-old females and was associated with an early age of first sexual contact and increasing number of lifetime sexual partners [25]. The prevalence in our study might have been underestimated due to the long storage time between taking of specimens being taken and testing.…”

Section: Discussionmentioning

confidence: 96%

“…Furthermore, 'age-bridgers' engaged in more risky sexual behaviour in a cohort of CT-positive heterosexual young men aged 14-24 years [33]. Other reasons for higher prevalence in young girls could be cervical ectopy [34], earlier sexual debut or more partners during this earlier period of sexual experience [23,25]. Poor healthcare-seeking behaviour associated with higher infection rates, lower partner referral or inadequate care have been reported for people with lower socio-economic status in many countries [35][36][37]: our findings of a higher prevalence in girls with low or medium social status are therefore not surprising.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Risk factors for Chlamydia trachomatis infection in adolescents: results from a representative population-based survey in Germany, 2003–2006

et al. 2013

View full text Add to dashboard Cite

Infections with Chlamydia trachomatis (CT) can lead to severe sequelae; however, they are not notifiable in Germany. We tested urine samples from participants of KiGGS (German Health Interview and Examination Survey for Children and Adolescents) for CT infections and linked the results to demographic and behavioural data from 1,925 participants (girls aged 15-17 years and boys aged 16-17 years) to determine a representative prevalence of CT infection in adolescents in Germany and to assess associated risk factors. Prevalence of CT infection was 2.2% (95% CI: 1.4-3.5) in girls and 0.2% (95% CI: 0.1-0.7) in boys. CT infection in girls was associated with higher use of alcohol, marijuana and cigarettes, lower social status, oral contraceptive use, pregnancy, repeated lower abdominal pain and higher rates of doctors' consultations within the preceding three months and consultation of gynaecologists within the last 12 months. In multiple logistic regression, we identified two predictors for CT infection: marijuana consumption often or several times within the last 12 months (F(1,164)=7.56; p<0.05) and general health status less than 'very good' (F(1,164)=3.83; p=0.052). Given our findings, we recommend enhancing sex education before sexual debut and promoting safe sex practices regardless of the contraceptive method used. Well-informed consumption of alcohol should be promoted, the risky behaviour of people intoxicated through consumption of marijuana highlighted and doctors' awareness of CT screening enhanced.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Risk factors for Chlamydia trachomatis infection in adolescents: results from a representative population-based survey in Germany, 2003–2006

et al. 2013

View full text Add to dashboard Cite

show abstract

“…[39][40][41] However, it was subsequently shown that sequence variation of cryptic plasmids has been detected in several countries. [42][43][44] This demonstrates that false-negative results may occur when a cryptic plasmid is used alone as the target for detection of C. trachomatis particularly when screening clinical samples from this region. Previously, several commercial NAATs used a single target on the pathogens associated with sexually transmitted infections, which led to the emergence of a false-negative diagnostic result, and the infection went untreated and was transmitted between individuals without knowledge before being recognized.…”

Section: Discussionmentioning

confidence: 97%

<p>Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis</p>

Xiu¹,

Zhang²,

Li³

et al. 2019

IDR

View full text Add to dashboard Cite

PurposeSexually transmitted infections (STIs), representing a major global health problem, are caused by different microbes, including bacteria, viruses, and protozoa. Unfortunately, infections of different sexually transmitted pathogens often present similar clinical symptoms, so it is almost impossible to distinguish them clinically. Therefore, the aim of the current study was to develop a sensitive, multitarget, and high-throughput method that can detect various agents responsible for STIs.MethodsWe developed and tested a 23-plex PCR coupled with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) assay (sexually transmitted infection-mass spectrometry, STI-MS) that simultaneously targets 11 different agents, including 8 most common clinical pathogens related to STIs (HSV-1, HSV-2, Neisseria gonorrhoeae, Chlamydia trachomatis, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, and Haemophilus ducreyi) and 3 controversial microorganisms as pathogens (Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum).ResultsThe results showed that the STI-MS approach can accurately detect the expected agents, without cross-reaction with other organisms. The limit of detection of each STI-MS assay was ranged from 1.739 to 10.009 copies/reaction, using probit analyses. The verification rate for each target organism of the STI-MS ranged from a minimum of 89.3% to a maximum of 100%, using conventional assays and ultrasensitive digital PCR to confirm the STI-MS-positive results. To further evaluate the clinical performance of this assay, 241 clinical specimens (124 urethral/cervical swabs and 117 urine) were tested in parallel using the STI-MS assay and monoplex real-time PCR for each agent. The overall validation parameters of STI-MS were extremely high including sensitivity (from 85.7% to 100%), specificity (from 92.3% to 100%), PPV (from 50% to 100%), and NPV (from 99.1% to 100%) for each target.ConclusionSTI-MS is a useful high-throughput screening tool for detecting mixed infections of STIs and has great potential for application in large-scale epidemiological programs for specific microorganisms of STI.

show abstract

“…Human (n = 172) and murine (n = 4) serum samples were obtained from serum collections of previous studies (Essig et al, 1999;Fieser et al, 2013;Forsbach-Birk et al, 2013;Forsbach-Birk et al, 2010;Wellinghausen et al, 2006) and from patients with respiratory tract infections (bronchitis, pneumonia) submitted for diagnostic work-up to the MVZ Labor Ravensburg. All samples were anonymized and their use was approved by the Ethics Committee of the University of Ulm (96/09).…”

Section: Serum Samplesmentioning

confidence: 99%

Analysis of humoral immune responses to recombinant Chlamydia pneumoniae antigens

Hagemann

Simnacher

Marschall

et al. 2020

International Journal of Infectious Diseases

View full text Add to dashboard Cite

Objectives: Chlamydia pneumoniae is a difficult to diagnose respiratory pathogen. This study was performed to systematically characterize humoral immune responses to selected C. pneumoniae antigens in order to provide novel serodiagnostic perspectives for clinical and epidemiological issues. Methods: Based on a literature search, gene library screening, and serological proteome analysis, 15 immunogenic surface-associated, virulence-associated, and hypothetical C. pneumoniae antigens were selected, recombinantly expressed, and lined on a nitrocellulose strip. Specific IgM and IgG reactivity was measured in a total of 172 PCR-and micro-immunofluorescence testing (MIF)-characterized serum samples from patients with respiratory infections. A theoretical model was conceived to approximate a putative course of C. pneumoniae antigen expression and assess the potential of early and late antigens. Results: While surface antigens performed poorly, the virulence-associated TARP was a reliable antigen for IgM detection, with a sensitivity of 80.0% and a diagnostic specificity of 90.2%. The hypothetical protein YwbM proved powerful for IgG detection with MIF-correlative sensitivities of up to 94.4% and a diagnostic specificity of 95.1%. Conclusions: This study provides new insights into antibody profiles to immunogenic proteins in C. pneumoniae infection. The study findings offer antigen candidates for more reliable and standardized serological investigations of C. pneumoniae infections, including studies on seroprevalence and epidemiology.

show abstract

Chlamydia trachomatis prevalence, genotype distribution and identification of the new Swedish variant in Southern Germany

Cited by 16 publications

References 28 publications

Risk factors for Chlamydia trachomatis infection in adolescents: results from a representative population-based survey in Germany, 2003–2006

Risk factors for Chlamydia trachomatis infection in adolescents: results from a representative population-based survey in Germany, 2003–2006

<p>Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis</p>

Analysis of humoral immune responses to recombinant Chlamydia pneumoniae antigens

Contact Info

Product

Resources

About